Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences

Chansiri, Kosum; Kawazu, Shin-ichiro; Kamio, Tsugihiko; Terada, Yutaka; Fujisaki, Kozo; Piégay, Hervé; Sarataphan, Nopporn

doi:10.1016/s0304-4017(99)00052-7

Cited by 34 publications

(26 citation statements)

References 16 publications

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“…T. buffeli D-like were found in 3 buffalo samples and is similar to T. buffeli type D and T. sp. (Thung Song) found in cattle in Thailand, the USA and Korea (Chae et al 1999;Chansiri et al 1999) and a sequence designated as T. sp. MK from sheep and goats in Turkey (Altay et al 2007), but differed by at least 8 nucleotide substitutions.…”

Section: The T Buffeli Cladementioning

confidence: 99%

Diversity in the 18S SSU rRNA V4 hyper-variable region ofTheileriaspp. in Cape buffalo (Syncerus caffer) and cattle from southern Africa

et al. 2011

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Sequence variation within the 18S SSU rRNA V4 hyper-variable region can affect the accuracy of real-time hybridization probe-based diagnostics for the detection of Theileria spp. infections. This is relevant for assays that use non-specific primers, such as the real-time hybridization assay for T. parva (Sibeko et al. 2008). To assess the effect of sequence variation on this test, the Theileria 18S gene from 62 buffalo and 49 cattle samples was cloned and *1000 clones sequenced. Twentysix genotypes were detected which included known and novel genotypes for the T. buffeli, T. mutans, T. taurotragi and T. velifera clades. A novel genotype related to T. sp. (sable) was also detected in 1 bovine sample. Theileria genotypic diversity was higher in buffalo compared to cattle. Polymorphism within the T. parva hyper-variable region was confirmed by aberrant real-time melting peaks and supported by sequencing of the S5 ribosomal gene. Analysis of the S5 gene suggests that this gene can be a marker for species differentiation. T. parva, T. sp. (buffalo) and T. sp. (bougasvlei) remain the only genotypes amplified by the primer set of the hybridization assay. Therefore, the 18S sequence diversity observed does not seem to affect the current real-time hybridization assay for T. parva.

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Section: The T Buffeli Cladementioning

confidence: 99%

Diversity in the 18S SSU rRNA V4 hyper-variable region ofTheileriaspp. in Cape buffalo (Syncerus caffer) and cattle from southern Africa

et al. 2011

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“…A significant proportion of the systematic study of the Piroplasmorida comprise analysis of the 18S rRNA gene (Allsopp et al 1994;Chae et al 1999;Chansiri et al 1999;Criado-Fornelio et al 2004;Reichard et al 2005;Criado et al 2006;Altay et al 2007;Bhoora et al 2009). The reverse line blot (RLB) method (Gubbels et al 1999), based on simultaneous detection of the 18S rRNA gene for various Babesia and Theileria species, has been extensively used for surveillance purposes (Georges et al 2001;Almerìa et al 2002;Oura et al 2004;Nijhof et al 2003Nijhof et al , 2005Altay et al 2007;M'ghirbi et al 2008;Altay et al 2008;Matjila et al 2008;Oosthuizen et al 2008;Almerìa et al 2009;Salih et al 2010;Yusufmia et al 2010;Tomassone et al 2012;Ceci et al 2014;Githaka et al 2014;Eygelaar et al 2015;Njiiri et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach

