A fungal strain designated KNUF-22-014, belonging to the family
Ophiocordycipitaceae
, was isolated from a soil sample collected in Sobaeksan National Park, South Korea. Phylogenetic trees based on the combined sequences of internal transcribed spacer (ITS) regions and the large subunit of 28S rRNA (
LSU
) gene, as well as on five concatenated loci (
LSU
, ITS, translation elongation factor 1α, β-tubulin, and subunit 1 of RNA polymerase II), revealed that the isolate falls within the clade containing the genus
Tolypocladium
but occupies a distinct phylogenetic position. Its closest relative is
T. tropicale
. Morphologically, strain KNUF-22-014 can be distinguished from
T. tropicale
by the shape of its phialides (subcylindrical vs. lageniform), its conidiophore type (arborescent or unbranched vs. trichodermatoid), and its larger conidia size (2.1 ± 0.1 µm vs. 1.5 ± 0.1 µm). Phylogenetic analysis and morphological observations indicate that KNUF-22-014 represents a novel species in the genus
Tolypocladium
, for which the name
Tolypocladium sobaeksanense
sp. nov. is proposed.