Molecular screening for Anaplasmataceae in ticks and tsetse flies from Ethiopia

Hornok, Sándor; Abichu, Getachew; Takács, Nóra; Gyuranecz, Miklós; Farkas, Róbert; Mera, Isabel G. Fernández de; Fuente, José de la

doi:10.1556/004.2016.007

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…Evidence of the presence of A. phagocytophilum in Africa has been reported from Morocco, Algeria and Tunisia [23, 54, 67], where Ixodes ricinus has been identified in some foci in the northern regions of these countries [18]. This infection has also been reported from wildlife in Zimbabwe and South Africa [68, 69], and from some African ticks such as Ambyomma flavomaculatum collected from two different specimens of lizard ( Varanus exanthematicus ) imported to Poland from Ghana [70], from A. cohaerens , A. lepidum and A. variegatum in Ethiopia [71, 72] and from Hy. marginatum in Tunisia [53].…”

Section: Discussionmentioning

confidence: 92%

Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

et al. 2019

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BackgroundOur study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal).MethodsA total of 204 ticks and 433 blood samples were collected from ruminants, horses, donkeys and dogs. Ticks were identified morphologically and by molecular characterization targeting the 12S rRNA gene. Molecular characterization of species of Anaplasmataceae infecting Senegalese ticks and animals was conducted using the 23S rRNA, 16S rRNA, rpoB and groEL genes.ResultsTicks were identified as Rhipicephalus evertsi evertsi (84.3%), Hyalomma rufipes (8.3%), Hyalomma impeltatum (4.9%), R. bursa (1.5%) and R. muhsamae (0.9%). The overall prevalence of Anaplasmataceae infection in ticks was 0.9%, whereas 41.1% of the sampled animals were found infected by one of the species belonging to this family. We identified the pathogen Anaplasma ovis in 55.9% of sheep, A. marginale and A. centrale in 19.4% and 8.1%, respectively, of cattle, as well as a putative new species of Anaplasmataceae. Two Anaplasma species commonly infecting ruminants were identified. Anaplasma cf. platys, closely related to A. platys was identified in 19.8% of sheep, 27.7% of goats and 22.6% of cattle, whereas a putative new species, named here provisionally “Candidatus Anaplasma africae”, was identified in 3.7% of sheep, 10.3% of goats and 8.1% of cattle. Ehrlichia canis and Anaplasma platys were identified only from dogs sampled in the Keur Momar Sarr area. Ehrlichia canis was identified in 18.8% of dogs and two R. e. evertsi ticks removed from the same sheep. Anaplasma platys was identified in 15.6% of dogs. Neither of the dogs sampled from Casamance region nor the horses and donkeys sampled from Keur Momar Sarr area were found infected by an Anaplasmataceae species.ConclusionsThis study presents a summary of Anaplasmataceae species that infect animals and ticks in three areas from the northern, central and southern regions of Senegal. To our knowledge, our findings demonstrate for the first time the presence of multiple Anaplasmataceae species that infect ticks and domestic animals in Senegal. We recorded two potentially new species commonly infecting ruminants named here provisionally as Anaplasma cf. platys and “Candidatus Anaplasma africae”. However, E. canis was the only species identified and amplified from ticks. None of the other Anaplasmataceae species identified in animals were identified in the tick species collected from animals.

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Section: Discussionmentioning

confidence: 92%

Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

et al. 2019

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“…Meanwhile, Am. cohaerens , which circulates in many areas including in western and central regions, was found to transmit A. marginale [ 72 ] and A. phagocytophilum [ 18 ], which is a zoonotic pathogen causing human granulocytic anaplasmosis. In Europe, however, this zoonotic infection is transmitted by Ixodes ricinus [ 76 ], confirming that TBPs can use different tick species depending on their distribution.…”

Section: Discussionmentioning

confidence: 99%

“…Indigenous breeds of livestock are resilient to ticks and TBPs, but genetically improved breeds, which contribute to sustainable food security, are highly susceptible [ 14 , 15 ]. Various TBPs have been identified from animals [ 12 , 15 , 16 ] and from ticks themselves [ 17 , 18 ]. However, the problem does not seem uniform across all agroecological zones, as several ticks known to be vectors for highly pathogenic agents for both improved and local breeds of animals have been found to select suitable agroecology for their survival and multiplication.…”

Section: Introductionmentioning

confidence: 99%

Geographical distribution of ixodid ticks and tick-borne pathogens of domestic animals in Ethiopia: a systematic review

