Molecular phylogeny and genetic diversity of Tunisian Quercus species using chloroplast DNA CAPS markers

Caps DNA Barcoding for Field Laboratory Identification of Grass Species (British Grasses as a Model)

“…Initial success came from the use of a highly variable 648-bp region near the 5'end of the cytochrome oxidase subunit I (COI) gene led to the application of the approach on a wide diversity of animal species including birds (Hebert et al 2004), amphibians (Vences et al 2005), fishes (Ward et al 2005) insects (Nelson et al 2007), and mammals (Lorenz et al 2005). However, the COI gene performed poorly for plant species diagnosis, partly because genes of the mitochondrial genome of plants evolve extremely slowly ( Chloroplast-derived Cleaved Amplified Polymorphic Sequence (CAPS) markers have also been reported to detect the interspecies variation of different plant species (e.g., Sakka et al 2015), and hence it has been applied to identify several groups of plant species including grasses. For instance, Kim et al (2018) reported that differences revealed between Pinus sylvestris and P. densiflora based on cpDNA-CAPS can be used to distinguish between these two species.…”