Molecular Species Delimitation of Icelandic Brittle Stars (Ophiuroidea)

Khodami, Sahar; Arbizu, Pedro Martínez; Stöhr, Sabine; Laakmann, Silke

doi:10.2478/popore-2014-0011

Cited by 18 publications

(22 citation statements)

References 48 publications

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“…Similar to the Australian findings [44], our results revealed the highest intra-specific variability in ophiuroid echinoderms. This variability within the North Sea brittle star species is similarly high as that revealed from the COI analysis from Icelandic waters (11 species, 66 specimens [47]). …”

Section: Identification Of North Sea Echinodermssupporting

confidence: 57%

“…Most of the comprehensive molecular genetic biodiversity studies on echinoderms were conducted off the coasts of southern and western Australia and New Zealand [44], in the coastal waters of Canada [45] and in the Arctic [46]. Ophiuroidea are studied in Icelandic waters [47]. In most of these biodiversity studies, mitochondrial DNA, mainly a fragment of the mitochondrial cytochrome c oxidase subunit I (COI), was analysed.…”

Section: Open Accessmentioning

confidence: 99%

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Species identification of echinoderms from the North Sea by combining morphology and molecular data

et al. 2016

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Background: Taxonomic uncertainties in the morphological species identification and taxonomic revisions in individual groups are known for all echinoderm classes. These uncertainties in morphological species identification and discrimination have spawned the application of molecular genetic identification techniques. However, as the fundamental step to allow and ensure future molecular species identification, valid and comprehensive reference library entries comprising morphological and molecular species information together with various metadata are essentially needed. In our study we compare morphological and molecular genetic species identification techniques for representatives of North Sea echinoderm classes, i.e. the Asteroidea, Ophiuroidea, Echinoidea and Holothuroidea.Methods: Individuals were sampled during different surveys in different regions of the North Sea, identified to species level based on morphological diagnostic features, and were genetically analysed using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI). Results and Discussion:The morphological determination revealed 32 species including one taxon determined only to genus level. In contrast to this, the COI analysis supported 34 monophyletic clades with pronounced differences between the intra-and the inter-specific genetic variability (a barcoding gap of 4.93 %) with highest intra-specific variabilities found in the ophiuroid species Amphiura filiformis, A. chiajei and Ophiura sarsii. In 94 % of the investigated species, morphological identification and COI sequence clusters were congruent whereas for two asteroid species we found an underestimated diversity. For Astropecten irregularis, one of the most common starfish species of the North Sea, we found two distinct and possibly depth-related clades, probably sibling species, differing by 11.1-11.9 % sequence divergences (p-distances). For two starfish individuals, morphologically identified as Henricia sanguinolenta, the COI analysis revealed two monophyletic clades, of which one was classified as H. cf. oculata by comparison to published sequences. Conclusions:This newly established sequence reference library for the North Sea Echinodermata allows and ensures future molecular species identification for various life-cycle stages including juveniles and meroplanktonic larvae and provides sequences for phylogeographic studies and the detection of sibling as well as cryptic species.

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Section: Identification Of North Sea Echinodermssupporting

confidence: 57%

Section: Open Accessmentioning

confidence: 99%

Species identification of echinoderms from the North Sea by combining morphology and molecular data

et al. 2016

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“…The Icelandic marine Animals Genetic and Ecology (IceAGE) project aimed to understand how underwater physical structures (e.g., submerged ridges) and non-physical barriers (e.g., currents, temperature, salinity) affect the distribution of benthic organisms (Brix et al 2014). Traditional taxonomic methods as well as modern approaches to biodiversity research (ecological modeling and molecular species discrimination) have been studied for such groups as Isopoda, Tanaidacea, Ophiuroidea, and Mollusca (Brix, 2011;Błażewicz-Paszkowycz et al 2014;Khodami et al 2014;Mikkelsen and Todt 2014;Todt and Kocot 2014). Benthic samples collected from an extensive depth range (117−2750 m), at different localities around Iceland, providing an opportunity to test if, and to what extent, topographic and oceanographic barriers (i.e., ridges, currents) influence the distribution, community structure, and diversity of benthic organisms.…”

Section: Introductionmentioning

confidence: 99%

A tip of the iceberg—Pseudotanaidae (Tanaidacea) diversity in the North Atlantic

2018

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During two IceAGE expeditions, a large collection of Tanaidacea was gathered from the shelf down to the slope (213−2750 m) in six areas off Iceland-the Irminger Basin, the Iceland Basin, the Norwegian Sea, the Denmark Strait, the Iceland-Faroe Ridge, and the Norwegian Channel. In this collection, members of the family Pseudotanaidae were most numerous component. We examined 40 samples collected with different gears (e.g., EBS, VVG. GKG), in which 323 pseudotanaid individuals were counted and covered a total depth from 213.9 to 2746.4 m. Morphological identification of the material has revealed the presence of five species: Akanthinotanais cf. longipes, Mystriocentrus biho sp. n. Pseudotanais misericorde sp. n., P. svavarssoni sp. n., and P. sigrunis sp. n. The description of the four new species has been presented in the paper and a rank of the subgenus Akanthinotanais is elevated to a genus rank. A large group of morphologically almost identical specimens, similar with P. svavarssoni sp. n. from a wide depth range and from various areas off Iceland was discriminated to species by applying morphometric methods; one distinct species (P. svavarssoni sp. n.) and complex of presumably cryptic species the species was discovered. Based on current data and literature records, similarity among fauna of Pseudotanaidae was assessed with applying Bray-Curtis formula. As results, potential zoogeographic regions in the North Atlantic have been distinguished.

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“…Several resulting publications were compiled in the first IceAGE special issue published by Polish Polar Research in 2014. It included many descriptions of newly discovered species combined with taxonomic and biodiversity studies (Jóźwiak 2014;Mikkelsen and Todt 2014;Parapar et al 2014;Schnurr and Malyutina 2014;Todt and Kocot 2014;Yasuhara et al 2014), studies combining taxonomy and phylogeny using morphological and molecular tools (Błażewicz-Paszkowycz et al 2014;Khodami et al 2014), phylogeographic studies on selected taxa (Brix et al 2014b;Jennings and Etter 2014), and a publication with a modeling approach providing a GIS layer on sediment characteristics .…”

mentioning

confidence: 99%