Molecular profiling of microbial community structure and their CAZymes via metagenomics, from Tsomgo lake in the Eastern Himalayas

Rai, Aditi; Bhattacharjee, Arindam

doi:10.1007/s00203-021-02278-7

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…The CAZymes can synthesize a variety of enzymes, such as chitinase, glucanase, cellulase, xylanase, pectinase, protease, etc. Not only do these enzymes help to control the growth and reproduction of bacteria that are antagonistic toward pathogens, but they are also essential for the competition between organisms in terms of nutrients and ecological sites [47].…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Bacillus velezensis with Antibacterial Activity and Its Inhibitory Effect on Gray Mold Germ

Wang

Liang

et al. 2023

Agronomy

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The present study provides a comprehensive overview of the Bacillus velezensis strain Htq6, and its potential applications in plant disease control. Htq6 is an endophytic bacterium derived from walnut, which was found to possess a strong inhibitory effect on a wide range of plant pathogenic microorganisms and was identified as a good plant disease control agent. The entire genome of the Bacillus velezensis Htq6 was sequenced, and a comparative genomic analysis was conducted with various Bacillus species in order to better understand the mechanism of the strain’s biological control. At the same time, a new classification result was presented. Additionally, transcriptome analysis was performed to explore the response mechanism of tomato gray mold fungus after treatment with the fermentation liquid of Bacillus velezensis Htq6. The study analyzed the distribution of various secondary metabolite gene clusters in the Bacillus model strains and employed RNA-Seq technology to obtain transcriptome expression profiles. Furthermore, the cell wall, cell membrane, and antioxidant-related genes of Botrytis cinerea were analyzed, providing insight into the antibacterial mechanism of biocontrol bacteria and the stress response mechanism of Botrytis cinerea. The results of the research are promising, and could potentially lead to the development of an effective biocontrol agent for the prevention and control of various plant diseases.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Bacillus velezensis with Antibacterial Activity and Its Inhibitory Effect on Gray Mold Germ

Wang

Liang

et al. 2023

Agronomy

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“…The importance of local environmental factors has been highlighted in studies on microbial community composition of several regions such as remote high-altitude lakes of Mt. Everest region, Tsomgo lake in the Eastern Himalayas and Gangotri soil in Central Himalayas [39][40][41]. From these studies it is evident that biogeography patterns are exhibited by microbial communities.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic diversity of microbial communities and physicochemical properties along a vertical soil section of Lam Pokhari Lake in Eastern Himalayas.

Sinha

Ankulkar²,

Kandekar³

et al. 2022

Preprint

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It is well documented that the microbes found in soil are key drivers of various environmental processes such as nutrient cycling, soil formation and production of key enzymes and metabolites. There is a possibility of infinite number of microbes being buried in the layers of soil that formed over timescales of hundreds and thousands of years. However, there are few studies till date which correlate the depth and physicochemical properties of soil with the microbial population inhabiting that soil. In this study, an investigation of eleven samples along a 100 cm vertical section of soil from Lam Pokhari Lake, Eastern Himalayas, India was done by Sanger sequencing and Field emission scanning electron microscope-energy dispersive X-ray spectroscopy (FESEM-EDS). Further, the distribution of culturable bacteria and fungi was studied. Bacteria belonging to four different phyla- Firmicutes, Actinobacteria, Beta-proteobacteria and Gamma-proteobacteria were present in the soil samples as inferred from 16s rDNA gene sequencing. Internal transcribed spacer (ITS) gene sequencing revealed the presence of fungi belonging to Ascomycota and Basidiomycota. Lastly, the particle size was obtained and the homogeneity of the samples was assessed by FESEM and imaging. EDS was used for obtaining the composition of seventeen elements in the soil samples. The weight percentage of oxygen and silicon was the highest in all the samples. Each sample showed a characteristic microbial distribution, particle size and elemental profile, which validated the use of a multidisciplinary approach to study soil samples.

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“…Chauhan [ 56 ] and Dhanjal, Chopra [ 57 ] reviewed several novel enzymes discovered using a metagenomic approach, including lipases and esterases obtained from different environmental samples. Recently, a cold-active esterase PMGL3 was obtained from the metagenomic DNA library of Siberian permafrost, and other lipase genes have also been isolated and identified from various metagenomic libraries [ 36 , 58 , 59 ]. These studies showed that metagenomics is an effective technique for identifying, extracting, and discovering novel lipolytic enzymes.…”

Section: Approaches For Isolation Of Genes Encoding Cold-active Lipas...mentioning

confidence: 99%

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues

Matinja

Leow

Oslan

et al. 2022

IJMS

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Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase.

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Molecular profiling of microbial community structure and their CAZymes via metagenomics, from Tsomgo lake in the Eastern Himalayas

Cited by 10 publications

References 33 publications

Characterization of a Bacillus velezensis with Antibacterial Activity and Its Inhibitory Effect on Gray Mold Germ

Characterization of a Bacillus velezensis with Antibacterial Activity and Its Inhibitory Effect on Gray Mold Germ

Phylogenetic diversity of microbial communities and physicochemical properties along a vertical soil section of Lam Pokhari Lake in Eastern Himalayas.

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues

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