Molecular profiling and functional delineation of peroxiredoxin 3 (HaPrx3) from the big-belly seahorses (Hippocampus abdominalis) and understanding their immunological responses

Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication

“…Moreover, MFO assays showed that the r CiPrx3 protein could inhibit DNA damage in a dose-dependent manner, further indicating the antioxidant activity of CiPrx3 . Similar results of Prxs-mediated inhibition of DNA damage caused by the MFO system were also reported in other fish species [ 5 , 48 ]. Interestingly, we also found that the E. coli BL21 strains with CiPrx3 also showed resistance to heavy metal toxicity, which illustrated the important role of CiPrx3 not only in the host response to oxidative stress, but also in the response to heavy metal toxicity.…”

“…Therefore, the higher expression level of CiPrx3 in the liver and muscle may be beneficial for reducing the accumulation of ROS. Highly expressed levels of Prx3 in liver or muscle tissues were also observed in other fish, such as medaka [ 15 ], gilthead sea bream [ 45 ], rock bream [ 16 ], and large-belly seahorses [ 5 ], implying the conserved role of Prx3.…”

“…By scavenging reactive oxygen species (ROS), Prxs protect cells from oxidative damage [ 1 ]. Prxs are ubiquitously conserved in a wide variety of living organisms ranging from prokaryotes to eukaryotes [ 2 , 3 , 4 , 5 ]. The Prxs family can be classified into six subtypes based on their structure and catalytic domains: the typical 2-Cys type (Prx1, Prx2, Prx3, and Prx4), the atypical 2-Cys type (Prx5), and the 1-Cys type (Prx6) [ 6 , 7 ].…”

“…Prx3 is an indispensable ROS scavenger that protects tumor cells against oxidative damage and subsequent apoptosis [ 14 ]. To date, the Prx3 gene has been cloned from several fish species, including medaka [ 15 ], rock bream [ 16 ], common carp [ 17 ], and big-belly seahorses [ 5 ]. However, the functions of Prx3 in teleost fish, especially the function in host immune response and defense against pathogens, are still unknown.…”

Molecular characterization and functional analysis of peroxiredoxin 3 (NdPrx3) from Neocaridina denticulata sinensis

“…The DNA protection activity of Prxs was investigated in Hippocampus abdominalis and Oplegnathus fasciatus [ 23 , 24 ]. Previous studies of Prx3 have been reported in many aquatic organisms, including O. fasciatus [25] , H. abdominalis [26] , and Cyprinus carpio [27] . However, little is known about Prx3 in Neocaridina denticulata sinensis .…”