Molecular phylogeny of Galanthus (Amaryllidaceae) of Anatolia inferred from multiple nuclear and chloroplast DNA regions

Abstract

Anatolia hosts 14 of the 22 taxa of genus Galanthus L. The phylogenetic relationships of Galanthus species were explored using DNA sequence data from both nuclear and chloroplast DNA of 70 specimens covering all subdivisions of Galanthus present in Turkey. Our data showed that species of the same geography clustered together phylogenetically, in contrast to the presently recognized taxonomy based on morphological characters. The 5.8S rRNA sequence data by itself is sufficiently informative for constructing phylogenetic trees. Our data confirm the identity of Galanthus koenenianus as a new taxon, well separated from G. alpinus; both are clustered within subseries Viridifolii, rather than within subseries Glaucaefolii in contrast to previous suggestions, but in accordance with their geographical occurrence. G. trojanus also forms a distinct branch within subseries Glaucaefolii. G. krasnovii diverged out of all other Galanthus taxa as a separate branch in all phylogenetic trees. Samples collected based on morphological characters as G. nivalis were clearly different from G. nivalis based on the sequence data in GenBank. Sequencing data also revealed that the G. xvalentinei from locus classicus (ISTF; SYZB 3008) significantly differed from all other Galanthus specimens distributed in Thrace known as G.xvalentinei.

Galanthus bursanus (Amaryllidaceae): a new species of snowdrop from the Marmara Sea region, NW Turkey

“…byzantinus and G. nivalis (Davis et al 2001). Molecular data (Rønsted et al 2013;Taşcı Margoz et al 2013) support the assumption of hybrid origin for G. ×valentinei nothosubsp. subplicatus (Davis et al 2001), although the exact parentage is not yet known; the most detailed molecular study so far (Rønsted et al 2013) infers introgression between G. plicatus s.l.…”

“…Continued decline observed (Map 1). PHENOLOGY Taşcı Margoz et al 2013); and allopatric G. reginae-olgae subsp. reginae-olgae (Davis 1999;Davis 2001).…”

“…The Marmara Sea region in north-western Turkey (Asian and European parts) is an area of high diversity for Galanthus, with five indigenous taxa: G. gracilis Čelak., G. plicatus M.Bieb. subsp (Zeybek & Sauer 1995;Davis 1999;Davis 2001;Davis et al 2001;Davis & Özhatay 2001;Taşcı Margoz et al 2013). According to the work of Rønsted et al (2013) these taxa belong to three different phylogenetic clades: Trojanus (G. trojanus), Nivalis (G. plicatus and G. ×valentinei nothosubsp.…”

Phylogenetic analysis of six different species of Saraca L. (Fabaceae, Caesalpinioideae) based on chloroplast matK gene

“…Phylogenetic analysis of Chamaecrista (Torres et al 2011), Cistaceae (Aparicio et al 2017), Leucojum sp., and Galanthus sp. (Tasci et al 2013) was done effectively with the help of the sequence data of the matK gene. matK sequencing has been widely used in different Fabaceae plants, including Caesalpinioideae (Monkheang 2011), to evaluate discriminatory status (Khan et al 2016) and identification (Gao et al 2011).…”

“…The phylogenetic relationships between Leucojum sp., Galanthus sp. (Tasci et al 2013), and Cistaceae (Aparicio et al 2017), for example, were effectively determined via the application of the matK gene. The matK gene sequence is one of the most important potential markers for the establishment of phylogenetic correlations (Huo et al 2019;Yang et al 2018;Saha et al 2013).…”