Molecular phylogeny of Anopheles hyrcanus group members based on ITS2 rDNA

Fang, Yuan; Shi, Wei; Zhang, Yi

doi:10.1186/s13071-017-2351-x

Cited by 38 publications

(45 citation statements)

References 49 publications

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“…hyrcanus from An . pseudopictus , which is in accordance with a previous report [ 33 ]. Regarding the current genetic analyses, we agree with previous suggestions not to consider An .…”

Section: Discussionsupporting

confidence: 94%

DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran

et al. 2018

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Identifying mosquito species is a fundamental step in risk assessment and implementation of preventative strategies. Moreover, Culex pipiens is the most widespread mosquito vector in several regions of Iran and is the main vector for transmission of West Nile virus (WNV). Mosquitoes were collected at 14 sites in northern regions of Iran in 2015 and 2016. A subset of mosquito specimens was selected for identification confirmation using a DNA-barcoding technique. Construction of a phylogenetic tree showed clustering of mosquito sequences into three main genera: Aedes, Anopheles and Culex with individuals of a single species clustered closely together, regardless of where and when they were collected. Cx. pipiens complex and Cx. torrentium were identified and differentiated using multiplex real-time PCR targeting the gene locus for acetylcholinesterase 2 (ace2) to discriminate between Cx. pipiens pipiens and Cx. torrentium. The CQ11 microsatellite locus was used for discrimination between Cpp. biotypes. The predominant mosquito species in investigated regions were Cx. pipiens pipiens biotype pipiens, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes, as well as Cx. torrentium. The results of this study represent the first certain evidence of the presence of Cx. pipiens pipiens biotype molestus and hybrids between pipiens and molestus forms, and Cx. torrentium in Iran through a molecular identification approach. This report of a potentially important bridge vector for WNV might have key influence in the risk projections for WNV in Iran.

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“…hyrcanus from An . pseudopictus , which is in accordance with a previous report [ 33 ]. Regarding the current genetic analyses, we agree with previous suggestions not to consider An .…”

Section: Discussionsupporting

confidence: 94%

DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran

et al. 2018

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“…After collection, mosquitoes were identified using morphological characteristics according to the national key [ 38 ]. Some morphological confused specimens were determined by molecular methods, as reported previously [ 39 , 40 ]. Mosquitoes were then pooled by species, sex, and date and location of collection, with one to 50 individuals per pool.…”

Section: Methodsmentioning

confidence: 99%

Co-circulation of Aedes flavivirus, Culex flavivirus, and Quang Binh virus in Shanghai, China

et al. 2018

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BackgroundWith increases in global travel and trade, the spread of arboviruses is undoubtedly alarming. Pathogen detection in field-caught mosquitoes can provide the earliest possible warning of transmission. Insect-specific flavivirus (ISFV) has been first detected in 1991 and documented worldwide in the latest ten years. Although infection with ISFVs is apparently limited to insects, an increase in the infection rate of mosquito-borne flaviviruses may be able to induce cytopathic effects in vertebrate cells during co-infection with other human pathogens. However, little is known whether ISFVs persist in most regions of China.MethodsDuring the mosquito activity season in 2016, a surveillance program was carried out to detect ISFVs in mosquitoes in metropolitan Shanghai, China. The presence of ISFVs was randomly tested in different species of mosquitoes using RT-PCR-based and hemi-nested PCR assays, following by the sequencing of PCR products. Sequences from positive pooled samples were compared with those deposited in GenBank. Thereafter, sequences of representative insect flaviviruses were used for further phylogenetic and molecular evolutionary analyses.ResultsOur investigations showed: (1) the presence of Aedes flavivirus (AEFV) in 11/161 pooled samples (nine pools in Songjiang District, one pool in Huangpu District, and one pool in Qingpu District) of Aedes albopictus, (2) the presence of Quang Binh virus (QBV) in 10/195 pooled samples (all in Chongming District) of Culex tritaeniorhynchus; and (3) the presence of Culex flavivirus (CxFV) in 9/228 pooled samples (six pools in Pudong New Area, two pools in Huangpu District, and one pool in Chongming District) of Cx. pipiens. Furthermore, phylogenetic analyses of the gene sequences of envelope proteins indicated that Shanghai CxFV strains belonged to the Asia/USA genotype. The overall maximum likelihood estimation values (and 95% confidence interval) for CxFV, QBV, and AEFV in mosquitoes collected in Shanghai in 2016 were 1.34 (0.66–2.45), 1.65 (0.87–2.85), and 1.51 (0.77–2.70) per 1000, respectively.ConclusionsThis study reveals the presence and the geographical distribution of ISFVs, and determines the genetic variation and the infection rate of ISFVs in Shanghai, China. At least, three insect flaviviruses including ISFVs, AEFV, CxFV, and QBV, co-circulate in this area. To our knowledge, this is the first report of AEFV in China.Electronic supplementary materialThe online version of this article (10.1186/s40249-018-0457-9) contains supplementary material, which is available to authorized users.

