Molecular phylogeny of theChironomus genus deduced from nucleotide sequences of two nuclear genes,ssp 160 and the globin 2b gene

Makarevich, I. F.; Berezikov, Eugène; Guryev, Victor; Блинов, А. Г.

doi:10.1007/bf02759569

Cited by 14 publications

(10 citation statements)

References 16 publications

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“…It seems unlikely that both groups show this similar pattern as a result of lineage sorting, whereas the presence of low levels of hybridization in both groups suggests gene flow as a more likely cause. This does not mean that mt gene flow will be common amongst Chironomus species, as our previous studies (Makarevich et al ., 2000; Guryev et al ., 2001) have revealed no obvious inconsistencies between mt and nuclear sequence data. There is also no evidence of mt gene flow between the two cryptic species included under the name C. calligraphus (Spies et al ., 2002).…”

Section: Discussionmentioning

confidence: 64%

“…4) are both consistent with the tree based on the gb2b sequences. There is further support for this tree from another nuclear gene, the ssp160 gene (Makarevich et al ., 2000). This gene is complete in C. pallidivittatus , in both the Nearctic and Palearctic, and in C. biwaprimus , while C. tentans (s.l.)…”

Section: Discussionmentioning

confidence: 81%

“…This gene is complete in C. pallidivittatus , in both the Nearctic and Palearctic, and in C. biwaprimus , while C. tentans (s.l.) from either continent contain a deletion of a large part of the gene (Makarevich et al ., 2000; S. T. Case, personal communication). It seems unlikely that such a deletion arose independently in each region, but rather that it occurred after the separation of C. tentans (s.l.)…”

Section: Discussionmentioning

confidence: 99%

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Population variability in Chironomus (Camptochironomus) species (Diptera, Nematocera) with a Holarctic distribution: evidence of mitochondrial gene flow

Martin¹,

Guryev²,

Блинов³

2002

Insect Molecular Biology

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Phylogenetic analysis of DNA sequences from mitochondrial (mt) genes (Cytochrome b and Cytochrome oxidase I) and one nuclear gene (globin 2b) was used for the investigation of Nearctic and Palearctic populations representing four Chironomus species of the subgenus Camptochironomus, namely C. biwaprimus, C. pallidivittatus, C. tentans sensu stricto and C. dilutus (the last two species constitute Holarctic C. tentans sensu lato). Phenograms constructed on the basis of mt sequences were not congruent with trees based on nuclear genes, or with morphological and cytological data. The mt tree divided the populations by continental region, rather than by the species groupings recognized by the other data sets. The incongruence is explained by mt gene flow resulting from hybridization between the sympatric species on each continent. Calculation of divergence times, based on the sequence data, suggest that C. tentans (s.l.) and C. pallidivittatus have both been in North America for about 2.5 My.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Population variability in Chironomus (Camptochironomus) species (Diptera, Nematocera) with a Holarctic distribution: evidence of mitochondrial gene flow

Martin¹,

Guryev²,

Блинов³

2002

Insect Molecular Biology

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“…Detailed genetic information, including cox1-RFLP sizes, the primers used to amplify the gb2β gene and whether or not the gb2β gene was amplified is given in Table 4. The gb2β gene of some Chironomus species includes an intron (type I or type II), whereas in others it is absent (Hankeln et al 1997;Makarevich et al 2000). This information is also given in Table 4.…”

Section: Species Delimitation and Identificationmentioning

confidence: 99%

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Proulx

Martin

Carew

et al. 2013

Zootaxa

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Chironomus Meigen (Diptera, Chironomidae) larvae are usually the largest sediment-burrowing chironomids, and as such often constitute a major part of the freshwater infaunal biomass. However, use of this genus in ecological, environmental and paleoecological studies is hampered by the fact that Chironomus larvae are difficult to identify to species because the larvae of many species are morphologically similar. We used a combination of morphological, cytological and genetic techniques to distinguish Chironomus larvae collected from 31 water bodies located in eastern Canada, producing 17 distinguishable groupings. These groups of larvae were ultimately identified as belonging to 14 known species (C. anthracinus, C. bifurcatus, C. cucini, C. decorus-group sp. 2, C. dilutus, C. entis, C. frommeri, C. harpi, C. maturus, C. nr. atroviridis (sp. 2i), C. ochreatus, C. plumosus, C. staegeri and C. 'tigris') and three other species that remain unidentified (C. sp. NAI-III). No single approach served to delimit and identify larvae of all 17 Chironomus species that we collected. Although we expected that morphological criteria alone would be insufficient, our results suggest that DNA barcoding, using either the mitochondrial cox1 or the nuclear gb2β gene, was also inadequate for separating some Chironomus species. Thus we suggest that multiple approaches will often be needed to correctly identify Chironomus larvae to species.

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“…The fragments of the vasa-related genes were amplified using degenerated primer pair VasFW and VasRV (Mochizuki et al, 2001). PCR was carried out in a volume of 30 ml using the standard technique with Taq polymerase at an annealing temperature of 48e55 C (Makarevich et al, 2000). Amplification products were separated in 1% agarose gel and then isolated using QIAquick Gel Extraction Kit (Qiagen) with subsequent subcloning into the pBluescript II SK vector and sequencing.…”

Section: Pcr Amplification Cloning and Sequencingmentioning

confidence: 99%

vasa‐related genes and their expression in stem cells of colonial parasitic rhizocephalan barnacle Polyascus polygenea (Arthropoda: Crustacea: Cirripedia: Rhizocephala)

Shukalyuk

Golovnina

Байбородин

et al. 2007

Cell Biology International

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vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-colonial Clistosaccus paguri (Crustacea: Cirripedia: Rhizocephala), and the parasitic isopodan Athelgis takanoshimensis (Crustacea: Isopoda). The colonial Polyascus polygenea, a parasite of the coastal crabs Hemigrapsus sanguineus and Hemigrapsus longitarsis was used as a model object for further detailed investigations. Phylogenetic analysis suggested that PpVLG and CpVLG are closely related to vasa-like genes of other Arthropoda. The rest of the studied genes form their own separate branch on the phylogenetic tree and have a common ancestry with the p68 and PL10 subfamilies. We suppose this group may be a new subfamily of the DEAD-box RNA helicases that is specific for parasitic Crustacea. We found PpVLG and PpDRH1 expression products in stem cells from stolons and buds of internae, during asexual reproduction of colonial P. polygenea, and in germ cells from sexually reproducing externae, including male spermatogenic cells and female oogenic cells.

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Molecular phylogeny of theChironomus genus deduced from nucleotide sequences of two nuclear genes,ssp 160 and the globin 2b gene

Cited by 14 publications

References 16 publications

Population variability in Chironomus (Camptochironomus) species (Diptera, Nematocera) with a Holarctic distribution: evidence of mitochondrial gene flow

Population variability in Chironomus (Camptochironomus) species (Diptera, Nematocera) with a Holarctic distribution: evidence of mitochondrial gene flow

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

vasa‐related genes and their expression in stem cells of colonial parasitic rhizocephalan barnacle Polyascus polygenea (Arthropoda: Crustacea: Cirripedia: Rhizocephala)

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