Molecular phylogeny of species of Ligophorus (Monogenea: Dactylogyridae) and their affinities within the Dactylogyridae

Blasco‐Costa, Isabel; Míguez-Lozano, Raúl; Sarabeev, Volodimir; Balbuena, Juan Antonio

doi:10.1016/j.parint.2012.06.004

Cited by 53 publications

(67 citation statements)

References 56 publications

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“…Thus there is a large unpredictable component in the models imputable to a combination of measurement error, genetic variation, ontogenetic changes and plastic responses to environmental factors. The molecular analyses showed that the ITS1 sequences of our specimens were identical to those of L. cephali previously reported in a nearby locality (Cullera) by Blasco-Costa et al (2012). ITS1 sequences have previously shown some level of intraspecific divergence within species of monogeneans, including members of Gyrodactylus (0.09 -3.5% intraspecific divergence, Bueno-Silva et al, 2011), Lamellodiscus (0.27%, Desdevises et al, 2000) and Furnestinia (0.05 -1.38%, Mladineo et al, 2013).…”

Section: Ligophorus Llewellynisupporting

confidence: 82%

“…The DNA was extracted using an Qiagen DNeasy ® Blood & Tissue Kit following the manufacturer's instructions (Qiagen, Germany). ITS1 sequences were amplified using primers Lig18endF (5'-GTC TTG CGG TTC ACG CTG CT-3') and Lig5.8R (5'-GAT ACT CGA GCC GAG TGA TCC-3') (Blasco-Costa et al, 2012). PCR amplifications were performed in 20 µL reactions containing 2 µL of extracted DNA, the ready-to-use 2x MyFi Mix (Bioline Ltd., United Kingdom) and 5 pmol/µL of each primer.…”

Section: Molecular Datamentioning

confidence: 99%

“…The eight most complete new sequences generated in this work (GenBank accession numbers KP294376 -KP294383) and a previously published sequence of L. cephali from Blasco-Costa et al (2012) (GenBank accession number JN996865) were aligned for comparison using MUSCLE (Edgar, 2004) implemented in MEGA v5.1 (Tamura et al, 2011).…”

Section: Molecular Datamentioning

confidence: 99%

“…The aligned dataset of nine ITS1 sequences (eight sequences from this study and one from Blasco-Costa et al (2012) representing Ligophorus cephali) was composed of 630 nucleotide (nt) positions, after trimming the end parts to match the shortest sequence. This aligned sequence set showed exactly the same pattern of nts.…”

Section: Molecular Identificationmentioning

confidence: 99%

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Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach

Rodríguez-González

Míguez-Lozano

Llopis‐Belenguer

et al. 2015

International Journal for Parasitology

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Evaluating phenotypic plasticity in attachment organs of parasites can provide information on the capacity to colonize new hosts and illuminate evolutionary processes driving host specificity.We analysed the variability in shape and size of the dorsal and ventral anchors of Ligophorus cephali from Mugil cephalus by means of geometric morphometrics and multivariate statistics. We also assessed the morphological integration between anchors and between the roots and points in order to gain insight into their functional morphology. Dorsal and ventral anchors showed a similar gradient of overall shape variation, but the amount of localized changes was much higher in the former. Statistical models describing variations in shape and size revealed clear differences between anchors. The dorsal anchor/bar complex seems more mobile than the ventral one in Ligophorus, and these differences may reflect different functional roles in attachment to the gills. The lower residual variation associated with the ventral anchor models suggests a tighter control of their shape and size, perhaps because these anchors seem to be responsible for firmer attachment and their size and shape would allow more effective responses to characteristics of the microenvironment within the individual host. Despite these putative functional differences, the high level of morphological integration indicates a concerted action between anchors. In addition, we found a slight, although significant, morphological integration between roots and points in both anchors, which suggests that a large fraction of the observed phenotypic variation does not compromise the functional role of anchors as levers. Given the low level of genetic variation in our sample, it is likely that much of the morphological variation reflects host-driven plastic responses. This supports the hypothesis of monogenean specificity through host-switching and rapid speciation. The present study demonstrates the potential of geometric morphometrics to provide new and previously unexplored insights into the functional morphology of attachment and evolutionary processes of host-parasite coevolution.4

