Molecular profile of specific IgE to allergenic components in allergic adults using allergen nano‐bead array

Abstract

Background An increasing interest in the field of molecular diagnosis of allergy has been developed in recent years and it goes to be as the routine in vitro protocol in allergy diagnosis. friendly allergen nano‐bead array (FABER) is a new multiplex assay for the evaluation of specific IgE against 244 allergens including whole extracts and allergenic molecules. The research intended to assess the pattern of IgE sensitization to allergenic components of allergens in allergic adults using FABER 244. Methods Sixty patients with allergic diseases entered this cross‐sectional study. Specific IgE to 122 whole allergens extracts and 122 allergenic components were assessed using an allergen nano‐bead array (FABER) for all patients. This test includes inhalant and food allergens. Results Thirty‐seven patients were male (61.7%). The mean (SD) age of patients was 30.73(±6.87) years. As the allergen nano‐bead array results showed, Lolium perenne (63.3%), Phleum pratense (60%) and Platanus acerifolia (51.7%) were considered as the most common IgE sensitizations to the aeroallergen extracts. Moreover, Lol p 1, Phl p 1.0102 and Cup a 1 were found as the most frequent allergenic components in our allergic patients. Among protein families, CCD‐bearing proteins, expansin, cysteine protease and profilin families illustrated the highest allergic sensitization. Conclusions The results of the present study demonstrated that despite the higher prevalence of sensitization to Salsola kali (47.2%) using extract‐based assays in the previous phase of this research, allergenic components of grasses (Lol p 1, Phl p 1.0102), Cup a 1 as well as Sal k1 as the major components of Cupressuss arizonica and Salsola kali showed the higher sensitization, respectively, in adults’ allergic patients using FABER test.

Understanding the heterogeneity of childhood allergic sensitization and its relationship with asthma

“…The Task Force recently published The Molecular Allergology User's Guide 2.0, which provides comprehensive guidance on CRD for clinicians, scientists and interested readers [54 ▪▪ ]. Technological developments in molecular diagnostics have led to products in which sIgE responses to hundreds of allergen components from large number of different sources can be measured simultaneously using multiplex-based specific IgE antibody assays [55,56] (reviewed in [57 ▪ ,58 ▪ ]).…”

The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali)

“…Pollen from weeds, in particular from the plant families of Asteraceae, Amaranthaceae, Plantaginaceae, Urticaceae , and Euphorbiaceae , represents an important allergen source worldwide ( 1 ). In particular, the family of Amaranthaceae comprising three major genera, Chenopodium, Amaranthus , and Salsola , is considered to be highly relevant in Middle Asia, southern Europe, western United States, Saudi Arabia, Kuwait, and Iran ( 2 – 5 ). Russian thistle, also termed saltwort ( Salsola kali ), is a widely distributed weed and was discovered as an important allergenic plant in the United States already in 1933 ( 6 ).…”