Molecular screening of RND-type efflux pumps genes among Pseudomonas aeruginosa isolated from burn patients in Najaf City, Iraq

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is a main clinical opportunistic pathogen that causes of morbidity and mortality among burn patients. therefore this study aimed to detection of RND-type efflux pump genes among P. aeruginosa isolates in Najaf City, using PCR technique. The study involved 68 specimen's obtained from burn patients visited burns unit center as well as some chief clinical laboratories in Najaf City period December 2021 to March 2022. This study showed among 68 burn patients 47(69.11%) and 12 (17.64%) of positive bacterial growth returned to gram-negative and gram-positive bacteria respectively. In contrast, 9(13.23%) of specimens were no growth. In the same respect, the results according to total positive bacterial growth have shown that the rate of P. aeruginosa isolates was 32(54.23%) At same as following 12(20.33%) in men compared with 20(33.89%) in women. The molecular results of efflux pump genes in this study revealed that 31 (96.87%%) of P. aeruginosa isolates were contained MexA and MexD genes, While 30(93.75%) of isolates were positive for MexB gene. Moreover, 25(78.12%) of isolates were harbored MexC and MexY genes, as well as 23(71.87%) of isolates were harbored MexX gene.