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Section: Ligophorus Llewellynisupporting

confidence: 82%

Section: Molecular Datamentioning

confidence: 99%

Section: Molecular Datamentioning

confidence: 99%

Section: Molecular Identificationmentioning

confidence: 99%

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Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach

Rodríguez-González

Míguez-Lozano

Llopis‐Belenguer

et al. 2015

International Journal for Parasitology

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“…The identification of species belonging to Ligophorus Euzet et Suriano, 1977, monogenean gill parasites of mullets (Mugilidae), mainly relies on the morphology and size of the sclerotised parts of the haptor and of the male copulatory complex (Blasco-Costa et al 2012). However, distinguishing between closely-related species may be very difficult, not only because of the small size of these structures, but also because of their close resemblance under optical microscopy.…”

mentioning

confidence: 99%

A comparative study of Ligophorus uruguayense and L. saladensis (Monogenea: Ancyrocephalidae) from Mugil liza (Teleostei: Mugilidae) in southern Brazil

Marchiori¹,

Pariselle²,

Pereira³

et al. 2015

Folia Parasitologica

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Representatives of Ligophorus Euzet et Suriano, 1977 were found on the gills of Mugil liza Valenciennes caught in southern Brazil. They were identified as Ligophorus uruguayense Failla Siquier et Ostrowski de Núñez, 2009 and Ligophorus saladensis Marcotegui et Martorelli, 2009, even though specific identification proved to be difficult due to inconsistencies in some diagnostic features reported for these two species. Therefore, a combined morphological and molecular approach was used to critically review the validity of these species, by means of phase contrast and confocal fluorescence microscopical examination of sclerotised hard parts, and assessing the genetic divergence between L. saladensis, L. uruguayense and their congeners using rDNA sequences. The main morphological differences between the two species relate to the shape of the accessory piece of the penis and the median process of the ventral bar. The accessory piece in L. uruguayense is shorter than in L. saladensis, has a cylindrical, convex upper lobe and straight lower lobe (vs with the distal tip of the lower lobe turning away from the upper lobe in the latter species). The ventral bar has a V-shaped anterior median part in L. uruguayense (vs U-shaped in L. saladensis). The two species are suggested to be part of a species complex together with L. mediterraneus Sarabeev, Balbuena et Euzet, 2005. We recommend to generalise such comparative assessment of species of Ligophorus for a reliable picture of the diversity and diversification mechanisms within the genus, and to make full use of its potential as an additional marker for mullet taxonomy and systematics.

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Evolutionary modularity and morphological integration in the haptoral anchor structures of Ligophorus spp. (Monogenea: Dactylogyridae)

et al. 2016

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An important question in the study of phenotypic evolution is whether characters are independent of each other or behave and evolve as integrated modules. Morphological integration and modularity provide a powerful framework for the analysis of the evolution of morphological traits. We used geometric morphometrics and phylogenetically independent contrasts (PIC) to test four different modularity hypotheses in the haptoral anchors of 14 monogenean species of Ligophorus. Integration between the modular units identified was further evaluated with two-block partial least squares analysis. Roots and points represented two modules in the dorsal and ventral anchors, but modularity was not statistically supported when parasite phylogeny was accounted for, which may indicate convergent evolution related to host characteristics and gill morphology. In contrast, PIC revealed medial and lateral modules in ventral anchors only. Moreover, we found evidence for ventral and dorsal anchor pairs forming two modules, supporting the notion that they play different functional roles. Integration between all identified modules was strong. We conclude that there is modular structure in the anchors of Ligophorus spp., accounted by adaptive and phylogenetic factors acting at different levels, and ventral and dorsal anchors evolve as integrated modules with specific roles in attachment.

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Molecular phylogeny of species of Ligophorus (Monogenea: Dactylogyridae) and their affinities within the Dactylogyridae

Cited by 53 publications

References 56 publications

Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach

Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach

A comparative study of Ligophorus uruguayense and L. saladensis (Monogenea: Ancyrocephalidae) from Mugil liza (Teleostei: Mugilidae) in southern Brazil

Evolutionary modularity and morphological integration in the haptoral anchor structures of Ligophorus spp. (Monogenea: Dactylogyridae)

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