Molecular Screening for High-Risk Human Papillomaviruses in Patients with Periodontitis

Shishkova, Kalina; Gergova, Raina; Tasheva‐Terzieva, Elena; Shishkov, Stoyan; Sirakov, Ivo

doi:10.3390/v15030809

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…59 The supporting view believes that interaction between virus and bacteria can account for the phenomenon, as the overlapping trends between HPV load and the number of periodontitis-causing bacteria have been observed. 60 In addition, the composition of microbial populations also changed during periodontitis with HPV infection, 61 which could disturb the balance in the oral microbiome and induce disease. The differences in the measurements and definition of periodontitis or HPV infection can account for the discrepancy between studies.…”

Section: F I G U R Ementioning

confidence: 99%

Association between virus infection and periodontitis: Evidence from the National Health and Nutrition Examination Survey 2009−2014

Fang,

Liu,

Mao

et al. 2024

Journal of Medical Virology

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Periodontitis is a cumulative inflammatory disease associated with multiple health conditions and various systemic diseases. As a common disease, virus infection along with its consequences has become a serious health burden. The study aims to evaluate the relationship between common viruses including hepatitis virus, human immunodeficiency virus (HIV), herpes simplex virus (HSV), human papillomavirus (HPV), and periodontitis. The data from the US National Health and Nutrition Examination Survey (NHANES) 2009−2014 was adopted and screened through, including 10 714 participants. Generalized linear regression was conducted to verify the relationships between the virus infections and periodontitis. Moreover, we also performed analyses in age and gender subgroups. The results suggested that the infection of HCV, HSV‐1, and HSV‐2 was significantly associated with the prevalence of periodontitis (odds ratio [OR] 1.46, 95% confidence interval [CI] 1.26−1.70; OR 1.09, 95% CI 1.05−1.13; OR 1.06, 95% CI 1.01 ‐ 1.11, respectively) and risk of developing moderate or severe periodontitis (OR 1.51, 95% CI 1.29−1.77; OR 1.08, 95% CI 1.04−1.12; OR 1.05, 95% CI 1.01−1.10, respectively) after adjusting all relevant co‐factors. Subgroup analyses revealed a steady association between periodontitis and hepatitis C virus (HCV) or HSV‐1 infection, while the relationship between HSV‐2 and HPV infection can also be found in some subgroups. The presence of HCV and HSV infection was found to be significantly associated with the prevalence of periodontitis, including moderate or severe cases. Moreover, the association of periodontitis and HPV infection can also be observed in people < 35 years.

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Section: F I G U R Ementioning

confidence: 99%

Association between virus infection and periodontitis: Evidence from the National Health and Nutrition Examination Survey 2009−2014

Fang,

Liu,

Mao

et al. 2024

Journal of Medical Virology

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Field-Applicable Loop-Mediated Isothermal Amplification for the Detection of Seven Common Human Papillomavirus Subtypes

Li,

Tan,

et al. 2024

TropicalMed

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Persistent HPV infection is a major risk factor for the subsequent development of cervical cancer. LAMP is simple and suitable for field detection in the resource-limited settings. In this study, hydroxy naphthol blue (HNB)-based visual LAMP and evagreen-based fluorescent LAMP coupled with a microfluidic chip (LAMP-chip) were established for the field detection of seven subtypes of HPV. The analytical sensitivity was 19–233 copies/reaction. The overall clinical sensitivity was 97.35% for visual LAMP and 98.23% for LAMP-chip. Both LAMP assays exhibited 100% specificity and were completed in less than 50 min. Additionally, both assays did not require complicated nucleic acid extraction and purification steps. A complete quality control monitoring system (including internal control, positive quality control and negative control) in the LAMP assays further ensured the credibility of the results. Our findings demonstrated that the proposed LAMP assays have the potential to be applied in the testing of common HPV DNA in field investigations (visual LAMP) or within communities and primary health centers (LAMP-chip).

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Molecular Screening for High-Risk Human Papillomaviruses in Patients with Periodontitis

Cited by 2 publications

References 29 publications

Association between virus infection and periodontitis: Evidence from the National Health and Nutrition Examination Survey 2009−2014

Association between virus infection and periodontitis: Evidence from the National Health and Nutrition Examination Survey 2009−2014

Field-Applicable Loop-Mediated Isothermal Amplification for the Detection of Seven Common Human Papillomavirus Subtypes

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