Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny

Li, Xiaochuang; Li, Shouchun; Kong, Renqiu; Li, Renhui

doi:10.1016/j.hal.2016.06.003

Cited by 11 publications

(4 citation statements)

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“…In Cylindrospermopsis , heterocytes are always terminal at one or both ends of trichomes, and the absence of this specialized cell is not unusual ( Komárek and Anagnostidis, 1989 ). Several attempts were made to solve the taxonomic relationship between Cylindrospermopsis and Raphidiopsis genera using molecular and phylogenetic approaches ( Gugger et al, 2005 ; Stucken et al, 2009 ; Alster et al, 2010 ; Wu et al, 2011 ; Li et al, 2016 ). Recently, a proposal to unify Cylindrospermopsis and Raphidiopsis genera under the single genus Rhaphidiopsis was published ( Aguillera et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Genomic and Genotypic Characterization of Cylindrospermopsis raciborskii: Toward an Intraspecific Phylogenetic Evaluation by Comparative Genomics

et al. 2018

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Cylindrospermopsis raciborskii is a freshwater cyanobacterial species with increasing bloom reports worldwide that are likely due to factors related to climate change. In addition to the deleterious effects of blooms on aquatic ecosystems, the majority of ecotypes can synthesize toxic secondary metabolites causing public health issues. To overcome the harmful effects of C. raciborskii blooms, it is important to advance knowledge of diversity, genetic variation, and evolutionary processes within populations. An efficient approach to exploring this diversity and understanding the evolution of C. raciborskii is to use comparative genomics. Here, we report two new draft genomes of C. raciborskii (strains CENA302 and CENA303) from Brazilian isolates of different origins and explore their molecular diversity, phylogeny, and evolutionary diversification by comparing their genomes with sequences from other strains available in public databases. The results obtained by comparing seven C. raciborskii and the Raphidiopsis brookii D9 genomes revealed a set of conserved core genes and a variable set of accessory genes, such as those involved in the biosynthesis of natural products, heterocyte glycolipid formation, and nitrogen fixation. Gene cluster arrangements related to the biosynthesis of the antifungal cyclic glycosylated lipopeptide hassallidin were identified in four C. raciborskii genomes, including the non-nitrogen fixing strain CENA303. Shifts in gene clusters involved in toxin production according to geographic origins were observed, as well as a lack of nitrogen fixation (nif) and heterocyte glycolipid (hgl) gene clusters in some strains. Single gene phylogeny (16S rRNA sequences) was congruent with phylogeny based on 31 concatenated housekeeping protein sequences, and both analyses have shown, with high support values, that the species C. raciborskii is monophyletic. This comparative genomics study allowed a species-wide view of the biological diversity of C. raciborskii and in some cases linked genome differences to phenotype.

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Section: Introductionmentioning

confidence: 99%

Genomic and Genotypic Characterization of Cylindrospermopsis raciborskii: Toward an Intraspecific Phylogenetic Evaluation by Comparative Genomics

et al. 2018

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“…Molecular phylogeny based on multiple gene loci sequences (i.e.,psbA,16S rDNA,rbcL,rbcS,rpoC1, and secondary structures of the 16S-23S ITS region failed to distinguish among species in the genus Raphidiopsis (Wu et al 2011, Aguilera et al 2018. Recently, only the results of the ITS-L phylogeny have indicated a separation of Raphidiopsis species (Li et al 2016). However, PCR amplification of ITS regions between the 16S rDNA and 23S rDNA of the cyanobacteria proved to be somewhat complicated by revealing two true ITS regions of different sizes.…”

Section: Discussionmentioning

confidence: 99%

“…The morphological difference between these species is the existence or not of heterocyte cells (Moustaka-Gouni et al 2009): R. raciborskii with straight and coiled trichomes develop heterocyte cells, while R. mediterranea, characterized by the straight trichomes, and R. curvata, distinguished by the coiled trichomes, do not develop heterocyte cells. Several attempts have been made to elucidate the taxonomic relationships between these species in the genus Raphidiopsis using molecular techniques; however, their phylogenetic relationships suggest a monophyletic group (Wu et al 2011, Komárek 2013, Li et al 2016, Aguilera et al 2018. In terms of toxicity, toxins produced by R. raciborskii are known to vary among strains: some Australian strains pro-duce CYN, while some Brazilian strains produce saxitoxin (STX) (Hoff-Risseti et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterizations of Thai <i>Raphidiopsis raciborskii</i> (Nostocales, Cyanobacteria) based on 16S rDNA, <i>rbcLX</i>, and cylindrospermopsin synthetase genes

