Molecular phylogeny of leader proteinase gene of type A of Foot-and-mouth disease virus from India

Tosh, C.; Mittal, Meenakshi; Sanyal, Aniket; Hemadri, D.; Bandyopadhyay, Santanu

doi:10.1007/s00705-003-0224-4

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…The phylogenetic tree representing the region encoding the L protein differed from that of the structural proteins where sub-grouping according to serotype was much less apparent, which was consistent with previous findings for this region [81, 86]. …”

Section: Discussionsupporting

confidence: 89%

Genetic heterogeneity in the leader and P1-coding regions of foot-and-mouth disease virus serotypes A and O in Africa

et al. 2013

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Genetic information regarding the leader (L) and complete capsid-coding (P1) region of FMD serotype A and O viruses prevalent on the African continent is lacking. Here, we present the complete L-P1 sequences for eight serotype A and nine serotype O viruses recovered from FMDV outbreaks in East and West Africa over the last 33 years. Phylogenetic analysis of the P1 and capsid-coding regions revealed that the African isolates grouped according to serotype, and certain clusters were indicative of transboundary as well as intra-regional spread of the virus. However, similar analysis of the L region revealed random groupings of isolates from serotypes O and A. Comparisons between the phylogenetic trees derived from the structural coding regions and the L region pointed to a possibility of genetic recombination. The intertypic nucleotide and amino acid variation of all the isolates in this study supported results from previous studies where the externally located 1D was the most variable whilst the internally located 1A was the most conserved, which likely reflects the selective pressures on these proteins. Amino acids identified previously as important for FMDV structure and functioning were found to be highly conserved. The information gained from this study will contribute to the construction of structurally designed FMDV vaccines in Africa.Electronic supplementary materialThe online version of this article (doi:10.1007/s00705-013-1838-9) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Genetic heterogeneity in the leader and P1-coding regions of foot-and-mouth disease virus serotypes A and O in Africa

et al. 2013

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“…Consistent with the complex relationships illustrated in Fig. 4A, analyses of nonstructural protein-encoding regions indicated groupings of serotypically disparate viruses, which extended previous but limited data (54,110,113) (Fig. 4C).…”

Section: N-terminal Regions Of 1b Contain Previously Undescribed Motisupporting

confidence: 87%

Comparative Genomics of Foot-and-Mouth Disease Virus

Carrillo

Tulman

Delhon

et al. 2005

J Virol

445

452

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Here we present complete genome sequences, including a comparative analysis, of 103 isolates of foot-andmouth disease virus (FMDV) representing all seven serotypes and including the first complete sequences of the SAT1 and SAT3 genomes. The data reveal novel highly conserved genomic regions, indicating functional constraints for variability as well as novel viral genomic motifs with likely biological relevance. Previously undescribed invariant motifs were identified in the 5 and 3 untranslated regions (UTR), as was tolerance for insertions/deletions in the 5 UTR. Fifty-eight percent of the amino acids encoded by FMDV isolates are invariant, suggesting that these residues are critical for virus biology. Novel, conserved sequence motifs with likely functional significance were identified within proteins L pro , 1B, 1D, and 3C. An analysis of the complete FMDV genomes indicated phylogenetic incongruities between different genomic regions which were suggestive of interserotypic recombination. Additionally, a novel SAT virus lineage containing nonstructural proteinencoding regions distinct from other SAT and Euroasiatic lineages was identified. Insights into viral RNA sequence conservation and variability and genetic diversity in nature will likely impact our understanding of FMDV infections, host range, and transmission.

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“…Only one Indian isolate (A ORS 64/84) could be grouped with the vaccine strain A IVRI which, in turn, could be grouped with A 10 HOLLAND 42. Similar fi ndings were reported by Tosh et al (2004) wherein the phylogenetic relationships between 35 Indian type A FMDV isolates were reconstructed by Lb gene sequences using quartet-puzzling steps. The possibility of the virus originating from outbreaks in animals that had been administered a vaccine containing partially or incompletely inactivated virus could not be ruled out.…”

Section: Phylogenetic Analysis Of Sequencessupporting

confidence: 80%

“…Unlike serotype O and Asia-1 virus, serotype A isolates have shown extensive antigenic variation (Arrowsmith 1975;Belwal et al 1986;Belwal et al 1987;Armstrong et al 1994;Azad et al 1995;Araujo et al 2002). Several workers (Tosh et al 2004;Mittal et al 2005;Jangra et al 2005;Mohapatra et al 2008) have studied genetic and antigenic variation in Indian FMDV isolates. Continuous monitoring of the disease is essential to understand the epidemiology of the virus and the mutations that occur over a period of time.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of foot-and-mouth disease virus serotype A of Indian origin and detection of positive selection and recombination in leader protease- and capsid-coding regions

et al. 2009

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The leader protease (L pro) and capsid-coding sequences (P1) constitute approximately 3 kb of the foot-and-mouth disease virus (FMDV). We studied the phylogenetic relationship of 46 FMDV serotype A isolates of Indian origin collected during the period 1968-2005 and also eight vaccine strains using the neighbour-joining tree and Bayesian tree methods. The viruses were categorized under three major groups -Asian, Euro-South American and European. The Indian isolates formed a distinct genetic group among the Asian isolates. The Indian isolates were further classi?ed into different genetic subgroups (<5% divergence).Post-1995 isolates were divided into two subgroups while a few isolates which originated in the year 2005 from Andhra Pradesh formed a separate group. These isolates were closely related to the isolates of the 1970s. The FMDV isolates seem to undergo reverse mutation or convergent evolution wherein sequences identical to the ancestors are present in the isolates in circulation. The eight vaccine strains included in the study were not related to each other and belonged to different genetic groups. Recombination was detected in the L pro region in one isolate (A IND 20/82) and in the VP1 coding 1D region in another isolate (A RAJ 21/96). Positive selection was identi?ed at aa positions 23 in the L pro (P < 0.05; 0.046*) and at aa 171 in the capsid protein VP1 (P < 0.01; 0.003**).

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Molecular phylogeny of leader proteinase gene of type A of Foot-and-mouth disease virus from India

Cited by 6 publications

References 42 publications

Genetic heterogeneity in the leader and P1-coding regions of foot-and-mouth disease virus serotypes A and O in Africa

Genetic heterogeneity in the leader and P1-coding regions of foot-and-mouth disease virus serotypes A and O in Africa

Comparative Genomics of Foot-and-Mouth Disease Virus

Genetic analysis of foot-and-mouth disease virus serotype A of Indian origin and detection of positive selection and recombination in leader protease- and capsid-coding regions

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