Molecular regulation of the salicylic acid hormone pathway in plants under changing environmental conditions

Rossi, C. R.; Marchetta, Eric J R; Kim, Jong Hum; Castroverde, Christian Danve M.

doi:10.1016/j.tibs.2023.05.004

Cited by 14 publications

(5 citation statements)

References 86 publications

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“…Salicylic acid (SA) is a signal molecule (plant hormone) involved in systemic acquired resistance (SAR) by triggering the plant immune system and activating defense and pathogenesis-related genes (Stroud et al, 2022; Rossi et al, 2023). Previously, researchers have used various formulations of Si and SA to improve abiotic stresses by inducing antioxidant enzymes (Khan et al, 2019; Yang et al, 2021; Shohani et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

Identification of cell-type-specific response to silicon treatment in soybean leaves through single nucleus RNA-sequencing

Devkar,

D’Agostino,

Kshetry

et al. 2024

Preprint

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In agriculture, mineral nutrients uptake and deposition profoundly influence plant development, stress resilience, and productivity. Despite its classification as a non-essential element, silicon (Si) is crucial in plant physiology, particularly in defense response and stress mitigation. While genetic and molecular mechanisms of Si uptake and transport are well-studied in monocots, particularly rice, its role in dicot species, such as soybean, remains unclear at the cellular and molecular levels. Traditional bulk transcriptomics methods lack the resolution to uncover cellular heterogeneity. Here, we present a study by utilizing single-nucleus RNA sequencing (snRNA-seq) to dissect cellular responses to Si accumulation in soybean leaves. Our analysis revealed distinct cellular populations, including a novel Si-induced cell cluster within vascular cells, suggesting a specific mechanism of Si distribution. Si treatment induced the expression of defense-related genes, particularly enriched in vascular cells, highlighting their specialized role in activating plant defense mechanisms. Moreover, Si modulated the expression of genes involved in RNA silencing, phytoalexin biosynthesis, and immune receptor signaling, suggesting a mechanism of transcriptional priming of genes involved in defense responses. We further investigated putative Si transporters, revealing differential expression patterns in response to Si treatment, suggesting presence of active and gradient-based transport mechanisms. Our findings shed light on the vital biotic stress regulatory networks governed by Si treatment in soybean leaves, paving potential strategies for enhancing stress tolerance and agronomic performance in crops.

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Section: Resultsmentioning

confidence: 99%

Identification of cell-type-specific response to silicon treatment in soybean leaves through single nucleus RNA-sequencing

Devkar,

D’Agostino,

Kshetry

et al. 2024

Preprint

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“…Similarly, JAs are also essential plant hormones that regulate plant development and environmental adaptation, and they can resist bacterial infection and increase plant tolerance to abiotic stresses, such as ozone, ultraviolet light, high temperature, and freezing damage [ 43 , 44 , 45 ]. SA, ABA, and JA act alone or interact with each other or with other hormones among signaling pathways, which are indispensable in the defense against plant pathogens [ 46 , 47 , 48 ]. In this study, JA, ABA, and SA were highly enriched during bulbil initiation, showing the same patterns of change.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Mou,

