Molecular Profiling Identifies Prognostic Subgroups of Pediatric Glioblastoma and Shows Increased YB-1 Expression in Tumors

Abstract

Our work, the first study of gene expression profiles in pGBM, provides valuable insight into active pathways and targets in a cancer with minimal survival, and suggests that these tumors cannot be understood exclusively through studies of aGBM.

Impact of host ageing on the metastatic phenotype

“…On the other hand, cancer cells are thought to acquire metastatic phenotype as a result of selective pressures operative in various target organs, effects that favor clones harbouring certain cellular properties (and mutations) over the other (Fidler 2003;Chiang and Massague 2008;Wu et al 2012). Since the molecular make up of morphologically similar tumors is not identical in various age groups (Faury et al 2007), it is tempting to ask whether (and to what extent) ageing processes also intersect with mechanisms of mutational variability and host-dependent selection of metastatic cancer cell variants (Nowell 1976).…”

“…We believe that this is an important and underdeveloped area of investigation. Inasmuch as histologically similar, adult and pediatric malignancies are molecularly different (Faury et al 2007), so too could be the biology and therapeutic dimensions of the disseminated disease. This is important, as present preclinical drug development efforts, while mainly directed at disseminated cancers, rarely (if ever) use aged mice as model systems (Francia et al 2011).…”

Pharmacogenomic approach for the identification of novel determinants of acquired resistance to oxaliplatin in colorectal cancer

“…However, the quality of gene expression data obtained from microarrays can vary greatly in platform and procedures used. Quantitative RT-PCR is a commonly used validation tool for confirming gene expression results obtained from microarray analysis and is often referred to as the ''gold standard'' for gene expression measurements due to its advantages in detection sensitivity, sequence specificity, large dynamic range, as well as its high precision and reproducible quantification compared with other techniques (26,27).…”

Les glioblastomes de l’enfant et du jeune adulte

“…Les caractéristiques histologiques des GBM sont identiques quel que soit l'âge du patient ou la localisation de la tumeur, mais leurs caractéristiques génétiques diffèrent [1][2][3][4]. Par exemple, chez l'enfant, moins de 10 % des tumeurs ont des mutations/amplifications du récepteur à activité tyrosine kinase de l'EGF (epidermal growth factor) (EGFR), ou des mutations des enzymes isocitrate déshydroxygénase 1 ou 2 (IDH1/2), qui caractérisent respectivement la majorité …”