Molecular phylogeny based on six nuclear genes suggests that Echinococcus granulosus sensu lato genotypes G6/G7 and G8/G10 can be regarded as two distinct species

Laurimäe, Teivi; Kinkar, Liina; Moks, Epp; Römig, Thomas; Omer, Rihab Ali; Casulli, Adriano; Umhang, Gérald; Bagrade, Guna; Irshadullah, Malik; Sharbatkhori, Mitra; Mirhendi, Hossein; Ponce-Gordo, Francisco; Soriano, Silvia Viviana; Varcasia, Antonio; Rostami‐Nejad, Mohammad; Andresiuk, Vanessa; Saarma, Urmas

doi:10.1017/s0031182018000719

Cited by 74 publications

(43 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cases of CE in humans have been reported worldwide for more than 50 years. Until recently, the genus Echinococcus consisted of 9 valid species, including the livestock-friendly species [E. granulosus s.s. (G1 and G3), E. equinus (G4), E. ortleppi (G5), and E. canadensis (G6 and G7); species name is controversial ] and the wild animal-friendly species [E. canadensis (G8, G10), E. felidis, E. multilocularis, E. shiquicus, E. vogeli, and E. oligarthra] [7,8,[17][18][19]. It is worth noting that genetic classification of isolates from both definitive and intermediate hosts can contribute to programs aimed to control this public health problem by determining how these organisms maintain their life cycle.…”

mentioning

confidence: 99%

Phylogenetic Characteristics of Echinococcus granulosus Sensu Lato in Uzbekistan

et al. 2020

View full text Add to dashboard Cite

Echinococcosis occurs mainly in areas with heavy livestock farming, such as Central Asia, America, and Australia. Echinococcus granulosus sensu lato (s.l.) infection causes echinococcosis in intermediate hosts, such as sheep, cattle, goats, camels, and horses. Numerous cases of echinococcosis occur in Uzbekistan as stock farming is a primary industry. Epidemiological and genetic studies of E. granulosus s.l. are very important for mitigating its impact on public health and the economy; however, there are no such studies on E. granulosus s.l. in Uzbekistan. In the present study, to determine which genotypes exist and are transmitted, we isolated Echinococcus sp. from definitive hosts (one isolate each from jackal and dog) and intermediate hosts (52 isolates from humans and 6 isolates from sheep) in Uzbekistan and analyzed the isolates by sequencing 2 mitochondrial DNA components (cox1 and nad1). The results showed that all of isolates except one belonged to the E. granulosus sensu stricto (s.s.) G1 and G3 genotypes. Phylogenetic analysis based on cox1 sequences showed that 42 isolates from humans, 6 isolates from sheep, and one isolate from jackal were the G1 genotype, whereas the remaining 8 isolates from human and the one isolate from dog were the G3 genotype. These results suggest that the G1 and G3 genotypes of E. granulosus s.s. are predominant in Uzbekistan, and both wild animals and domestic animals are important for maintaining their life cycle. Only one isolate from human sample was confirmed to be E. eqiinus (G4 genotype), which is known to be for the first time.

show abstract

mentioning

confidence: 99%

Phylogenetic Characteristics of Echinococcus granulosus Sensu Lato in Uzbekistan

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Interestingly, DNA of G-Omo did not react in the E. granulosus s.s. qPCR (as E. felidis , which belongs to the same species cluster), which supports a separate identity of this taxon. Likewise, E. canadensis may in future have to be split in two species, namely the wildlife-transmitted G8 and G10, and the largely domestically transmitted G6/7 genotype group [ 29 ]. This will not diminish the value of the qPCR, as both genotype clusters are allopatrically distributed and occur in very different epidemiological settings, with only a limited area of possible overlap in the Asian part of the Russian Federation [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs

et al. 2020

Self Cite

View full text Add to dashboard Cite

Infections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the E. granulosus s.l. complex. It is therefore essential to diagnose the correct species within E. granulosus s.l. to help understand specific disease epidemiology and to facilitate effective implementation of control measures. For this purpose, simple, fast and cost-effective typing techniques are needed. We developed quantitative real-time polymerase chain reactions (qPCRs) to target polymorphic regions in the mitochondrial genome of E. granulosus s.l. In a single-step typing approach, we distinguished E. granulosus s.l. members in four epidemiologically relevant subgroups. These were E. granulosus sensu stricto, E. equinus, E. ortleppi and the E. canadensis cluster. The technique also allowed identification and differentiation of these species from other Echinococcus or Taenia taxa for samples isolated from cysts or faeces.

show abstract

“…Interestingly, DNA of G-Omo did not react in the E. granulosus s.s. qPCR (as E. felidis, which belongs to the same species cluster), which supports a separate identity of this taxon. Likewise, E. canadensis may in future have to be split in two species, namely the wildlife transmitted G8 and G10, and the largely domestically transmitted G6/7 genotype group [37]. This will not diminish the value of the qPCR, as both genotype cluster are allopatrically distributed and occur in very different epidemiological settings, with only a limited area of possible overlap in the Asian part of the Russian Federation [4].…”

Section: Discussionmentioning

confidence: 99%

Species detection within the Echinococcus granulosus sensu lato complex by novel probe-based Real-Time PCRs

Maksimov¹,

Bergmann²,

Wassermann³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Infections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the E. granulosus s.l. complex. It is therefore essential to diagnose the correct species within E. granulosus s.l. to help understand specific disease epidemiology and to facilitate effective implementation of control measures. For this purpose, simple, fast and cost-effective typing techniques are needed.Methods: Mitochondrial genome sequences from the respective members of E. granulosus s.l. complex were used to identify target regions for the detection and genotyping. The selected primer pairs were first tested in a SYBR-green assay. In the next step, primers discriminating between the genotypes (according to post-amplification melting curves) were tested together with the respective probes in a TaqMan® Real-Time Polymerase Chain Reactions (qPCRs). For the analysis of analytical sensitivity and specificity of the real-time PCRs (SYBR-green and TaqMan), a panel of reference DNAs from various Echinococcus spp. was used.Results: We developed quantitative qPCRs and corresponding sequence-specific hydrolysis DNA probes to target polymorphic regions in the mitochondrial genome of E. granulosus s.l.. In a single-step typing approach, we distinguished E. granulosus s.l. members in four epidemiologically relevant subgroups. These were E. granulosus sensu stricto (G1, G3), E. equinus (G4), E. ortleppi (G5) and the E. canadensis cluster (G6 to G8 and G10). The technique also allowed identification and differentiation of these species from other Echinococcus or Taenia taxa for samples isolated from cysts or faeces. Conclusions: Single-step genotyping techniques for the molecular diagnosis of Echinococcus spp. by qPCRs may not only improve diagnostic performance, but also our knowledge on the epidemiology of the parasites and help controlling the various agents of cystic echinococcosis.

show abstract

Molecular phylogeny based on six nuclear genes suggests that Echinococcus granulosus sensu lato genotypes G6/G7 and G8/G10 can be regarded as two distinct species

Cited by 74 publications

References 62 publications

Phylogenetic Characteristics of Echinococcus granulosus Sensu Lato in Uzbekistan

Phylogenetic Characteristics of Echinococcus granulosus Sensu Lato in Uzbekistan

Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs

Species detection within the Echinococcus granulosus sensu lato complex by novel probe-based Real-Time PCRs

Contact Info

Product

Resources

About