Molecular Prevalence of Anaplasma Marginale in Cattle and Identification of Rhipicephalus, Boophilus, Hyalomma, And Amblyomma Ticks from District Mirpurkhas and Hyderabad

Abstract

Introduction: Anaplasma marginal is a rickettsial organism and is the causative agent in cattle anaplasmosis. This Parasite attacks the red blood cells after infection of sensitive bovine and is transmitted by ticks. Methodology: The study was aimed to detect Anaplasma marginale through PCR the prevalence of four genera (Rhipicephalus, Boophilus, Hyalomma, and Amblyomma) of ticks, twelve hundred animals were observed, 560 samples of blood randomly taken from cattle that possesses ticks on their body and these samples were shifted to the Laboratory of Parasitology, Department of Veterinary Parasitology, Sindh Agriculture University, Tandojam. The infected cattle exhibited clinical signs such as Temperature, decrease in appetite, nausea, and chills. The study was conducted over a period of one year from November 2020 to November 2021. Results: The results of the study showed a high rate of Anaplasma infection (88.33%) and a prevalence of ticks at Mirpurkhas. Month-wise data reveals that the maximum infection was shown in the month of June and the minimum in the month of November at District Mirpurkhas, while at Hyderabad, the month-wise data indicates the Maximum infection rate in the month of May, and the minimum infection was found in the month of November. Conclusion: Ticks collected from two districts of Mirpurkhas and Hyderabad proceeded for identification of Tick’s genus, and the mostly ticks which were infested on animals concerned with the Hyalomma genus (26.11%) Amblyomma genus (25.37%), Boophilus genus (24.62%), and Rhipicephalus genus (23.88%).

Investigating The Molecular Aspects of Theileria Annulata In Naturally Infected Animals, Alongside A Mention of Tick Distribution In Hyderabad And Karachi

“…PCR Product Analysis: Subsequently, the PCR product obtained from these cycles underwent electrophoresis for further analysis or characterization. [13].…”