“…Two mitochondrial genes, 12S rRNA (12S) and 16S rRNA (16S), were PCR amplified from these samples using the primers and protocol published in Mausfeld and Schmitz (2003). These markers have been used extensively in molecular phylogenetic studies in lizards (Mausfeld et al 2003;Datta-Roy et al 2012) and are known to be variable and informative in resolving the relationships within squamates. A QIAquick PCR purification kit (Qiagen, Germany) was used to purify the PCR products and the sequences were obtained commercially from Eurofins Biotech (Bangalore, India).…”