Molecular physiological aspects of chalking mechanism in rice grains under high-temperature stress

Mitsui, Toshiaki; Yamakawa, Hiromoto; Kobata, Tohru

doi:10.1080/1343943x.2015.1128112

Cited by 45 publications

(34 citation statements)

References 65 publications

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“…Transgenic plants that suppress the expression of a-amylase genes by RNAi (Hakata et al, 2012) or the overexpression of manganese superoxide dismutase (MSD1) (Shiraya et al, 2015) reduced the number of chalky grains. This assumes that the balance between starch biosynthesis and degradation is regulated by reactive oxygen species and that imbalances in these processes, hypothesized to occur under high-temperature conditions, might lead to chalkiness (Mitsui et al, 2016).…”

Section: Mechanisms Underlying the Induction Of Rice Seed Chalkiness mentioning

confidence: 99%

FLOURY ENDOSPERM11‐2 encodes plastid HSP70‐2 involved with the temperature‐dependent chalkiness of rice (Oryza sativa L.) grains

et al. 2020

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Summary The frequent occurrence of chalky rice (Oryza sativa L.) grains becomes a serious problem as a result of climate change. The molecular mechanism underlying chalkiness is largely unknown, however. In this study, the temperature‐sensitive floury endosperm11‐2 (flo11‐2) mutant was isolated from ion beam‐irradiated rice of 1116 lines. The flo11‐2 mutant showed significantly higher chalkiness than the wild type grown under a mean temperature of 28°C, but similar levels of chalkiness to the wild type grown under a mean temperature of 24°C. Whole‐exome sequencing of the flo11‐2 mutant showed three causal gene candidates, including Os12g0244100, which encodes the plastid‐localized 70‐kDa heat shock protein 2 (cpHSP70‐2). The cpHSP70‐2 of the flo11‐2 mutant has an amino acid substitution on the 259th aspartic acid with valine (D259V) in the conserved Motif 5 of the ATPase domain. Transgenic flo11‐2 mutants that express the wild‐type cpHSP70‐2 showed significantly lower chalkiness than the flo11‐2 mutant. Moreover, the accumulation level of cpHSP70‐2 was negatively correlated with the chalky ratio, indicating that cpHSP70‐2 is a causal gene for the chalkiness of the flo11‐2 mutant. The intrinsic ATPase activity of recombinant cpHSP70‐2 was lower by 23% at Vmax for the flo11‐2 mutant than for the wild type. The growth of DnaK‐defective Escherichia coli cells complemented with DnaK with the D201V mutation (equivalent to the D259V mutation) was severely reduced at 37°C, but not in the wild‐type DnaK. The results indicate that the lowered cpHSP70‐2 function is involved with the chalkiness of the flo11‐2 mutant.

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Section: Mechanisms Underlying the Induction Of Rice Seed Chalkiness mentioning

confidence: 99%

FLOURY ENDOSPERM11‐2 encodes plastid HSP70‐2 involved with the temperature‐dependent chalkiness of rice (Oryza sativa L.) grains

et al. 2020

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“…High temperature during grain filling reduces the grain quality of rice (Oryza sativa L.); in particular, it results in chalky grains, whose direct cause is proposed to be the imbalance of starch biosynthesis and degradation (Mitsui, Yamakawa, & Kobata, 2016). High temperature downregulates the expression of starch biosynthesis genes (Yamakawa, Hirose, Kuroda, & Yamaguchi, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of high temperature and shading on grain abscisic acid content and grain filling pattern in rice (Oryza sativaL.)

Tsukaguchi

Tanaka

Inoue

et al. 2018

Plant Production Science

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Abscisic acid (ABA) is a key factor regulating starch biosynthesis genes and is involved in assimilate partitioning to individual spikelets. The objective of this study was to clarify the effects of high temperature and shading during grain filling on grain ABA content and the grain filling pattern of spikelets located at different positions in a panicle. We grew the rice cultivar 'Koshihikari' in pots in 2009 under two temperature regimes and two light conditions during grain filling. We periodically measured grain dry weight and grain ABA content (pmol per grain) and concentration (pmol per grain dry weight). Shading increased a grain weight difference between superior and inferior spikelets while high temperature decreased the difference regardless of light condition. High temperature decreased ABA content and concentration in grains. There was a close correlation between mean grain ABA content and mean grain-filling rate averaged over the first half of grain filling. ARTICLE HISTORY

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“…Maturation of storage proteins generates H 2 O 2 as a byproduct, which is not only one of the toxic reactive oxygen species (ROS) but an important signaling molecule in the regulation of a variety of processes including heat tolerance (Mitsui et al, 2016). Improving plant nitrogen status lowers the canopy temperature through increased transpiration (Xiong et al, 2015); however, how the plant's nitrogen status before heading affects BBWG is unclear.…”

Section: Discussionmentioning

confidence: 99%

The effects of nitrogen uptake before and after heading on grain protein content and the occurrence of basal- and back-white grains in rice (Oryza sativa L.)

Tsukaguchi

Taniguchi

Ito

2016

Plant Production Science

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Molecular physiological aspects of chalking mechanism in rice grains under high-temperature stress

Cited by 45 publications

References 65 publications

FLOURY ENDOSPERM11‐2 encodes plastid HSP70‐2 involved with the temperature‐dependent chalkiness of rice (Oryza sativa L.) grains

FLOURY ENDOSPERM11‐2 encodes plastid HSP70‐2 involved with the temperature‐dependent chalkiness of rice (Oryza sativa L.) grains

Effects of high temperature and shading on grain abscisic acid content and grain filling pattern in rice (Oryza sativaL.)

The effects of nitrogen uptake before and after heading on grain protein content and the occurrence of basal- and back-white grains in rice (Oryza sativa L.)

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