Molecular serotyping and antimicrobial resistance profiles of<i>Actinobacillus pleuropneumoniae</i>isolated from pigs in South Korea

Kim, Boram; Hur, Jin; Lee, Jiyeong; Choi, Yoonyoung; Lee, John Hwa

doi:10.1080/01652176.2016.1155241

Cited by 27 publications

(20 citation statements)

References 27 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The poor specificity of the detection methods for A. pleuropneumoniae and the rapid progression of the infection frequently prompt widespread application of antibiotic treatment on the mere suspicion of an infection [ 14 ]. This contributes to the overuse of antibiotics, which promotes selection of resistant bacteria and may indirectly lead to the spread of antibiotic resistance genes through the food chain [ 15 , 16 ]. Accordingly, an effective vaccine against A. pleuropneumoniae , which protects across the 16 serovars is urgently needed.…”

Section: Introductionmentioning

confidence: 99%

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Antenucci

Fougeroux

Deeney

et al. 2018

Vet Res

View full text Add to dashboard Cite

Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A. pleuropneumoniae, as the majority of Gram-negative bacteria, excrete vesicles from its outer membrane (OM), accordingly defined as outer membrane vesicles (OMVs). Thanks to their antigenic similarity to the OM, OMVs have emerged as a promising tool in vaccinology. In this study we describe the in vivo testing of several vaccine prototypes for the prevention of infection by all known A. pleuropneumoniae serotypes. Previously identified vaccine candidates, the recombinant proteins ApfA and VacJ, administered individually or in various combinations with the OMVs, were employed as vaccination strategies. Our data show that the addition of the OMVs in the vaccine formulations significantly increased the specific IgG titer against both ApfA and VacJ in the immunized animals, confirming the previously postulated potential of the OMVs as adjuvant. Unfortunately, the antibody response raised did not translate into an effective protection against A. pleuropneumoniae infection, as none of the immunized groups following challenge showed a significantly lower degree of lesions than the controls. Interestingly, quite the opposite was true, as the animals with the highest IgG titers were also the ones bearing the most extensive lesions in their lungs. These results shed new light on A. pleuropneumoniae pathogenicity, suggesting that antibody-mediated cytotoxicity from the host immune response may play a central role in the development of the lesions typically associated with A. pleuropneumoniae infections.

show abstract

Section: Introductionmentioning

confidence: 99%

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Antenucci

Fougeroux

Deeney

et al. 2018

Vet Res

View full text Add to dashboard Cite

show abstract

“…The pangenome of six Brazilian APP8 isolates was recently reported (Prado et al, 2020) and showed that the gene repertoire is well conserved in relation to the available genomes of other serovars, though the presence of serovar-specific patterns of AMR within A. pleuropneumoniae is debatable (Asawa et al, 1995;Lee et al, 2015;Kim et al, 2016). Due to a low prevalence of clinical pleuropneumonia caused by APP8 globally, most studies on AMR have been performed on other serovars.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genome Sequence Analysis of Actinobacillus pleuropneumoniae Serovar 8 Isolates From Norway, Denmark, and the United Kingdom Indicates Distinct Phylogenetic Lineages and Differences in Distribution of Antimicrobial Resistance Genes

et al. 2021

View full text Add to dashboard Cite

Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a disease of major impact on pig health, welfare, and productivity globally. Serovar 8 (APP) is the predominant clinical serovar in Norway and the United Kingdom (UK), and has been isolated from clinical cases in Denmark. The primary objective of this study was to characterize the genetic variability of isolates of A. pleuropneumoniae APP8 in the Norwegian population. The secondary objectives were to determine the within-host variability of APP8; to compare the APP8 bacterial populations in Norway, Denmark, and the UK, including antimicrobial resistance (AMR) gene profiles and to assess the effect of national differences in antimicrobial drug use and restricted animal movement on the occurrence of resistance. Isolates of APP8 from the UK (n=67), Denmark (n=22), and Norway (n=123) collected between 1983 and 2020 were compared using whole genome sequencing. To investigate genetic variability within individual hosts, an additional 104 APP8 isolates from the lungs of six Norwegian pigs were compared. Very low within-host variation was observed (≤ 2 single nucleotide polymorphisms). The phylogeny of 123 Norwegian APP8 isolates from 76 herds revealed some within-herd genetic variation, but substantial geographical clustering. When inferring the relatedness of the three international APP8 collections, the topology highlighted the existence of two distinct monophyletic branches characterized by the Norwegian and UK isolates, respectively. Three Danish isolates were scattered across the UK branch, whereas the remaining 19 Danish isolates clustered in two monophyletic groups nested in the Norwegian branch. Coalescence analysis, performed to estimate the divergences from a common ancestor, indicated a last common ancestor several centuries ago. The phylogenetic analyses also revealed striking differences in occurrence of AMR genes, as these were 23-times more prevalent among the UK isolates than among the Norwegian isolates. An increased understanding of the effects of population strategies is helpful in surveillance and control of infectious diseases.

show abstract

“…This study used non-probabilistic sampling obtained from data of a private laboratory. One hundred sixty bacterial strains isolated from pigs with clinical respiratory signs from 51 pig farms in Brazil were studied, as follows: P. multocida [78/160 (48.75%) Lönnqvist et al (2018), and Kim et al (2016). The breakpoints used are detailed in the supplementary material.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance in bacteria isolated from pigs with respiratory clinical signs in Brazil

Gonçalves¹,

Nascimento²,

Alves³

et al. 2020

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Antimicrobial resistance is a current and important issue to public health, and it is usually associated with the indiscriminate use of antimicrobials in animal production. This study aimed to evaluate the antimicrobial susceptibility profile in bacterial isolates from pigs with clinical respiratory signs in Brazil. One hundred sixty bacterial strains isolated from pigs from 51 pig farms in Brazil were studied. In vitro disk-diffusion method was employed using 14 antimicrobial agents: amoxicillin, penicillin, ceftiofur, ciprofloxacin, enrofloxacin, chlortetracycline, doxycycline, oxytetracycline, tetracycline, erythromycin, tilmicosin, florfenicol, lincomycin, and sulfadiazine/trimethoprim. The majority of isolates were resistant to at least one antimicrobial agent (98.75%; 158/160), while 31.25% (50/160) of the strains were multidrug resistant. Streptococcus suis and Bordetella bronchiseptica were the pathogens that showed higher resistance levels. Haemophilus parasuis showed high resistance levels to sulfadiazine/trimethoprim (9/18=50%). We observed that isolates from the midwestern and southern regions exhibited four times greater chance of being multidrug resistant than the isolates from the southeastern region studied. Overall, the results of the present study showed a great level of resistance to lincomycin, erythromycin, sulfadiazine/trimethoprim, and tetracycline among bacterial respiratory pathogens isolated from pigs in Brazil. The high levels of antimicrobial resistance in swine respiratory bacterial pathogens highlight the need for the proper use of antimicrobials in Brazilian pig farms.

show abstract

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Cited by 27 publications

References 27 publications

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Comparative Genome Sequence Analysis of Actinobacillus pleuropneumoniae Serovar 8 Isolates From Norway, Denmark, and the United Kingdom Indicates Distinct Phylogenetic Lineages and Differences in Distribution of Antimicrobial Resistance Genes

Antimicrobial resistance in bacteria isolated from pigs with respiratory clinical signs in Brazil

Contact Info

Product

Resources

About