Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

Das, Nandana; Kumar, T. Rajendra

doi:10.1530/jme-17-0308

Cited by 116 publications

(84 citation statements)

References 291 publications

(289 reference statements)

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“…In adult females, functional FSH receptors (FSHRs) located on granulosa cells respond to FSH, leading to follicle maturation. Similar to other pituitary and placental hormones, FSH consists of two non-covalently associated heterodimeric α-and β-subunits, of which the β-subunit is hormone-specific and confers biological activity (Hunzicker-Dunn & Mayo 2015, Das & Kumar 2018. FSH signaling is regulated in part via N-glycosylation.…”

Section: Introductionmentioning

confidence: 99%

Recombinant FSH glycoforms are bioactive in mouse preantral ovarian follicles

et al. 2019

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Female reproductive aging is characterized by a rise in follicle-stimulating hormone (FSH) levels during peri-menopause. N-linked glycans are co-translationally attached to the Asn7 and Asn24 residues on the FSHβ subunit. Differences in the number of N-glycans on the FSHβ subunit result in distinct glycoforms: hypo-glycosylated (FSH21/18, glycans absent on either Asn24 or Asn7, respectively) or fully-glycosylated (FSH24, glycans present on both Asn7 and Asn24). The relative abundance of FSH glycoforms changes with advanced reproductive age, shifting from predominantly FSH21/18 in younger women to FSH24 in older women. Previous in vitro studies in granulosa cell lines and in vivo studies using Fshb-null mice showed these glycoforms elicit differential bioactivities. However, the direct effects of FSH glycoforms on the mouse ovarian follicle have not yet been determined. In this study, we isolated secondary follicles from pre-pubertal mice and treated them with 20- or 100 ng/mL purified recombinant FSH glycoforms for 1 h or 18–20 h. Analysis of phosphorylated PKA substrates showed that glycoforms were bioactive in follicles following 1-h treatment, although differential bioactivity was only observed with the 100 ng/mL dose. Treatment of follicles with 100 ng/mL of each glycoform also induced distinct expression patterns of FSH-responsive genes as assessed by qPCR, consistent with differential function. Our results, therefore, indicate that FSH glycoforms are bioactive in isolated murine follicles.

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Section: Introductionmentioning

confidence: 99%

Recombinant FSH glycoforms are bioactive in mouse preantral ovarian follicles

et al. 2019

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“…FSH has a longer half-life than LH15. It was found that FSH has a complex secretion regulation due to various molecular factors that influence it, such as adiponectin, synaptotagmin 9 (syt-9) and ACTH [16]. We also found that both serum ferritin and transferrin saturation have negative correlations with testosterone levels, although this correlation was not statistically significant (r =-0.021, p = 0.991 and r =-0.016, and p = 0.933, respectively).…”

Section: Correlation Between Serum Ferritin and Transferrin Saturatiomentioning

confidence: 69%

Correlation Between Serum Ferritin, Transferrin Saturation and Pituitary Mri T2 Relaxation Times and Fsh, Lh and Testosterone Levels in Male Transfusion-Dependent Thalassemia Patients

et al. 2020

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Objective: The purpose of this study is to see the correlation between iron overload with the hypogonadal state by analyzing the correlation between ferritin serum, transferrin saturation and pituitary MRI T2 relaxation time with FSH, LH and testosterone levels. Methods: This is a cross-sectional study of 32 male subjects with transfusion-dependent thalassemia. The subjects were collected with a consecutive sampling technique in the thalassemia outpatient clinic in National Hospital in Indonesia. Measurements of serum ferritin, transferrin saturation, FSH, LH and testosterone were taken using ELISA technique. Pituitary MRI T2 relaxation time was done using MRI Avanto 1.5 Tesla. Results: In this study, secondary sexual characteristics were not fully achieved in 62.5% of patients. Low testosterone levels were found in 25% of patients. There was a negative correlation between transferrin saturation and pituitary MRI T2 relaxation time in the normal testosterone level group. Conclusion: This study showed a high rate of patients who had not achieve puberty, but a low rate of patients with low testosterone, which means there is a weak negative correlation between transferrin saturation and pituitary MRI T2 relaxation times.

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“…Several factors such as testis steroids, inhibin, activin and folistatin are that exert feedback on FSH. These factors regulate FSH concentration due of central effects on Gonadotropin-releasing hormone, and possible absence of any significant alteration in FSH level is the result of modifying effects of these factors [37].…”

Section: Discussionmentioning

confidence: 99%

Probiotic effects on sperm parameters, oxidative stress index, inflammatory factors and sex hormones in infertile men

et al. 2020

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Background: Impaired sperm production, decreased sperm motility, and increased oxidative stress levels are the main causes of male infertility. To evaluate effects of supplementation with probiotic on the spermatogram, seminal oxidative stress biomarkers, inflammatory factors and reproductive hormones. Methods: In this randomized, double-blind controlled clinical trial, 52 idiopathic asthenozoospermia men, attending urology clinic, were randomly assigned to one of intervention and placebo (n =26) groups. Participants in the intervention group took daily 500 mg Probiotic and those in the placebo group took daily placebo for 10 wk. Semen parameters, total antioxidant capacity, malondialdehyde, inflammatory factors, reproductive hormones, anthropometric and physical activity assessments were measured at the baseline and at the end of the study. Statistical analysis was performed using SPSS software. Results: Out of 52 who participated in this study, 25 men in the intervention group and 25 men in the control group completed the protocol of the study. After the intervention, ejaculate volume, number, concentration and the percentage of motile sperm, total antioxidant capacity of plasma significantly increased in the intervention group and the concentration of plasma malondialdehyde and inflammatory markers significantly decreased in the intervention group. Conclusion: Probiotic supplementation in infertile men, leading to significant increase in concentration and motility and significant reduce in oxidative stress and inflammatory markers. Possibly oral intake of probiotic has the potential to be one of the ways to deal with oxidative damage sperm of infertile men. activity of antioxidant enzymes and also decrease the content of nitric oxide and malondialdehyde levels[14]. Lactic acid bacteria (LAB) and bifidobacteria are the greatest quantity regularly used probiotics, which has strong antioxidant properties. These metabolic antioxidant activities may be assigned to ROS scavenging, enzyme inhibition, and reduction activity or inhibition of ascorbate autoxidation in the intestine by neutralizing free radicals[15]. Also, several randomized controlled trials have now shown that microbial modification by probiotics may improve gastrointestinal symptoms and inflammation in rheumatoid arthritis, ulcerative colitis, and multiple sclerosis[16]. According to the mentioned issues, the present study designed to evaluate the efficacy of probiotic supplement on sperm quality, oxidative stress index, inflammatory factors and sex hormones in men with idiopathic infertility. Methods Study Design This study designed as a double blind randomized controlled clinical trial that approved with Ethics

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Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

Abstract: 26Follicle-stimulating hormone (FSH) plays fundamental roles in male and female fertility. FSH is 27 a heterodimeric glycoprotein expressed by gonadotrophs in the anterior pituitary. The hormone-28 specific FSHβ-subunit is non-covalently associated with the common α-subunit that is also

Cited by 116 publications

References 291 publications

Recombinant FSH glycoforms are bioactive in mouse preantral ovarian follicles

Recombinant FSH glycoforms are bioactive in mouse preantral ovarian follicles

Correlation Between Serum Ferritin, Transferrin Saturation and Pituitary Mri T2 Relaxation Times and Fsh, Lh and Testosterone Levels in Male Transfusion-Dependent Thalassemia Patients

Probiotic effects on sperm parameters, oxidative stress index, inflammatory factors and sex hormones in infertile men

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