Molecular phylogeny of Siberian glossiphoniidae (Hirudinea)

Kaygorodova, Irina A.; Mandzyak, N. B.

doi:10.1134/s0026893314030091

Cited by 7 publications

(5 citation statements)

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“…These species inhabit exclusively freshwater bodies of all continents, except for Antarctica. Despite the rising interest in this group and the emergence of studies devoted to molecular evolution and systematics of leeches (e.g., de Carle, Oceguera‐Figueroa, Tessler, Siddall, & Kvist, 2017; Kaygorodova & Mandzyak, 2014; Moser, Richardson, & Hammond, 2012; Moser, Richardson, Hammond, & Lazo‐Wasem, 2011; Oceguera‐Figueroa, León‐Règagnon, & Siddall, 2011; Perez‐Flores, Rueda‐Calderon, Kvist, Siddall, & Oceguera‐Figueroa, 2016), the biodiversity of this annelid group is still considered understudied, especially in the Asian part of the Palaearctic. Currently, species composition of this leech group inhabiting Eastern Siberia, including the unique species of Baikal, consists of 15 species (Kaygorodova, 2012, 2015; Kaygorodova, Dzyuba, & Sorokovikova, 2013; Kaygorodova & Pronin, 2013; Kaygorodova & Utevsky, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The method hypothesizes the existence of a barcode gap visualizing a significant difference in the frequency distribution of intraspecific and interspecific variations of nucleotide composition in DNA marker sequences. As for molecular studies of leeches, the genetic barcodes have successfully discriminated both morphologically distinct and cryptic species within this group in the past (e.g., Bely & Weisblat, 2006; Kaygorodova & Mandzyak, 2014; Kutschera, 2011; Kvist, Oceguera‐Figueroa, Siddall, & Erseus, 2010; Nakano, 2012; Pfeiffer, Brenig, & Kutschera, 2004; Reyes‐Prieto et al, 2014; Siddall & Budinoff, 2005). In the present study, a standard phylogenetic marker—a fragment of mitochondrially encoded cytochrome c oxidase subunit I ( COI ) gene—is used for the genetic delimitation of Glossiphonia leech species from lakes and rivers of Eastern Siberia.…”

Section: Introductionmentioning

confidence: 99%

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Species delimitation through DNA barcoding of freshwater leeches of theGlossiphoniagenus (Hirudinea: Glossiphoniidae) from Eastern Siberia, Russia

Kaygorodova

Bolbat

2020

J. Zool. Syst. Evol. Res.

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The study of biodiversity is a priority task of biological science. The structural unit of biodiversity is a species that has a clear identification in a taxonomic system. Morphological features are traditionally the main criteria for species discrimination in zoological studies. However, the presence of inter‐ and intraspecific polymorphism and phenotypic plasticity makes it difficult to identify species in many groups of invertebrates. To solve this problem, in this research, we analyzed morphological and genetic data in combination to delimit species among the Eastern Siberia Glossiphonia leeches using different approaches. Morphology analysis revealed phenetically distinct groups, suggesting the existence of at least two species in the region, G. verrucata, a rare Palaearctic species, and a potentially new species Glossiphonia sp. Moreover, sequence‐based species delimitation methods congruently supported eight distinct species groups (including two Siberian species) within the available molecular dataset of the Glossiphonia world fauna, using phylogenetic (ML and BI), coalescent (ABGD and GMYC) methods, and pairwise analysis of sequences. The detected p‐distances (modal value of 0.11) between these 8 groups and the level of genetic polymorphism (max. 0.0041) within groups indicate that the groups are 8 independent species according to the DNA barcoding. Our results once again proved the usefulness of molecular systematics. At the same time, we detected several inaccuracies in the leech species identification, as well as many ambiguous sites in sequences uploaded on GenBank, which affects the analysis and impedes progress of DNA barcoding technology.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Species delimitation through DNA barcoding of freshwater leeches of theGlossiphoniagenus (Hirudinea: Glossiphoniidae) from Eastern Siberia, Russia

Kaygorodova

Bolbat

2020

J. Zool. Syst. Evol. Res.

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“…Nevertheless, DNA barcoding is actively being introduced, and although the technique is not universal, its usefulness remains undeniable. Previously, this tool was usefully applied to taxonomic issues in leeches [12][13][14][15][16][17][18][19][20][21][22]. Here, its utility in the taxonomically difficult but ecologically important freshwater genus Erpobdella De Blainville, 1818, was investigated.…”

