Molecular spectroscopy studies of solvent properties of dispersed ‘water pools’: Fluorescein and 2,7-dichlorofluorescein in reversed AOT-based microemulsions

Vodolazkaya, Natalya A.; Mchedlov‐Petrossyan, Nikolay O.; Salamanova, Natalya V.; Surov, Yuriy N.; Дорошенко, А. О.

doi:10.1016/j.molliq.2010.08.013

Cited by 25 publications

(33 citation statements)

References 64 publications

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“…The actual standard definition for pH, recommended by the International Union of Pure and Applied Chemistry (IU-PAC), is based on hydronium ion concentrations and is applied only for a range of pH between 2 and 12, at an ionic strength of no more than 0.1 mol•L −1 [86]. According to these conditions, the minimum volume necessary to apply the conventional definition of pH in an aqueous solvent is determined by the dissociation of water molecules [87,88]. For instance, at neutral pH, only one in 10 7 molecules of water is dissociated; however, the number of water molecules in very large RMs is only about 300,000 (w0 = 40), with this number being even lower in smaller RMs (e.g., about 30.8 molecules for w0 = 1.5 or 20,500 molecules for w0 = 10.5) [89].…”

Section: Ph Across the Water Poolmentioning

confidence: 99%

Versatility of Reverse Micelles: From Biomimetic Models to Nano (Bio)Sensor Design

et al. 2021

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This paper presents an overview of the principal structural and dynamics characteristics of reverse micelles (RMs) in order to highlight their structural flexibility and versatility, along with the possibility to modulate their parameters in a controlled manner. The multifunctionality in a large range of different scientific fields is exemplified in two distinct directions: a theoretical model for mimicry of the biological microenvironment and practical application in the field of nanotechnology and nano-based sensors. RMs represent a convenient experimental approach that limits the drawbacks of the conventionally biological studies in vitro, while the particular structure confers them the status of simplified mimics of cells by reproducing a complex supramolecular organization in an artificial system. The biological relevance of RMs is discussed in some particular cases referring to confinement and a crowded environment, as well as the molecular dynamics of water and a cell membrane structure. The use of RMs in a range of applications seems to be more promising due to their structural and compositional flexibility, high efficiency, and selectivity. Advances in nanotechnology are based on developing new methods of nanomaterial synthesis and deposition. This review highlights the advantages of using RMs in the synthesis of nanoparticles with specific properties and in nano (bio)sensor design.

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Section: Ph Across the Water Poolmentioning

confidence: 99%

Versatility of Reverse Micelles: From Biomimetic Models to Nano (Bio)Sensor Design

et al. 2021

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“…So, many researchers have studied properties of reverse micellar core using dye molecules [57,58]. MB is a well known cationic dye in this context and it can form aggregates in polar medium.…”

Section: Visible Spectroscopy Study With Mbmentioning

confidence: 99%

Comparative studies on brij reverse micelles prepared in benzene/surfactant/ethylammonium nitrate systems: Effect of head group size and polarity of the hydrocarbon chain

Ghosh

2011

Journal of Colloid and Interface Science

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“… 28 , 29 A number of studies have been undertaken to experimentally characterize pH within reverse micelle water cores including the use of oxovanadate probes with 51 V NMR, 27 , 30 − 33 phosphate and pyrophosphate with 31 P NMR, 29 , 34 measurements of water T 2 relaxation times with proton NMR, 35 and hydroxypyrene trisulfonate and fluorescein measurements using optical spectroscopy. 26 , 36 …”

Section: Introductionmentioning

confidence: 99%

Measurement and Control of pH in the Aqueous Interior of Reverse Micelles

et al. 2014

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The encapsulation of proteins and nucleic acids within the nanoscale water core of reverse micelles has been used for over three decades as a vehicle for a wide range of investigations including enzymology, the physical chemistry of confined spaces, protein and nucleic acid structural biology, and drug development and delivery. Unfortunately, the static and dynamical aspects of the distribution of water in solutions of reverse micelles complicate the measurement and interpretation of fundamental parameters such as pH. This is a severe disadvantage in the context of (bio)chemical reactions and protein structure and function, which are generally highly sensitive to pH. There is a need to more fully characterize and control the effective pH of the reverse micelle water core. The buffering effect of titratable head groups of the reverse micelle surfactants is found to often be the dominant variable defining the pH of the water core. Methods for measuring the pH of the reverse micelle aqueous interior using one-dimensional 1H and two-dimensional heteronuclear NMR spectroscopy are described. Strategies for setting the effective pH of the reverse micelle water core are demonstrated. The exquisite sensitivity of encapsulated proteins to the surfactant, water content, and pH of the reverse micelle are also addressed. These results highlight the importance of assessing the structural fidelity of the encapsulated protein using multidimensional NMR before embarking upon a detailed structural and biophysical characterization.

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Molecular spectroscopy studies of solvent properties of dispersed ‘water pools’: Fluorescein and 2,7-dichlorofluorescein in reversed AOT-based microemulsions

Cited by 25 publications

References 64 publications

Versatility of Reverse Micelles: From Biomimetic Models to Nano (Bio)Sensor Design

Versatility of Reverse Micelles: From Biomimetic Models to Nano (Bio)Sensor Design

Comparative studies on brij reverse micelles prepared in benzene/surfactant/ethylammonium nitrate systems: Effect of head group size and polarity of the hydrocarbon chain

Measurement and Control of pH in the Aqueous Interior of Reverse Micelles

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