Kaba

2022

Parasites Vectors

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Background In Ethiopia, ixodid ticks and associated tick-borne pathogens (TBPs) are of great importance from both a veterinary and public health point of view. This review aimed at compiling available published data on the distribution of ixodid tick species and TBPs in the country. Methods A standard review approach was employed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline. Published peer-reviewed articles and theses/dissertations reporting on ixodid ticks and TBPs in Ethiopia were searched using different keywords in many electronic databases including PubMed, Scopus, Web of Science, Google Scholar, African Journals OnLine, and institutional repositories. Articles were screened based on inclusion and exclusion criteria using the PRISMA flowchart. Data were retrieved from eligible articles and recorded in a preformed data record sheet. Descriptive statistics were employed to present data using graphs. QGIS (Quantum GIS) software version 3.4.5 was used to show the distribution of ixodid tick species and TBPs. Results Overall, 35 articles that met the inclusion criteria were included in this review. Of these, 24 articles report only on ixodid ticks of domestic animals, six articles report only on TBPs in livestock or ticks, and five articles report on both ticks and TBPs in either animals or ticks. Of these studies, 54% were in the Oromia region, while only 3% of studies were in the Benishangul-Gumuz region. The Gambela region lacked studies on ticks and TBPs. At least 19 ixodid tick species have been recorded from different domestic animals including cattle, small ruminants, donkeys, horses, camels, dogs, and cats. Morphological characterization appears to be the sole method of tick species identification in the country. The distribution and abundance of specific tick species depend on geographical locations and agroecological factors. Sixteen molecularly confirmed TBPs have been identified in animal and tick tissue using molecular methods from only four administrative regions, despite the wide distribution of ticks. Among TBPs, five Anaplasma, two Ehrlichia, two Rickettsia, five Theileria, two Babesia, and one Coxiella species are the major pathogens in both livestock and humans. Conclusions Many ixodid ticks circulate in a wide geographical zone of Ethiopia. However, the limited reports on TBPs at the country level in general, and the absence of either tick or TBP reports around the border region with neighboring countries in particular, highlights the need for further study. Graphical Abstract

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“…The following supporting information can be downloaded at: , Table S1: PRISMA checklist and additional checklist based on Migliavaca et al, 2020, guidelines; Table S2: List of papers excluded during full-text examination and relevant exclusion criteria; Table S3: Details of qualitative analysis; Table S4: Details of critical appraisal. References [ 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 ,…”

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confidence: 99%

Distribution and Prevalence of Anaplasmataceae, Rickettsiaceae and Coxiellaceae in African Ticks: A Systematic Review and Meta-Analysis

et al. 2023

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In Africa, ticks continue to be a major hindrance to the improvement of the livestock industry due to tick-borne pathogens that include Anaplasma, Ehrlichia, Rickettsia and Coxiella species. A systemic review and meta-analysis were conducted here and highlighted the distribution and prevalence of these tick-borne pathogens in African ticks. Relevant publications were searched in five electronic databases and selected using inclusion/exclusion criteria, resulting in 138 and 78 papers included in the qualitative and quantitative analysis, respectively. Most of the studies focused on Rickettsia africae (38 studies), followed by Ehrlichia ruminantium (27 studies), Coxiella burnetii (20 studies) and Anaplasma marginale (17 studies). A meta-analysis of proportions was performed using the random-effects model. The highest prevalence was obtained for Rickettsia spp. (18.39%; 95% CI: 14.23–22.85%), R. africae (13.47%; 95% CI: 2.76–28.69%), R. conorii (11.28%; 95% CI: 1.77–25.89%), A. marginale (12.75%; 95% CI: 4.06–24.35%), E. ruminantium (6.37%; 95% CI: 3.97–9.16%) and E. canis (4.3%; 95% CI: 0.04–12.66%). The prevalence of C. burnetii was low (0%; 95% CI: 0–0.25%), with higher prevalence for Coxiella spp. (27.02%; 95% CI: 10.83–46.03%) and Coxiella-like endosymbionts (70.47%; 95% CI: 27–99.82%). The effect of the tick genera, tick species, country and other variables were identified and highlighted the epidemiology of Rhipicephalus ticks in the heartwater; affinity of each Rickettsia species for different tick genera; dominant distribution of A. marginale, R. africae and Coxiella-like endosymbionts in ticks and a low distribution of C. burnetii in African hard ticks.

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Molecular screening for Anaplasmataceae in ticks and tsetse flies from Ethiopia

Cited by 10 publications

References 13 publications

Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

Geographical distribution of ixodid ticks and tick-borne pathogens of domestic animals in Ethiopia: a systematic review

Distribution and Prevalence of Anaplasmataceae, Rickettsiaceae and Coxiellaceae in African Ticks: A Systematic Review and Meta-Analysis

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