Molecular Selection, Modification and Development of Therapeutic Oligonucleotide Aptamers

Yu, Yuanyuan; Liang, Chao; Lv, Quanxia; Li, Defang; Xu, Xuegong; Liu, Bao-Qin; Lu, Aiping; Zhang, Ge

doi:10.3390/ijms17030358

Cited by 51 publications

(39 citation statements)

References 83 publications

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“…Immobilization of aptamers on the surface of scaffolds and implants represents a valid approach with numerous advantages such as low cost, low immunogenicity, and high affinity to the target cell when compared to other classes of ligands including antibodies and peptides [73, 74]. To this date, aptamers have been primarily used for identifying and capturing cancer cells [75-77], but they can also be applied for targeted isolation and recruitment of stem cells [78, 79].…”

Section: Strategies To Achieve Stem and Progenitor Cell Recruitmentmentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

133

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A leading strategy in tissue engineering is the design of biomimetic scaffolds that stimulate the body’s repair mechanisms through the recruitment of endogenous stem cells to sites of injury. Approaches that employ the use of chemoattractant gradients to guide tissue regeneration without external cell sources are favored over traditional cell-based therapies that have limited potential for clinical translation. Following this concept, bioactive scaffolds can be engineered to provide a temporally and spatially controlled release of biological cues, with the possibility to mimic the complex signaling patterns of endogenous tissue regeneration. Another effective way to regulate stem cell activity is to leverage the inherent chemotactic properties of extracellular matrix (ECM)-based materials to build versatile cell-instructive platforms. This review introduces the concept of endogenous stem cell recruitment, and provides a comprehensive overview of the strategies available to achieve effective cardiovascular and bone tissue regeneration.

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Section: Strategies To Achieve Stem and Progenitor Cell Recruitmentmentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

133

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“…In the last two decades, aptamers have proved to be very attractive as therapeutic agents mainly for their enhanced cellular uptake, nonimmunogenicity and lower production costs [75]. Several aptamers against different human pathologies, such as neurodegenerative diseases [76], age-related macular degeneration [77], inflammation [78], thrombosis [79], diabetes [63] etc., have been discovered and evaluated.…”

Section: G4-forming Aptamers In Therapeutic Applicationsmentioning

confidence: 99%

“…AS1411 is a G4-forming 26-mer DNA aptamer, highly stable and resistant to nuclease degradation, targeting nucleolin. Nucleolin is a ubiquitous and multifunctional protein playing essential roles in cell survival, growth and proliferation [78]. AS1411 binds to the external domain of nucleolin, which is overexpressed on the surface of cancer cells.…”

Section: G4-based Aptamers As Drugsmentioning

confidence: 99%

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G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Platella

Riccardi

Montesarchio

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

144

116

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Nucleic acid aptamers are single-stranded DNA or RNA molecules identified to recognize with high affinity specific targets including proteins, small molecules, ions, whole cells and even entire organisms, such as viruses or bacteria. They can be identified from combinatorial libraries of DNA or RNA oligonucleotides by SELEX technology, an in vitro iterative selection procedure consisting of binding (capture), partitioning and amplification steps. Remarkably, many of the aptamers selected against biologically relevant protein targets are G-rich sequences that can fold into stable G-quadruplex (G4) structures. Aiming at disseminating novel inspiring ideas within the scientific community in the field of G4-structures, the emphasis of this review is placed on: 1) recent advancements in SELEX technology for the efficient and rapid identification of new candidate aptamers (introduction of microfluidic systems and next generation sequencing); 2) recurrence of G4 structures in aptamers selected by SELEX against biologically relevant protein targets; 3) discovery of several G4-forming motifs in important regulatory regions of the human or viral genome bound by endogenous proteins, which per se can result into potential aptamers; 4) an updated overview of G4-based aptamers with therapeutic potential and 5) a discussion on the most attractive G4-based aptamers for diagnostic applications. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio.

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“…So far, several variants of SELEX protocol have been successfully applied to the isolation of a wide range of aptamers against therapeutically relevant targets [1,2]. In most cases, aptamers provide their therapeutic effect by inhibiting their proper target and thus attenuating the corresponding downstream molecular pathway.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Mediated Targeted Delivery of Therapeutics: An Update

2016

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The selective delivery of drugs in a cell- or tissue-specific manner represents the main challenge for medical research; in order to reduce the occurrence of unwanted off-target effects. In this regard, nucleic acid aptamers have emerged as an attractive class of carrier molecules due to their ability to bind with high affinity to specific ligands; their high chemical flexibility; as well as tissue penetration capability. To date, different aptamer-drug systems and aptamer–nanoparticles systems, in which nanoparticles function together with aptamers for the targeted delivery, have been successfully developed for a wide range of therapeutics, including toxins; peptides; chemotherapeutics and oligonucleotides. Therefore, aptamer-mediated drug delivery represents a powerful tool for the safe and effective treatment of different human pathologies, including cancer; neurological diseases; immunological diseases and so on. In this review, we will summarize recent progress in the field of aptamer-mediated drug delivery and we will discuss the advantages, the achieved objectives and the challenges to be still addressed in the near future, in order to improve the effectiveness of therapies.

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Molecular Selection, Modification and Development of Therapeutic Oligonucleotide Aptamers

Cited by 51 publications

References 83 publications

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Aptamer-Mediated Targeted Delivery of Therapeutics: An Update

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