Molecular prevalence of Blastocystis sp. and subtype diversity in fecal samples collected from cattle in dairy farms in Turkey

Öner, Tülay Öncü; Karakavuk, Muhammet; Döşkaya, Aysu Değirmenci; Güvendi, Mervenur; Gül, Aytül; Köseoğlu, Ahmet Efe; Alak, Sedef Erkunt; Gürüz, Adnan Yüksel; Ün, Cemal; Döşkaya, Mert; Can, Hüseyin

doi:10.1016/j.cimid.2022.101850

Cited by 5 publications

(1 citation statement)

References 51 publications

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“…It was first described in 1912 from humans and was named as Blastocystis hominis, but later on its name was changed to Blastocystis spp. [2], since its presence in human, the parasite has been detected habitat within the intestinal tract of wide range of hosts including various animal species, ruminants, rodents, and birds [3], which can also live in the intestine of reptile, fish and even invertebrates like cockroaches [4]. Blastocystis is a unicellular eukaryote parasite belonging to the class Blastocystea [5].…”

Section: Introductionmentioning

confidence: 99%

First evidence of Blastocystis spp. in livestock animals: An emerging zoonosis from Sulaymaniyah Province

Abdullah

2023

JZS

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Blastocystosis is an infection with zoonotic parasite Blastocystis spp. commonly habitat the intestinal tract of wide range of hosts including human, animals and birds. The study conducted during June to November 2022 in Sulaymaniyah province for detection of Blastocystis spp. from livestock animals. For this purpose, a total of 250 fecal samples were collected randomly from cattle, sheep, and goats of different age groups. Based on microscopic examination of the examined fecal smears the overall prevalence rate of Blastocystis spp. was 24.4% among examined ruminant hosts. Higher prevalence rate has been reported from cattle 29% followed by sheep 27%, and goats 16%, with no significant differences. The study data confirmed Blastocystis colonization of livestock animals in the study area, further study for detection of various subtypes by applying of molecular techniques is essential to find out the infected subtypes and define their impact on public health.

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Section: Introductionmentioning

confidence: 99%

First evidence of Blastocystis spp. in livestock animals: An emerging zoonosis from Sulaymaniyah Province

Abdullah

2023

JZS

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Prevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of Blastocystis spp. in Anatolian Water Buffalo (Bubalus bubalis) in Van, Türkiye

Ayan,

Aslan Celik,

Celik

et al. 2024

Veterinary Medicine & Sci

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BackgroundBlastocystis spp. is one of the most common protozoa worldwide, living in the gastrointestinal tract of humans and many other animals. On the basis of the genetic heterogeneity of small subunit ribosomal RNA, at least 28 subtypes (ST1‐ST17, ST21 and ST23–ST32) are reported to exist in mammals and birds.ObjectivesThis study was carried out to determine the prevalence and subtypes of Blastocystis spp. in Anatolian buffaloes (Bubalus bubalis) in Van province in the Eastern Anatolia Region of Turkey.MethodsDNA was extracted using commercial GeneMATRIX Stool DNA Purification Kit and then stored at −20°C until PCR amplification. After PCR amplification of the SSU rRNA gene region positive Blastocystis spp., amplicons from buffalo faeces were sequenced and then deposited in GenBank (OR576949.1, OR576950.1, OR576970.1, OR576971.1, OR577019.1, PP837943.1, PP837940.1, PP837939.1, PP837604.1, PP837937.1, PP837934.1, PP837601.1, PP837936.1 and PP837603.1).ResultsPCR analysis of 120 faecal samples showed a total prevalence of 11.67% (14/120). The prevalence was higher in females and young animals (p > 0.05). Sequence analysis revealed Blastocystis spp., ST10, ST14, ST25 and ST26 subtypes. To our knowledge, Blastocystis subtypes ST25 and ST26 in buffaloes were reported for the first time in this study.ConclusionIt is thought that more large‐scale studies should be carried out to determine the zoonotic subtype potential of this protozoan in the region.

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Genetic characteristics of Blastocystis sp. in cattle from Hebei Province, China

Sun,

Nan,

Zhang

et al. 2024

Microbial Pathogenesis

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Molecular prevalence of Blastocystis sp. and subtype diversity in fecal samples collected from cattle in dairy farms in Turkey

Cited by 5 publications

References 51 publications

First evidence of Blastocystis spp. in livestock animals: An emerging zoonosis from Sulaymaniyah Province

First evidence of Blastocystis spp. in livestock animals: An emerging zoonosis from Sulaymaniyah Province

Prevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of Blastocystis spp. in Anatolian Water Buffalo (Bubalus bubalis) in Van, Türkiye

Genetic characteristics of Blastocystis sp. in cattle from Hebei Province, China

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