Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Endo, Akihito; Okada, Sanae

doi:10.2323/jgam.53.191

Cited by 36 publications

(33 citation statements)

References 40 publications

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“…[15], Endo et al . [4], and Morita et al . [13, 14] isolated lactobacilli from horse fecal samples, including Thoroughbreds.…”

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confidence: 99%

“…We also isolated Bifidobacterium boum from the intestinal flora of Thoroughbreds [3]. Based on the above studies, a horse probiotics consisting of Lactobacillus ruminis KK14, L. equi KK15, L. reuteri KK18, L. johnsonii KK21 [13], and B. boum HU [4] was prepared and termed LacFi TM . LacFi TM has been recognized as “Generally Recognized As Safe (GRAS)” following use for >11 years.…”

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confidence: 99%

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Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds

Tanabe

Suzuki

Wasano

et al. 2014

JES

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We previously isolated the commensal bacteria lactobacilli and bifidobacteria from the Thoroughbred intestine and prepared the horse probiotics LacFiTM, consisting of Lactobacillus ruminis KK14, L. equi KK 15, L. reuteri KK18, L. johnsonii KK21, and Bifidobacterium boum HU. Here, we found that the five LacFiTM constituent strains remarkably suppressed pro-inflammatory interleukin-17 production in mouse splenocytes stimulated with interleukin-6 and transforming growth factor-β. The protective effects of the probiotic on impaired intestinal barrier function were evaluated in Caco-2 cells treated with tumor necrosis factor-α. Evaluation of transepithelial resistance showed that all the strains exhibited intestinal barrier protective activity, with significant suppression of barrier impairment by L. reuteri KK18. The LacFiTM constituent strains were detected in neonatal LacFiTM-administered Thoroughbred feces using polymerase chain reaction denaturing gradient gel electrophoresis and culture methods. These five strains were found to be the predominant lactobacilli and bifidobacteria in the intestinal microbiota of LacFiTM-administered Thoroughbreds. Administration of LacFiTM to neonatal Thoroughbreds decreased diarrhea incidence from 75.9% in the control group (n=29 neonatal Thoroughbreds) to 30.7% in the LacFiTM-administered group (n=101 neonatal Thoroughbreds) immediately after birth to 20 weeks after birth. LacFiTM treatment also prevented diarrhea especially at and around 4 weeks and from 10 to 16 weeks. The duration of diarrhea was also shorter in the probiotics-administered group (7.4 ± 0.8 days) than in the control group (14.0 ± 3.2 days). These results indicate that the LacFiTM probiotics regulates intestinal function and contributes to diarrhea prevention.

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“…[15], Endo et al . [4], and Morita et al . [13, 14] isolated lactobacilli from horse fecal samples, including Thoroughbreds.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds

Tanabe

Suzuki

Wasano

et al. 2014

JES

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“…thermophilus (bands f and g). Even in pure culture, multiple fragments with different migration distances were reportOkada, 2005; Endo et al, 2007;Walter et al, 2001;Zanini et al, 2007). However, our preliminary experiments revealed that L. kefiranofaciens, Leu.…”

Section: Resultsmentioning

confidence: 99%

Denaturing Gradient Gel Electrophoresis Analysis of Lactic Acid Bacteria and Yeasts in Traditional Mongolian Fermented Milk

Miyamoto

Seto

Nakajima

et al. 2010

FSTR

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We analyzed lactic acid bacteria (LAB) and yeast populations in samples of airag, undaa, and hoormog, which are varieties of traditional fermented milk produced in Mongolia. To effectively detect lactobacilli, lactococci/streptococci/enterococci, and leuconostocs for complete analysis of the complex LAB population in airag, we developed two new primer sets and examined these primer sets in three different combinations using denaturing gradient gel electrophoresis (DGGE). DGGE analysis revealed the diversity of the LAB and yeast populations in these traditional fermented milk samples; six to 11 LAB and yeast species were detected. The species common to all samples include Lactobacillus helveticus, Leuconostoc mesenteroides, Lactococcus lactis, and Kluyveromyces marxianus. Lc. raffinolactis, Enterococcus faecium/ hirae/durans/villorum/ratti, Streptococcus thermophilus, L. kefiranofaciens, L. kefiri/buchneri, Leu. citreum, and Leu. lactis were detected in multiple samples, while lactose non-fermenting Saccharomyces cerevisiae was only detected in one sample of hoormog.Keywords: traditional fermented milk, denaturing gradient gel electrophoresis, lactic acid bacteria, yeast *To whom correspondence should be addressed. E-mail: mari-miya@snowbrand.co.jp IntroductionAirag is the most popular traditional fermented milk in Mongolia. It is made using horse milk and has a low alcohol content (normally less than 3%). Similar types of traditional fermented milk are also consumed in Inner Mongolia (chigee and tsege) and Russia (koumiss). Hoormog and undaa, respectively prepared using the milk of camel and cow, are less popular types of Mongolian fermented milk.Airag is generally fermented by adding fresh raw milk to a small quantity of previously prepared airag, and the mixture is then stirred several thousand times. At the end of the horse lactation period, Mongolian people prepare hurunge, naturally dried airag which is stored for use as a starter culture in the following summer.

