Molecular phylogeny of Hypoxylon and closely related genera

Abstract

Phylogenetic relationships were inferred among several xylariaceous genera with Nodulisporium or nodulisporium-like anamorphs based on the analyses of beta-tubulin and alpha-actin sequences. One hundred nine cultures and three specimens of 83 representatives of these four genera were included in the study. Biscogniauxia taxa formed a well supported clade that was basal to the other taxa, while taxa of Hypoxylon and Daldinia comprised a large monophyletic group that contained two subclades. The first subclade encompassed Hypoxylon sect. Annulata and is accepted here as the new genus Annulohypoxylon. The second subclade contained taxa of Hypoxylon sect. Hypoxylon and Daldinia. Hypoxylon is restricted to include only those taxa in sect. Hypoxylon. Thirty-three epithets are made in Annulohypoxylon. Hypoxylon cohaerens var. microsporum is raised to the species level and accepted as A. minutellum. Hypoxylon polyporoideum is recognized as distinct from H. crocopeplum. Hypoxylon placentiforme is accepted as Daldinia placentiformis.

The Production of 1,8-Cineole, a Potential Biofuel, from an Endophytic Strain of <i>Annulohypoxylon</i> sp. FPYF3050 When Grown on Agricultural Residues

“…Annulohypoxylon was created as a new genus from Hypoxylon with ostioles and ascospora morphology and molecular phylogenety in 2005 [5] [38]. Annulohypoxylon is divided into two subclades based on the presence or absence of ostiolar disks [5]. In this study, the strain, FPYF3050, isolated as an endophytic fungus without any spores or other fruiting structures to characterize it, was characterized using its operational taxonomic units (OTU) species by four phylogenetic loci.…”

“…However, the best hits of the EF1-α of the strain queried in NCBI were Annulohypoxylon nitens and Annulohypoxylon sp. Annulohypoxylon was created as a new genus from Hypoxylon with ostioles and ascospora morphology and molecular phylogenety in 2005 [5] [38]. Annulohypoxylon is divided into two subclades based on the presence or absence of ostiolar disks [5].…”

“…Endophytic fungi occupy a significant proportion of untold numbers of potential novel fungal genera [2]. When morphological data are missing, one can use internal transcribed spacer (ITS) sequence data to aid in identification fungi [3] [4] [5]. For difference strains, the reliability of ITS identification alone is questionable [4] [5].…”

“…When morphological data are missing, one can use internal transcribed spacer (ITS) sequence data to aid in identification fungi [3] [4] [5]. For difference strains, the reliability of ITS identification alone is questionable [4] [5]. In recent years, multiple gene loci and even whole genome have been developed which greatly helps in organismal identification [3] [4] [5].…”

“…For difference strains, the reliability of ITS identification alone is questionable [4] [5]. In recent years, multiple gene loci and even whole genome have been developed which greatly helps in organismal identification [3] [4] [5]. Endophytic fungi are suitable for the discovery of new chemical entities including enzymes, and useful volatile organic compounds [6] [7] [8] [9] [10].…”