Section: Introductionmentioning

confidence: 99%

Application of Modern Approaches to Species Delimitation in Freshwater Erpobdellidae (Hirudinea: Arhynchobdellida) with a New Interpretation of Their Genera

Kaygorodova

2024

Water

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The traditional taxonomy of freshwater invertebrates is a labor-intensive process requiring extensive knowledge and experience. In addition, this science is largely subjective, which makes its digitalization difficult. However, accurate species attribution is becoming increasingly important for environmental surveys and conservation efforts. In the 21st century, molecular biology methods have proven to be a successful tool for objectively determining biodiversity. Achieving objectivity in identifying the taxa of non-parasitic leeches of the family Erpobdellidae was the main goal of this study. To this end, various bioinformatic approaches to DNA sequence analysis have been tested. As a result, the phylogenetic diversity amounted to 47 species versus 29 morphospecies defined traditionally. The use of molecular species delimitation methods made it possible to identify seven complexes of morphologically hidden (cryptic) species and some morphological misidentifications, as well as to discover a new species from Eastern Siberia (Erpobdella sibirica) with a unique molecular diagnosis (DNA barcode). A pioneering attempt to apply the same approach to higher taxa showed that Erpobdellidae probably consists of seven genera, including the unambiguous elimination of the genus Motobdella. Obtaining quantitative and, therefore, objective data is an advantage of the molecular approach, which has proven to be effective in recognizing species and genera, at least in leeches.

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“…Traditionally, it was thought that one species in this genus, G. complanata (Linnaeus, 1758), has an enormous Holarctic range, expanding from Europe through Northeast Asia to North America [1-3]. However, a growing body of modern DNA-based research indicates that the distribution of this species is confined to Europe and that its records from Asia and North America should be attributed to other species [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Taxonomy and Melanism Patterns of Freshwater Leeches in the Genus Glossiphonia (Hirudinea: Glossiphoniidae) from Northeast Asia

et al. 2023

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Freshwater leeches belonging to the genus Glossiphonia Johnson, 1816 are fairly common benthic annelids in freshwater ecosystems throughout Eurasia, but the taxonomy of this group remains poorly resolved, and the species content of some local faunas is unsatisfactorily known. For example, it was thought that the only widespread species, Glossiphonia complanata (Linnaeus, 1758), dwells in Northeast Asia. Here, we revise the Northeast Asian Glossiphonia leeches with an integrative taxonomic approach. This revision is based on the largest DNA-sequence and morphological datasets collected to date. Two Glossiphonia species are recorded from the region. First, Glossiphonia koreaensissp. nov., a putative regional endemic species, was discovered in South Korea. Second, a valid name and complete description are provided for Glossiphonia mollissima Moore, 1898 (non-Grube, 1871). The latter name was wrongly applied through misidentification and, according to ICZN (Article 49), is unavailable. Hence, we propose a new name, Glossiphonia mooreisp. nov., in memory of Dr. J. Percy Moore, who discovered this leech. It is a prospective trans-Beringian species, ranging through Eastern Siberia, the Russian Far East, and Alaska (although Alaskan records are based on nonsequenced samples), showing a significant proportion of melanic individuals in samples north of the Arctic Circle. The latter pattern may reflect substrate-induced cryptic coloration (camouflage), but this hypothesis needs to be statistically checked in the future. Finally, a complete checklist of Glossiphonia species is presented.

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Molecular phylogeny of Siberian glossiphoniidae (Hirudinea)

Cited by 7 publications

References 6 publications

Species delimitation through DNA barcoding of freshwater leeches of theGlossiphoniagenus (Hirudinea: Glossiphoniidae) from Eastern Siberia, Russia

Species delimitation through DNA barcoding of freshwater leeches of theGlossiphoniagenus (Hirudinea: Glossiphoniidae) from Eastern Siberia, Russia

Application of Modern Approaches to Species Delimitation in Freshwater Erpobdellidae (Hirudinea: Arhynchobdellida) with a New Interpretation of Their Genera

Taxonomy and Melanism Patterns of Freshwater Leeches in the Genus Glossiphonia (Hirudinea: Glossiphoniidae) from Northeast Asia

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