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“…According to a previous report, quantitative real-time polymerase chain reaction (PCR) using Light Cycler 480 SYBR Green I Master (Roche Diagnostics K.K., Japan) was performed with 16S rRNA gene-targeted, group-specific primers for all bacteria, Bacteroides, Prevotella, Lactobacillus, Streptococcus, Clostridium coccoides, Clostridium leptum and Bifidobacterium [16]. The PCR primers used in the present study were the same as those previously reported [17]- [19]. The relative ratio of each test bacteria to all bacteria (%) was calculated.…”

Section: S Rrna Gene Sequence Analysis For Intestinal Floramentioning

confidence: 99%

The Mechanism of Decreased Serum Phosphorus Levels in Rats with Chronic Kidney Disease after Oral Administration of <i>Bifidobacterium longum</i>

Nagano¹,

Futaya²,

Kohno³

et al. 2015

AiM

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Chronic kidney disease (CKD) patients are prone to disturbances in the intestinal microbiota, which contributes to CKD progression and complications. We previously reported a reduction of serum phosphorus (P) levels in hemodialysis patients receiving oral encapsulated bifidobacteria. The present study was conducted to clarify the mechanisms of P-lowering effect of bifidobacteria on CKD rats. CKD was induced in rats by 5/6 nephrectomy. Five weeks later, the rats were fed for 4 weeks on a powder diet containing encapsulated bifidobacteria. At the end of the study, intestinal contents were sampled for analyses of pH, intestinal flora and short-chain fatty acids (SCFAs). Oral administration of bifidobacteria halted the onset and progression of hyperphosphatemia in CKD rats. The increased number of bifidobacteria was confirmed in the cecum. In addition, the increase in intestinal pH in CKD rats was decreased after bifidobacteria treatment, along with increases in some SCFAs. Furthermore, positive correlation between serum P levels and intestinal pH was observed. In conclusion, the mechanism for the P-lowering effect of bifidobacteria was supposed as follows: CKD conditions increase aerobic bacteria which hydrolyze urea into ammonia. Elevated pH decreases ionization of intestinal calcium (Ca) which leads to an increase in free phosphate ions through reduction of Ca phosphate crystal precipitation. Administered bifidobacteria fer-* Corresponding author. N. Nagano et al. 532mented carbohydrates to produce SCFAs, resulting in acidification of the intestinal lumen. The resulting low intestinal pH increases Ca ionization, which binds with free phosphate ions as an intrinsic P binder, resulting in the reduction of serum P levels.

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Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Cited by 36 publications

References 40 publications

Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds

Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds

Denaturing Gradient Gel Electrophoresis Analysis of Lactic Acid Bacteria and Yeasts in Traditional Mongolian Fermented Milk

The Mechanism of Decreased Serum Phosphorus Levels in Rats with Chronic Kidney Disease after Oral Administration of <i>Bifidobacterium longum</i>

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Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Cited by 36 publications

References 40 publications

Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds

Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds

Denaturing Gradient Gel Electrophoresis Analysis of Lactic Acid Bacteria and Yeasts in Traditional Mongolian Fermented Milk

The Mechanism of Decreased Serum Phosphorus Levels in Rats with Chronic Kidney Disease after Oral Administration of &lt;i&gt;Bifidobacterium longum&lt;/i&gt;

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The Mechanism of Decreased Serum Phosphorus Levels in Rats with Chronic Kidney Disease after Oral Administration of <i>Bifidobacterium longum</i>