Molecular Regulation of the Metabolic Pathways of the Medicinal Plants:  &amp;lt;i&amp;gt;Phyla dulcis&amp;lt;/i&amp;gt;

Osuji, Godson O.; Weerasooriya, Aruna; Ampim, Peter A. Y.; Carson, Laura; Johnson, Paul M.; Jung, Yoonsung; Duffus, Eustace; Woldesenbet, Sela; South, Sanique; Idan, Edna; Johnson, D A; Clarke, Diadrian; Lawton, Billy; Parks, Alfred L.; Fares, Ali; Johnson, Alton

doi:10.4236/ajps.2015.611171

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Additionally, it is employed as a decoction for the treatment of coughs, colds, bronchitis, asthma, and colic (Moreno-Murillo et al, 2010). Osuji et al (2015) and Kinghorn et al (2011) reported that Lippia dulcis is approximately a thousand times sweeter than sucrose because it contains hernundulcin sesquiterpene. This plant has shown potential for use as a natural low-calorie sweetener in the dietary management of diabetes/ obesity (Kinghorn et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Rocha

Araújo

Assis

et al. 2023

PCC&M

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Lippia dulcis Trevir. (Verbenaceae) is a species with medicinal potential that is used as a tranquilizer and to control diabetes in the western region of Pará, Brazil. The objective of this study was to determine the establishment and clonal propagation of Lippia dulcis through single nodal segments grown in different concentrations of salts in a MS (Murashige and Skoog) medium (MS, MS/2, MS/4) and determine the best period for subculturing the species. Nodal segments cultivated in the MS medium showed a higher survival percentage and better development than those grown in more dilute media (MS/2, MS/4). The concentration of the salts affected the dry weight gain of some plant parts. Plantlets cultivated in the MS medium obtained better leaf dry weight and total leaf area than plantlets cultivated in half-strength MS. The half-strength MS medium proved to be most effective for root length growth and root dry weight gainin vitro. The growth curve showed that day 30 was the time for subculturing the species. Starting with only one nodal segment, 3125 plantlets were obtained after sixth months of cultivation at a multiplication rate of five. The plantlets of Lippia dulcis were removed from the flasks and acclimatized with success in a greenhouse.

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Section: Introductionmentioning

confidence: 99%

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Rocha

Araújo

Assis

et al. 2023

PCC&M

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“…These biochemical considerations give strength to the repeatedly published over whelming preponderance of G+C-rich regions as against the lesser frequency of the A+T-rich regions in the genome[20] [36][37]. GDH hexameric isoenzymes are being applied routinely for the synthesis of nucleic acid hybridization probes for studying the biochemical pathways of the recalcitrant medicinal plants, Phyla dulcis[9] [46]. Studies which applied genetic code-based hybridization probes instead[47] did not knock out the mRNAs encoding the monoterpene synthases, whereas studies with hybridization probes based on GDH-synthesized RNA enzyme have knocked out the mRNAs encoding the monoterpene synthases (Osuji unpublished results).…”

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Structural Properties of the RNA Synthesized by Glutamate Dehydrogenase for the Degradation of Total RNA

Osuji¹,

Johnson²

2018

AER

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Glutamate dehydrogenase (GDH)-synthesized RNA, a nongenetic code-based RNA is suitable for unraveling the structural constraints imposed on the regulation (transcription, translation, siRNA etc.) of metabolism by genetic code. GDH-synthesized RNAs have been induced in whole plants to knock out target mRNA populations thereby producing plant phenotypes that are allergen-free; enriched in fatty acids, essential amino acids, shikimic acid, resveratrol etc. Methods applied hereunder for investigating the structural properties of GDH-synthesized RNA included purification of GDH isoenzymes, synthesis of RNA by the isoenzymes, reverse transcription of the RNA to cDNA, sequencing of the cDNA, computation of the G+C-contents, profiling the stability through PCR amplification compared with genetic code-based DNA; and biochemical characterization of the RNAs synthesized by individual hexameric isoenzymes of GDH. Single product bands resulted from the PCR amplification of the cDNAs of GDH-synthesized RNA, whereas several bands resulted from the amplification of genetic code-based DNA. The cDNAs have wide G+C-contents (35% to 59%), whereas genetic code-based DNA has narrower G+C-contents (50% to 60%). The GDH β6 homo-hexameric isoenzyme synthesized the A+U-rich RNAs, whereas the a6, and α6 homo-hexameric isoenzymes synthesized the G+C-rich RNAs. Therefore, the RNA synthesized by GDH is different from genetic code-based RNAs. In vitro chemical reactions revealed that GDH-synthesized RNA degraded total RNA to lower molecular weight products. Therefore, GDH-synthesized RNA is RNA enzyme. Dismantling of the structural constraints imposed on RNA by genetic code liberated RNA to become an enzyme with specificity to degrade unwanted transcripts. The RNA enzyme activity of GDH-synthesized RNA is ubiquitous in cells; it is readily induced by treatment of plants with mineral nutrients etc. and may simplify experimental approaches in plant How to cite this paper: Osuji, G.O. and Johnson, P.M. (2018) Structural Properties of the RNA Synthesized by Glutamate Dehydrogenase for the Degradation of Total RNA. Advances in Enzyme Research,

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Optimization of Cowpea Dry Grain Yield through the Stimulation of the RNA Synthetic Activity of NADH-Glutamate Dehydrogenase

Osuji¹,

Johnson²,

Madu³

2021

AJPS

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Several potentially practical biochemical processes in plant systems still remain hidden, especially the NADH-glutamate dehydrogenase (GDH) synthesis of nongenetic code-based RNA that optimizes crop nutritious yield by degrading superfluous genetic code-based RNA. In continued characterization of the biochemistry of cowpea grain yield, GDH was purified by electrophoresis from seeds of cowpea treated with solutions of stoichiometric mixes of mineral salts. The GDH was made to synthesize RNAs in the amination (α-KG/NADH/

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Molecular Regulation of the Metabolic Pathways of the Medicinal Plants: <i>Phyla dulcis</i>

Cited by 3 publications

References 24 publications

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Structural Properties of the RNA Synthesized by Glutamate Dehydrogenase for the Degradation of Total RNA

Optimization of Cowpea Dry Grain Yield through the Stimulation of the RNA Synthetic Activity of NADH-Glutamate Dehydrogenase

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