Molecular Research on Muscle Protein and Myopathies

Karpicheva, Olga E.

doi:10.3390/ijms23137098

IJMS

2022

DOI: 10.3390/ijms23137098

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Molecular Research on Muscle Protein and Myopathies

Olga E. Karpicheva

Abstract: This Special Issue highlights new data on the molecular mechanisms of muscle functioning under normal conditions and cellular dysfunctions [...]

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2024

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Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis

Vantaggiato,

Landi,

Shaba

et al. 2024

Proteomes

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Muscle tissue is one of the most dynamic and plastic tissues of the mammalian body and covers different roles, such as force generation and metabolic control. Muscular proteomics provides an important opportunity to reveal the molecular mechanisms behind muscle pathophysiology. To ensure successful proteomic analysis, it is necessary to have an efficient and reproducible protein extraction method. This study aimed to evaluate the efficacy of two different extraction protocols of muscle samples for two-dimensional gel electrophoresis. In particular, mouse muscle proteins were extracted by an SDS-based buffer (Method A) and by a UREA/CHAPS/DTE/TRIS solution (Method B). The efficacies of the methods were assessed by performing an image analysis of the 2DE gels and by statistical and multivariate analyses. The 2DE gels in both preparations showed good resolution and good spot overlapping. Methods A and B produced 2DE gels with different means of total spots, higher for B. Image analysis showed different patterns of protein abundance between the protocols. The results showed that the two methods extract and solubilize proteins with different chemical–physical characteristics and different cellular localizations. These results attest the efficacy and reproducibility of both protein extraction methods, which can be parallelly applied for comprehensive proteomic profiling of muscle tissue.

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Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis

Vantaggiato,

Landi,

Shaba

et al. 2024

Proteomes

View full text Add to dashboard Cite

show abstract

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Molecular Research on Muscle Protein and Myopathies

Abstract: This Special Issue highlights new data on the molecular mechanisms of muscle functioning under normal conditions and cellular dysfunctions [...]

Cited by 1 publication

References 9 publications

Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis

Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis

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