2011
DOI: 10.1593/neo.11138
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Molecular Requirements for Transformation of Fallopian Tube Epithelial Cells into Serous Carcinoma

Abstract: Although controversial, recent studies suggest that serous ovarian carcinomas may arise from fallopian tube fimbria rather than ovarian surface epithelium. We developed an in vitro model for serous carcinogenesis in which primary human fallopian tube epithelial cells (FTECs) were exposed to potentially oncogenic molecular alterations delivered by retroviral vectors. To more closely mirror in vivo conditions, transformation of FTECs was driven by the positive selection of growth-promoting alterations rather ant… Show more

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Cited by 66 publications
(63 citation statements)
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“…Culture conditions that more closely resemble the tumour microenvironment — including three-dimensional (3D) matrices and co-cultures of malignant cells with fibroblasts and mesothelial cells 40,41 — may also improve success in obtaining continuous, biologically relevant cell lines with stable biologic features. Immortalized FTSECs seem to be the most appropriate normal control for HGSOC 4244 , but further molecular and functional characterization is required of the handful of currently available FTSEC lines 42 . Consideration should be given to generating additional ovarian surface epithelial lines to better understand the role of these cells in HGSOC development.…”
Section: Improve Current Experimental Modelsmentioning
confidence: 99%
“…Culture conditions that more closely resemble the tumour microenvironment — including three-dimensional (3D) matrices and co-cultures of malignant cells with fibroblasts and mesothelial cells 40,41 — may also improve success in obtaining continuous, biologically relevant cell lines with stable biologic features. Immortalized FTSECs seem to be the most appropriate normal control for HGSOC 4244 , but further molecular and functional characterization is required of the handful of currently available FTSEC lines 42 . Consideration should be given to generating additional ovarian surface epithelial lines to better understand the role of these cells in HGSOC development.…”
Section: Improve Current Experimental Modelsmentioning
confidence: 99%
“…These observations suggest that pelvic serous carcinomas previously assigned to different sites of origin (ovary, FT, or peritoneum) share a common carcinogenic pathway not previously appreciated, which originates in the FT secretory epithelial cell. Experimental model systems, including novel genetically engineered animal models (3537) and cell-based assays (3840), have provided robust support for this new paradigm of ovarian cancer pathogenesis.…”
Section: Introductionmentioning
confidence: 99%
“…To further establish the correlation between Rad6 and OC we analyzed a normal, immortalized cell line derived from fallopian tube epithelial cells, a proposed OC precursor [13], and several OC cell lines, including 2 isogenic pairs of platinum-sensitive (A2780 and SKOV3) and platinum-resistant (A2780/CP70 and SKOV3/CP20) lines. As expected the OC cell lines had higher Rad6 expression than normal cells (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Fallopian tube epithelial cells (FTSEC or FTEC) were used as normal ovarian cells (generously provided by Dr. Amir Jazaeri) [13]. OV90 and SKOV3 cells were purchased from ATCC, isogenic A2780 (cisplatin-sensitive) and A2780/CP70 (cisplatin-resistant) cells were previously described [14].…”
Section: Methodsmentioning
confidence: 99%