Zhang,

Qiu

et al. 2024

IJMS

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Pinellia ternata is a medicinal plant that has important pharmacological value, and the bulbils serve as the primary reproductive organ; however, the mechanisms underlying bulbil initiation remain unclear. Here, we characterized bulbil development via histological, transcriptomic, and targeted metabolomic analyses to unearth the intricate relationship between hormones, genes, and bulbil development. The results show that the bulbils initiate growth from the leaf axillary meristem (AM). In this stage, jasmonic acid (JA), abscisic acid (ABA), isopentenyl adenosine (IPA), and salicylic acid (SA) were highly enriched, while indole-3-acetic acid (IAA), zeatin, methyl jasmonate (MeJA), and 5-dexoxystrigol (5-DS) were notably decreased. Through OPLS-DA analysis, SA has emerged as the most crucial factor in initiating and positively regulating bulbil formation. Furthermore, a strong association between IPA and SA was observed during bulbil initiation. The transcriptional changes in IPT (Isopentenyltransferase), CRE1 (Cytokinin Response 1), A-ARR (Type-A Arabidopsis Response Regulator), B-ARR (Type-B Arabidopsis Response Regulator), AUX1 (Auxin Resistant 1), ARF (Auxin Response Factor), AUX/IAA (Auxin/Indole-3-acetic acid), GH3 (Gretchen Hagen 3), SAUR (Small Auxin Up RNA), GA2ox (Gibberellin 2-oxidase), GA20ox (Gibberellin 20-oxidase), AOS (Allene oxide synthase), AOC (Allene oxide cyclase), OPR (Oxophytodienoate Reductase), JMT (JA carboxy l Methyltransferase), COI1 (Coronatine Insensitive 1), JAZ (Jasmonate ZIM-domain), MYC2 (Myelocytomatosis 2), D27 (DWARF27), SMAX (Suppressor of MAX2), PAL (Phenylalanine Ammonia-Lyase), ICS (Isochorismate Synthase), NPR1 (Non-expressor of Pathogenesis-related Genes1), TGA (TGACG Sequence-specific Binding), PR-1 (Pathogenesis-related), MCSU (Molybdenium Cofactor Sulfurase), PP2C (Protein Phosphatase 2C), and SnRK (Sucrose Non-fermenting-related Protein Kinase 2) were highly correlated with hormone concentrations, indicating that bulbil initiation is coordinately controlled by multiple phytohormones. Notably, eight TFs (transcription factors) that regulate AM initiation have been identified as pivotal regulators of bulbil formation. Among these, WUS (WUSCHEL), CLV (CLAVATA), ATH1 (Arabidopsis Thaliana Homeobox Gene 1), and RAX (Regulator of Axillary meristems) have been observed to exhibit elevated expression levels. Conversely, LEAFY demonstrated contrasting expression patterns. The intricate expression profiles of these TFs are closely associated with the upregulated expression of KNOX(KNOTTED-like homeobox), suggesting a intricate regulatory network underlying the complex process of bulbil initiation. This study offers a profound understanding of the bulbil initiation process and could potentially aid in refining molecular breeding techniques specific to P. ternata.

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“…SA biosynthetic pathways mediated by both isochorismate synthase (ICS) and phenylalanine ammonia lyase (PAL) are regulated by abiotic derived from changes in the climate. The SA transcriptional coactivator NPR1 is positively regulated by chilling condition and suppressed by drought and high humidity(Rossi et al 2023 ). The changing of abiotic stress impacts the key component of SA pathway through its biosynthesis levels, signaling, metabolism and transport in plants.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The genetic orchestra of salicylic acid in plant resilience to climate change induced abiotic stress: critical review

Elsisi,

Elshiekh,

Sabry

et al. 2024

Stress Biology

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Climate change, driven by human activities and natural processes, has led to critical alterations in varying patterns during cropping seasons and is a vital threat to global food security. The climate change impose several abiotic stresses on crop production systems. These abiotic stresses include extreme temperatures, drought, and salinity, which expose agricultural fields to more vulnerable conditions and lead to substantial crop yield and quality losses. Plant hormones, especially salicylic acid (SA), has crucial roles for plant resiliency under unfavorable environments. This review explores the genetics and molecular mechanisms underlying SA's role in mitigating abiotic stress-induced damage in plants. It also explores the SA biosynthesis pathways, and highlights the regulation of their products under several abiotic stresses. Various roles and possible modes of action of SA in mitigating abiotic stresses are discussed, along with unraveling the genetic mechanisms and genes involved in responses under stress conditions. Additionally, this review investigates molecular pathways and mechanisms through which SA exerts its protective effects, such as redox signaling, cross-talks with other plant hormones, and mitogen-activated protein kinase pathways. Moreover, the review discusses potentials of using genetic engineering approaches, such as CRISPR technology, for deciphering the roles of SA in enhancing plant resilience to climate change related abiotic stresses. This comprehensive analysis bridges the gap between genetics of SA role in response to climate change related stressors. Overall goal is to highlight SA's significance in safeguarding plants and by offering insights of SA hormone for sustainable agriculture under challenging environmental conditions.

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Molecular regulation of the salicylic acid hormone pathway in plants under changing environmental conditions

Cited by 14 publications

References 86 publications

Identification of cell-type-specific response to silicon treatment in soybean leaves through single nucleus RNA-sequencing

Identification of cell-type-specific response to silicon treatment in soybean leaves through single nucleus RNA-sequencing

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

The genetic orchestra of salicylic acid in plant resilience to climate change induced abiotic stress: critical review

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