Molecular simulations guide immobilization of lipase on nest-like ZIFs with regulatable hydrophilic/hydrophobic surface

Zhong, Le; Wang, Zhongjie; Ye, Xiaohong; Cui, Jiandong; Wang, Ziyuan; Jia, Shiru

doi:10.1016/j.jcis.2024.04.075

Cited by 18 publications

(2 citation statements)

References 80 publications

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“…To test the immobilization efficiency and recovery of urease activity, we measured the concentration of urease protein in the initial urease solution and supernatant washing solution using the BCA kit as well as enzyme activity of the initial urease solution and composites. The immobilized urease efficiency (%), immobilized capacity (g/g) and immobilized urease activity recovery (%) were calculated by the following equation: , Immobilized urease efficiency false( % false) = true( m normali − m normals m normali true) × 100 Immobilized urease capacity false( normalg / normalg false) = m i − m s W …”

Section: Methodsmentioning

confidence: 99%

Immobilized Urease Vector System Based on the Dynamic Defect Regeneration Strategy for Efficient Urea Removal

Wang,

Chen

et al. 2024

ACS Appl. Mater. Interfaces

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The clearance of urea poses a formidable challenge, and its excessive accumulation can cause various renal diseases. Urease demonstrates remarkable efficacy in eliminating urea, but cannot be reused. This study aimed to develop a composite vector system comprising microcrystalline cellulose (MCC) immobilized with urease and metal−organic framework (MOF) UiO-66-NH 2 , denoted as MCC@UiO/U, through the dynamic defect generation strategy. By utilizing competitive coordination, effective immobilization of urease into MCC@UiO was achieved for efficient urea removal. Within 2 h, the urea removal efficiency could reach up to 1500 mg/g, surpassing an 80% clearance rate. Furthermore, an 80% clearance rate can also be attained in peritoneal dialyzate from patients. MCC@UiO/U also exhibits an exceptional bioactivity even after undergoing 5 cycles of perfusion, demonstrating remarkable stability and biocompatibility. This innovative approach and methodology provide a novel avenue and a wide range of immobilized enzyme vectors for clinical urea removal and treatment of kidney diseases, presenting immense potential for future clinical applications.

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Section: Methodsmentioning

confidence: 99%

Immobilized Urease Vector System Based on the Dynamic Defect Regeneration Strategy for Efficient Urea Removal

Wang,

Chen

et al. 2024

ACS Appl. Mater. Interfaces

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“…Furthermore, the performance of immobilized lipases is highly correlated with the structure and properties of the carrier . In general, hydrophobic materials with a high specific surface area are considered to be ideal carriers for lipase immobilization, allowing high immobilization efficiency as well as high activity. − In addition, efficient immobilization strategies and the addition of suitable additives also greatly improved the activity and stability of the immobilized lipase. − Considering the facile de novo design and postsynthetic modification of MOFs, the immobilization of lipases on MOFs deserves further investigation to facilitate their broader application.…”

Section: Introductionmentioning

confidence: 99%

Single-Crystalline Ordered Nanoporous Metal–Organic Frameworks for Lipase Immobilization and Structured Lipid Production

Liu,

Li,

Dai

et al. 2024

ACS Appl. Nano Mater.

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Single-crystalline ordered macro-microporous CuBTC shows excellent mass transfer performance and has high fatty acid stability, making it highly promising as an enzyme immobilization carrier. However, its practical application is severely limited by its poor water stability. To address this challenge, we introduced a ligand, 1,2,3-benzotriazole (BTA), known for its hydrophobicity and strong coordination with copper. A series of CuBTCderived dual-ligand SOM-MOFs (SOM-MIXs) was successfully synthesized with enhanced hydrophobicity and improved water stability. The CuBTCderived SOM-MIXs demonstrated superior performance for lipase immobilization, resulting in a maximum increase of 66.9% in specific activity. To address the limitation that excessive addition of 1,2,3-benzotriazole does not further enhance the hydrophobicity of the carrier and may lead to structural damage, a sol−gel method was employed to coat the SOM-MIX with highly hydrophobic PDMS, leading to a further increase of 139.1% in the specific activity. The resulting immobilized lipase exhibited excellent catalytic performance and remarkable reusability, with 90.09% activity retention after five cycles in the synthesis process of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) by acidolysis. This work highlights the potential of SOM-MIX@PDMS and provides valuable insights into the rational design and postmodification of metal−organic frameworks (MOFs) for enzyme immobilization in diverse applications.

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Bioremediation of organic pollutants by laccase-metal–organic framework composites: A review of current knowledge and future perspective

Aghaee,

Salehipour,

Rezaei

et al. 2024

Bioresource Technology

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Molecular simulations guide immobilization of lipase on nest-like ZIFs with regulatable hydrophilic/hydrophobic surface

Cited by 18 publications

References 80 publications

Immobilized Urease Vector System Based on the Dynamic Defect Regeneration Strategy for Efficient Urea Removal

Immobilized Urease Vector System Based on the Dynamic Defect Regeneration Strategy for Efficient Urea Removal

Single-Crystalline Ordered Nanoporous Metal–Organic Frameworks for Lipase Immobilization and Structured Lipid Production

Bioremediation of organic pollutants by laccase-metal–organic framework composites: A review of current knowledge and future perspective

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