Mans

Pienaar

Ratabane

et al. 2016

Ticks and Tick-borne Diseases

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Molecular classification and systematics of the Theileria is based on the analysis of the 18S rRNA gene. Reverse line blot or conventional sequencing approaches have disadvantages in the study of 18S rRNA diversity and a next-generation 454 sequencing approach was investigated. The 18S rRNA gene was amplified using RLB primers coupled to 96 unique sequence identifiers (MIDs). Theileria positive samples from African buffalo (672) and cattle (480) from southern Africa were combined in batches of 96 and sequenced using the GS Junior 454 sequencer to produce 825711 informative sequences. Sequences were extracted based on MIDs and analysed to identify Theileria genotypes. Genotypes observed in buffalo and cattle were confirmed in the current study, while no new genotypes were discovered.Genotypes showed specific geographic distributions, most probably linked with vector distributions. Host specificity of buffalo and cattle specific genotypes were confirmed and prevalence data as well as relative parasitemia trends indicate preference for different hosts.Mixed infections are common with African buffalo carrying more genotypes compared to cattle. Associative or incongruent co-infection profiles were observed between genotypes that may have implications for speciation and systematics: specifically that more Theileria species may exist in cattle and buffalo than currently recognized. Analysis of primers used for Theileria parva diagnostics indicate that no new genotypes will be amplified by the current primer sets confirming their specificity. Theileria parva SNP variants that occur in the 18S rRNA hypervariable region were confirmed. A next generation sequencing approach is useful in obtaining comprehensive knowledge regarding 18S rRNA diversity and prevalence for the Theileria, allowing for the assessment of systematics and diagnostic assays based on the 18S gene.Keywords: 18S SSU, Diversity, Next-generation sequencing, species, Theileria, Theileria parva 3 IntroductionThe Babesia and Theileria are part of the phylum Apicomplexa (Order Piroplasmorida) (Mans et al. 2015 A significant proportion of the systematic study of the Piroplasmorida comprise analysis of the 18S rRNA gene (Allsopp et al. 1994;Chae et al. 1999;Chansiri et al. 1999;Criado-Fornelio et al. 2004;Reichard et al. 2005;Criado et al. 2006;Altay et al. 2007;Bhoora et al. 2009). The reverse line blot (RLB) method (Gubbels et al. 1999), based on simultaneous detection of the 18S rRNA gene for various Babesia and Theileria species, has been extensively used for surveillance purposes (Georges et al. 2001; Almerìa et al. 2002;Oura et al. 2004;Nijhof et al. 2003Nijhof et al. , 2005Altay et al. 2007;M'ghirbi et al. 2008;Altay et al. 2008;Matjila et al. 2008;Oosthuizen et al. 2008; Almerìa et al. 2009;Salih et al. 2010;Yusufmia et al. 2010; Tomassone et al. 2012;Ceci et al. 2014;Githaka et al. 2014;Eygelaar et al. 2015;Njiiri et al. 2015). The method relies on the amplification of the V4 hypervariable region of the 18S rRNA gene, followed by hybridization t...

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“…They have a cosmopolitan distribution and infect cattle and buffalo in Africa, Australia, Asia, Europe and the United States of America (USA) (Chae et al, 1998;Chansiri et al, 1999;Cossio-Bayugar et al, 2002;Sarataphan et al, 2003;Aktas et al, 2007;Altay et al, 2008;M'ghirbi et al, 2008;Gimenez et al, 2009, Liu et al, 2010a, Wang et al, 2010. The cosmopolitan distribution of these species has been attributed to the global movement of cattle (and buffalo) without any regard to infection, and therefore their distribution mainly depends on the availability of a suitable tick vector (Chae et al, 1999c;Cossio-Bayugar et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…The classification of these benign parasites is still confusing and is complicated by their similar morphology, serology, vector transmission, geographical distribution, difficulties in obtaining pure isolates and incompletely understood life-cycles (Uilenberg et al, 1985;Chae et al, 1999c;Chansiri et al, 1999;Yin et al, 2004;M'ghirbi et al, 2008M'ghirbi et al, , Uilenberg, 2011. It is still unclear if these organisms represent the same species or different species.…”

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Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Chaisi

Collins

Oosthuizen

2014

Veterinary Parasitology

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Theileria buffeli/T. sergenti/T. orientalis is a group of benign and mildly pathogenic species of cattle and buffalo. In a previous study, we identified T .buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe-iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterize the 18S rRNA gene and complete internal transcribed spacer (ITS1-5.8S-ITS2) region of T. buffeli samples, and to establish the phylogenetic position of this species based on these loci. The 18S rRNA gene and the complete ITS region were amplified from DNA extracted from blood samples originating from buffalo in these localities. The PCR products were cloned and the resulting recombinants sequenced. We identified novel T. buffeli 18S rRNA gene and ITS genotypes from buffalo in the AEGP, and novel T. sinensis 18S rRNA genotypes from buffalo in the HIP. Phylogenetic analyses indicated that the T. buffeli-like sequences were similar to T. buffeli sequences from cattle and buffalo in China and India, and the T. sinensis-like sequences were similar to T. sinensis 18S rRNA sequences of cattle and yak in China. There was extensive sequence variation between the novel T.1 buffeli genotypes of the African buffalo and previously described T. buffeli and T. sinensis genotypes. The presence of organisms with T. buffeli-like and T. sinensis-like genotypes in the African buffalo could be of significant importance, particularly to the cattle industry in South Africa as these animals might act as sources of infections to naïve cattle. This is the first report on the characterization of the full-length 18S rRNA gene and ITS region of T. buffeli and T. sinensis genotypes in South Africa. Our study provides invaluable information towards the classification of this complex group of benign and mildly pathogenic species.

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Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences

Cited by 34 publications

References 16 publications

Diversity in the 18S SSU rRNA V4 hyper-variable region ofTheileriaspp. in Cape buffalo (Syncerus caffer) and cattle from southern Africa

Diversity in the 18S SSU rRNA V4 hyper-variable region ofTheileriaspp. in Cape buffalo (Syncerus caffer) and cattle from southern Africa

Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

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