Molecular scaffolds for three-dimensional cell and tissue cultures

Kurzepa, Katarzyna; Różycki, Krzysztof; Bochynska, Marta; Konop, Marek; Urbańczyk‐Lipkowska, Zofia; Lipkowski, Andrzej W.

doi:10.14314/polimery.2013.663

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…The latter are responsible for the formation of a rigid hair shaft through extensive disulfide bond cross-linking of keratin filaments that makes hair extremely resistant either to chemical or biological degrading agents. [11][12][13][14] Keratin derivatives which can be soluble or insoluble are utilized as wound dressings, since keratins are dynamically up-regulated and needed in skin wound healing. Especially keratins type 6,16, and 17 are involved in skin recovery upon injury and in keratin 17 knock-out mice, poor healing is observed.…”

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confidence: 99%

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The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Konop

Sulejczak

Czuwara

et al. 2017

Wound Repair Regeneration

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Keratin is an interesting protein needed for wound healing and tissue recovery. We have recently proposed a new, simple and inexpensive method to obtain fur and hair keratin-derived biomaterials suitable for medical application. The aim of the study was to evaluate the role of the fur keratin-derived protein (FKDP) dressing in the allogenic full-thickness surgical skin wound model. The data obtained using scanning electron microscopy showed that employed processed biomaterial had higher surface porosity compared with control raw material. From the MTS test, it was found keratin biomaterial is not only toxic to the NIH/3T3 cell line (p < 0.05), but also enhances cell proliferation compared with the control. In vivo studies have shown keratin dressings are tissue biocompatible, accelerate wound closure and epithelialization to the statistically significant differences on day 5 (p < 0.05) in comparison to control wounds. Histological examination revealed, that in FKDP-treated wounds the inflammatory response contained predominantly macrophages whilst their morphological untreated variants showed mixed cell infiltrates rich in neutrophils. Predominant macrophages based response creates more favorable environment for healing. In FKDP-dressed wounds the number of microhemorrhages was also significantly decreased (p < 0.05) as compared with undressed wounds. Applied keratin dressing favors reconstruction of a more regular skin structure and assures better cosmetic effect in terms of scar formation and appearance. In conclusion, fur keratin-derived protein dressings significantly accelerated wound healing in the mouse model. Further studies are needed to determine the molecular mechanisms involved in the multilayer wound healing process and to assess the possible use of these dressings for medical purposes.

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confidence: 99%

“…Keratins and keratin‐associated proteins are the major structural components of hair fibers. The latter are responsible for the formation of a rigid hair shaft through extensive disulfide bond cross‐linking of keratin filaments that makes hair extremely resistant either to chemical or biological degrading agents …”

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confidence: 99%

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Konop

Sulejczak

Czuwara

et al. 2017

Wound Repair Regeneration

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“…One such example of a synthetic polymer is poly(lactic-co-glycolic) acid (PLGA) 38. The polymer degrades via hydrolysis of the ester linkages into lactic and glycolic acid which can be further degraded in the body as they are common components for metabolism in cells during the Cori cycle 39. By varying the ratio of the lactic and glycolic acid subunits in the PLGA polymer, the degradation of the polymer can be controlled 40.…”

Section: Bm Te Approachesmentioning

confidence: 99%

Tissue engineering of retina and Bruch’s membrane: a review of cells, materials and processes

et al. 2018

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The biological, structural and functional configuration of Bruch's membrane (BM) is significantly relevant to age-related macular degeneration (AMD) and other chorioretinal diseases, and AMD is one of the leading causes of blindness in the elderly worldwide. The configuration may worsen along with the ageing of retinal pigment epithelium and BM that finally leads to AMD. Thus, the scaffold-based tissue-engineered retina provides an innovative alternative for retinal tissue repair. The cell and material requirements for retinal repair are discussed including cell sheet engineering, decellularised membrane and tissue-engineered membranes. Further, the challenges and potential in realising a whole tissue model construct for retinal regeneration are highlighted herein. This review article provides a framework for future development of tissue-engineered retina as a preclinical model and possible treatments for AMD.

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“…Keratin materials have been extensively used in tissue engineering and regenerative medicine owing to their biological function, structural support, excellent biocompatibility, and favorable biodegradability characteristics. Accordingly, these properties allow scientists to create a new type of wound dressing that can enhance the healing process especially in chronic Pharmaceutics 2021, 13, 2029 2 of 20 non-healing wounds [6][7][8][9][10]. They can be cast as sponges, films, and hydrogels for various biomedical applications [11].…”

Section: Introductionmentioning

confidence: 99%

Keratin Biomaterials in Skin Wound Healing, an Old Player in Modern Medicine: A Mini Review

2021

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Impaired wound healing is a major medical problem. To solve it, researchers around the world have turned their attention to the use of tissue-engineered products to aid in skin regeneration in case of acute and chronic wounds. One of the primary goals of tissue engineering and regenerative medicine is to develop a matrix or scaffold system that mimics the structure and function of native tissue. Keratin biomaterials derived from wool, hair, and bristle have been the subjects of active research in the context of tissue regeneration for over a decade. Keratin derivatives, which can be either soluble or insoluble, are utilized as wound dressings since keratins are dynamically up-regulated and needed in skin wound healing. Tissue biocompatibility, biodegradability, mechanical durability, and natural abundance are only a few of the keratin biomaterials’ properties, making them excellent wound dressing materials to treat acute and chronic wounds. Several experimental and pre-clinical studies described the beneficial effects of the keratin-based wound dressing in faster wound healing. This review focuses exclusively on the biomedical application of a different type of keratin biomaterials as a wound dressing in pre-clinical and clinical conditions.

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Molecular scaffolds for three-dimensional cell and tissue cultures

Cited by 5 publications

References 8 publications

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Tissue engineering of retina and Bruch’s membrane: a review of cells, materials and processes

Keratin Biomaterials in Skin Wound Healing, an Old Player in Modern Medicine: A Mini Review

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