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“…They concluded that ITS2 would be more reliable than COI as a phylogenetic analysis tool among very close taxa. This discrepancy could be attributed to the fact that the Hyrcanus Group includes at least 25 species widely distributed in a large geographic area [15,16]. Discrepancies between markers are expected since there are different evolutionary processes that act differently on mitochondrial and nuclear genes [44].…”

Section: Discussionmentioning

confidence: 99%

“…Another information gap in Honduras is the lack of molecular data that support the classi cation of mosquitoes based on morphometric keys. Molecular markers are critical to distinguish between evolutionarily close or cryptic species, even using immature specimens [15,16].…”

Section: Introductionmentioning

confidence: 99%

Distribution and phylogenetic diversity of Anopheles species in malaria endemic areas of Honduras in an elimination setting

Escobar

Ascencio

Ortiz

et al. 2020

Preprint

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Abstract Background : Anopheles mosquitoes are the vectors of malaria, one of the most important infectious diseases in the tropics. More than 500 Anopheles species have been described worldwide, and more than 30 are considered a public health problem. In Honduras, information on the distribution of Anopheles spp. and its genetic diversity is scarce. This study aimed to describe the distribution and genetic diversity of Anopheles mosquitoes in Honduras. Methods : Mosquitoes were captured in 8 locations in 5 malaria endemic departments during 2019. Two collectionmethods were used. Adult anophelines were captured outdoors using CDC light traps and by aspiration of mosquitoes at rest. The morphological identification was performed using taxonomic keys. Genetic analyses included the sequencing of a partial region of the cytochrome oxidase I gene (COI) and the ribosomal internal transcribed spacer 2 (ITS2). Results : A total of 1320 anophelines were collected and identified through morphological keys. Seven Anopheles species were identified. Anopheles albimanus was the most widespread and abundant species (74.02%). To confirm the morphological identification of the specimens, 175 and 122 sequences were obtained for COI and ITS2 respectively. Both markers confirmed the morphological identification. COI showed a greater nucleotide diversity than ITS2 in all species. High genetic diversity was observed within the populations of An. albimanus while An. darlingi proved to be a highly homogeneous population. Phylogenetic analyses revealed clustering patterns in An. darlingi and An. neivai in relation to specimens from South America. New sequences for An. crucians , An. vestitipennis , and An. neivai are reported in this study. Conclusions : Here we report the distribution and genetic diversity of Anopheles species in endemic areas of malaria transmission in Honduras. According to our results, both taxonomic and molecular approaches are useful tools in the identification of anopheline mosquitoes. However, both molecular markers differ in their ability to detect intraspecific genetic diversity. These results provide supporting data for a better understanding of the distribution of malaria vectors in Honduras.

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Molecular phylogeny of Anopheles hyrcanus group members based on ITS2 rDNA

Cited by 38 publications

References 49 publications

DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran

DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran

Co-circulation of Aedes flavivirus, Culex flavivirus, and Quang Binh virus in Shanghai, China

Distribution and phylogenetic diversity of Anopheles species in malaria endemic areas of Honduras in an elimination setting

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