Molecular species delimitation reveals high diversity in the mosquito Anopheles tessellatus Theobald, 1901 (Diptera, Culicidae) across its range

Bourke, Brian P.; Wilkerson, Richard C.; Linton, Yvonne‐Marie

doi:10.1016/j.actatropica.2020.105799

Cited by 7 publications

(154 citation statements)

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“…tessellatus species A and C. Specimens from Nan province, Northern Thailand, were grouped into An . tessellatus species C, which is consistent with previous studies that described that they are widely spread in mainland Southeast Asia, such as China and Laos, a territory near Northern Thailand [ 78 ]. One specimen from the Phang Nga province, Southern Thailand, was grouped into An .…”

Section: Discussionsupporting

confidence: 91%

“…One specimen from the Phang Nga province, Southern Thailand, was grouped into An. tessellatus species A, which is consistent with previous studies that found that this species was distributed in Singapore, where it was nearly located in the Southern region of Thailand [78].…”

Section: Plos Onesupporting

confidence: 92%

“…A recent study reported that the An . tessellatus complex includes at least six sibling species: A, B, C, D, E, and F [ 78 ]. In addition, these species can transmit Plasmodium parasites in Sumatra, Indonesia [ 79 ], and Sri Lanka [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

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Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

et al. 2022

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The correct identification of mosquito species is important for effective mosquito vector control. However, the standard morphological identification of mosquito species based on the available keys is not easy with specimens in the field due to missing or damaged morphological features during mosquito collections, often leading to the misidentification of morphologically indistinguishable. To resolve this problem, we collected mosquito species across Thailand to gather genetic information, and evaluated the DNA barcoding efficacy for mosquito species identification in Thailand. A total of 310 mosquito samples, representing 73 mosquito species, were amplified using mitochondrial cytochrome c oxidase subunit I (COI) primers. The average maximum intraspecific genetic variation of the 73 mosquito species was 1% ranged from 0–5.7%. While, average minimum interspecific genetic variation (the distance to the nearest neighbour) of the 73 mosquito species was 7% ranged from 0.3–12.9%. The identification of success rates based on the “Best Match,” “Best Close Match,” and “All Species Barcodes” methods were 97.7%, 91.6%, and 81%, respectively. Phylogenetic analyses of Anopheles COI sequences demonstrated a clear separation between almost all species (except for those between An. baimaii and An. dirus), with high bootstrap support values (97%–99%). Furthermore, phylogenetic analyses revealed potential sibling species of An. annularis, An. tessellatus, and An. subpictus in Thailand. Our results indicated that DNA barcoding is an effective molecular approach for the accurate identification of mosquitoes in Thailand.

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Section: Discussionsupporting

confidence: 91%

Section: Plos Onesupporting

confidence: 92%

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Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

et al. 2022

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“…tessellatus An. tessellatus 97.7 Y OL352204 Bourke et al [ 44 ] The percentage of matching bases from the BLAST is given for the first shown result. Matching success of the two methods for species identification is 40% …”

Section: Resultsmentioning

confidence: 99%

“…when different species matched our sequences equally well), against the BOLD database. As an alternative identification approach, sequences (preferably those that were verified by morphological identification [ 44 – 49 ]) of species, that are common to the region from which the samples originated were downloaded from the NCBI database (for accession numbers, refer to Fig. 2 , Additional file 1 : Fig.…”

Section: Methodsmentioning

confidence: 99%

A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country

et al. 2022

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Background Vector-borne diseases are on the rise on a global scale, which is anticipated to further accelerate because of anthropogenic climate change. Resource-limited regions are especially hard hit by this increment with the currently implemented surveillance programs being inadequate for the observed expansion of potential vector species. Cost-effective methods that can be easily implemented in resource-limited settings, e.g. under field conditions, are thus urgently needed to function as an early warning system for vector-borne disease epidemics. Our aim was to enhance entomological capacity in Nepal, a country with endemicity of numerous vector-borne diseases and with frequent outbreaks of dengue fever. Methods We used a field barcoding pipeline based on DNA nanopore sequencing (Oxford Nanopore Technologies) and verified its use for different mosquito life stages and storage methods. We furthermore hosted an online workshop to facilitate knowledge transfer to Nepalese scientific experts from different disciplines. Results The use of the barcoding pipeline could be verified for adult mosquitos and eggs, as well as for homogenized samples, dried specimens, samples that were stored in ethanol and frozen tissue. The transfer of knowledge was successful, as reflected by feedback from the participants and their wish to implement the method. Conclusions Cost effective strategies are urgently needed to assess the likelihood of disease outbreaks. We were able to show that field sequencing provides a solution that is cost-effective, undemanding in its implementation and easy to learn. The knowledge transfer to Nepalese scientific experts from different disciplines provides an opportunity for sustainable implementation of low-cost portable sequencing solutions in Nepal. Graphical Abstract

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Entomology Monographs

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Molecular species delimitation reveals high diversity in the mosquito Anopheles tessellatus Theobald, 1901 (Diptera, Culicidae) across its range

Cited by 7 publications

References 56 publications

Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country

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