Tawong

Pongcharoen

Nishimura

et al. 2019

Plankton Benthos Res

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Raphidiopsis (previously Cylindrospermopsis) raciborskii is considered as a cylindrospermopsin (CYN) producer in Thailand. There are no reports including molecular information on the genes associated with CYN production of cyanobacteria from Thailand. In the present study, 27 new R. raciborskii strains from Thailand were isolated. Their morphological features and phylogenetic positions based on the partial sequences of 16S rDNA and rbcLX were analyzed for identification. Morphological characteristics of all the strains tested, and the natural samples, were found to be similar to the original description of R. raciborskii. The rbcLX phylogenetic trees revealed that the clade of R. raciborskii is independent from those of R. mediterranea and R. curvata, whereas the 16S rDNA phylogenetic trees failed to discriminate these species. Thus, all the strains tested in the present study were identified as R. raciborskii. Furthermore, the LC/MS/MS analysis confirmed that some of the strains tested produced CYN and 7-deoxy-CYN. The presence of genes associated with CYN biosynthesis (cyrA, cyrB, cyrC, and cyrJ) in Thai Raphidiopsis strains was investigated by PCR and phylogenetic analyses. Results of PCR showed that the PCR amplicons of cyrB and cyrJ were obtained from all toxic strains, while the amplicons of cyrA and cyrC were not obtained in some toxic Thai strains. Our findings supported the observation that cyrB and cyrJ, especially the latter, may be useful for the detection of CYNproducing cyanobacteria in water resources for water supplies and fisheries.

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“…In contrast to the 16S rRNA gene, the RNA polymerase C1 (rpoC1) gene and ITS-L sequence, which is the larger fragment of the inter-transcribed sequence of rRNA, are useful molecular markers for discriminating different R. raciborskii strains [58,67]. The strains were classified into nontoxic, CYN-producing, and PSP-producing clusters based on the phylogenetic analysis of rpoC1 and ITS-L [58].…”

Section: Phenotypic and Genetic Diversitymentioning

confidence: 99%

Recent Advances in the Ecology of Bloom-Forming Raphidiopsis (Cylindrospermopsis) raciborskii: Expansion in China, Intraspecific Heterogeneity and Critical Factors for Invasion

Liu

et al. 2023

IJERPH

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Water blooms caused by the invasive cyanobacterium Raphidiopsis raciborskii occur in many reservoirs in the tropical and subtropical regions of China. In recent decades, this species has spread rapidly to temperate regions. Phenotypic plasticity and climate warming are thought to promote the worldwide dispersion of R. raciborskii. However, investigations into the genetic and phenotypic diversities of this species have revealed significant intraspecific heterogeneity. In particular, competition between R. raciborskii and Microcystis aeruginosa was highly strain dependent. Although the concept of an ecotype was proposed to explain the heterogeneity of R. raciborskii strains with different geographic origins, microevolution is more reasonable for understanding the coexistence of different phenotypes and genotypes in the same environment. It has been suggested that intraspecific heterogeneity derived from microevolution is a strong driving force for the expansion of R. raciborskii. Additionally, temperature, nutrient fluctuations, and grazer disturbance are critical environmental factors that affect the population establishment of R. raciborskii in new environments. The present review provides new insights into the ecological mechanisms underlying the invasion of R. raciborskii in Chinese freshwater ecosystems.

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Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny

Cited by 11 publications

References 34 publications

Genomic and Genotypic Characterization of Cylindrospermopsis raciborskii: Toward an Intraspecific Phylogenetic Evaluation by Comparative Genomics

Genomic and Genotypic Characterization of Cylindrospermopsis raciborskii: Toward an Intraspecific Phylogenetic Evaluation by Comparative Genomics

Molecular characterizations of Thai <i>Raphidiopsis raciborskii</i> (Nostocales, Cyanobacteria) based on 16S rDNA, <i>rbcLX</i>, and cylindrospermopsin synthetase genes

Recent Advances in the Ecology of Bloom-Forming Raphidiopsis (Cylindrospermopsis) raciborskii: Expansion in China, Intraspecific Heterogeneity and Critical Factors for Invasion

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