Molecular Principles of Adhesion and Biofilm Formation

Kreth, Jens; Herzberg, Mark C.

doi:10.1007/978-3-662-47415-0_2

Cited by 19 publications

(19 citation statements)

References 168 publications

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“…The cell structures responsible for adhesion contribute to the colonisation of the surface of the mucin layer of the gastrointestinal tract, and the subsequent formation of a stable biofilm [54]. Biofilms protect bacteria from host immune factors and antagonistic factors of other members of the microbiota, which ensures close contact of bacteria with each other and with host cells.…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

et al. 2020

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Whole-genome DNA sequencing of Lactobacillus D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both strains belong to the Lactobacillus helveticus, but not to the L. acidophilus, as previously assumed. In addition, 31 exopolysaccharide (EPS) production genes (nine of which form a single genetic cluster), 13 adhesion genes, 38 milk protein and 11 milk sugar utilization genes, 13 genes for and against specific antagonistic activity, eight antibiotic resistance genes, and also three CRISPR blocks and eight Cas I-B system genes were identified in the genomes of both strains. The expression of bacteriocin helveticin J genes was confirmed. In fact, the presence of identified genes suggests that L. helveticus D75 and D76 are able to form biofilms on the outer mucin layer, inhibit the growth of pathogens and pathobionts, utilize milk substrates with the formation of digestible milk sugars and bioactive peptides, resist bacteriophages, show some genome-determined resistance to antibiotics, and stimulate the host’s immune system. Pathogenicity genes have not been identified. The study results confirm the safety and high probiotic potential of the strains.

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Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

et al. 2020

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“…The cell structures responsible for adhesion contribute to the colonisation of the surface of the mucin layer of the gastrointestinal tract, and the subsequent formation of a stable biofilm [70]. Biofilms protect bacteria from host immune factors and antagonistic factors of other members of the microbiota, ensuring close contact of bacteria with each other and with host cells.…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing of Lactobacillus Helveticus D75 and D76 Confirms Safety and Probiotic Potential

Toropov¹,

Demyanova²,

Шалаева³

et al. 2019

Preprint

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Whole-genome DNA sequencing of Lactobacillus D75 and D76 strains (Vitaflor, Russia) was performed using the PacBio RS II platform, followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both strains belong to the Lactobacillus helveticus, but not the L. acidophilus as previously assumed. 31 exopolysaccharide (EPS) production genes (nine of which form a single genetic cluster), 13 adhesion genes, 38 milk protein and 11 milk sugar utilization genes, 13 genes for and against specific antagonistic activity, aight antibiotic resistance genes, and also three CRISPR blocks and eight Cas I-B system genes were identified in the genomes of the both strains. The expression of some genes was confirmed. In fact, the presence of identified genes suggests that L. helveticus D75 and D76 are able to form biofilms on the outer mucin layer, inhibit the growth of pathogens and pathobionts, utilize milk substrates with the formation of digestible milk sugars and bioactive peptides, resist bacteriophages and show some genome-determined resistance to antibiotics, stimulate the host’s immune system. Pathogenicity genes have not been identified. The study results confirm the safety and high probiotic potential of the strains.

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“…Oral biofilm formation begins with the attachment of S. sanguinis and other pioneer colonizers to macromolecular complexes formed on saliva-coated tooth surfaces Kreth & Herzberg, 2015). Negatively charged residues and electrostatic interactions with hydrophilic regions in salivary proteins facilitate their attachment to the tooth surface (Lamkin & Oppenheim, 1993;Lindh, 2002) forming what is referred to as the acquired enamel pellicle (AEP).…”

Section: Initial Attachment and Biofilm Developmentmentioning

confidence: 99%

The road less traveled – defining molecular commensalism with Streptococcus sanguinis

Kreth

Giacaman

Raghavan

et al. 2016

Molecular Oral Microbiology

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Summary The commensal oral microbial flora has evolved with the human host to support colonization of the various intraoral sites without triggering a significant immune response. In exchange, the commensal microbes provide critical protection against invading pathogens. The intrinsic ability of the oral flora to create a symbiotic microbial community with the host can be disturbed, selecting for the overgrowth of a dysbiotic community that can result in dental diseases, such as caries and periodontitis. While the mechanisms of molecular pathogenesis in oral diseases are well characterized, much less is known about the molecular mechanisms used by the commensal flora to maintain oral health. Here we focus on the commensal species Streptococcus sanguinis, which is found in abundance in the early oral biofilm and is strongly correlated with oral health. S. sanguinis exhibits a variety of features that make it ideally suited as a model organism to explore the molecular basis for commensalism. As such, this review will describe our current mechanistic understanding of S. sanguinis commensalism and speculate upon its molecular traits that may be exploitable to maintain or restore oral health under conditions that would otherwise lead to disease.

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Molecular Principles of Adhesion and Biofilm Formation

Cited by 19 publications

References 168 publications

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

Whole-Genome Sequencing of Lactobacillus Helveticus D75 and D76 Confirms Safety and Probiotic Potential

The road less traveled – defining molecular commensalism with Streptococcus sanguinis

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