Molecular strategy for identification in<i>Aspergillus</i> section<i>Flavi</i>

Godet, Marie; Munaut, Françoise

doi:10.1111/j.1574-6968.2009.01890.x

Cited by 61 publications

(39 citation statements)

References 37 publications

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“…We did not specify any species from these sections because the applied methods were sufficient only for sectional classifications. Due to morphological similarities among species from each section, specific DNA sequence data is required (16,(34)(35)(36)(37)(38).…”

Section: Resultsmentioning

confidence: 99%

A year-round investigation of indoor airborne fungi in Croatia

Despot

Klarić

2014

Archives of Industrial Hygiene and Toxicology

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This study assessed the composition of aeromycota at a grain mill and four dwellings (two apartments and two basements) as well as in outdoor air during one year in Zagreb, Croatia. The incidence of Aspergilli from sections Flavi, Nigri, and Versicolores was also assessed. Airborne fungi were collected using an air-sampler and DG-18 agar plates. The average concentrations of airborne fungi in the grain mill ranged from 14,310 to 40,000 cfu m -3 , which was above the hazardous level (10 4 cfu m -3 ), whereas the values statistically estimated using Feller's correction were up to six times higher. Concentrations in the apartment (163-1244 cfu m ), except in the warmer period of the year when they were similar. The most abundant species throughout the year were Cladosporium spp. (90-100 %), Penicillium spp. (40-100 %), and Alternaria spp. (10-100 %), which are common for temperate climates. Aspergilli from the Flavi (50-100 %) and Nigri (15-40 %) sections as well as A. ochraceus (15-60 %) and Eurotium spp. (85-100 %) were the most abundant at the grain mill and were rarely found in outdoor air. In the basement, Aspergilli (Versicolores) were more abundant than in the apartment. The excess of aeromycoparticles in the grain mill throughout the year may have represented a serious health risk to mill workers. This is the first Croatian one-year study of indoor airborne fungi in a grain mill and dwellings; however monitoring should continue over a longer period.

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Section: Resultsmentioning

confidence: 99%

A year-round investigation of indoor airborne fungi in Croatia

Despot

Klarić

2014

Archives of Industrial Hygiene and Toxicology

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“…1 Aspergillus oryzae and A. sojae are classified in the Aspergillus section Flavi , which also includes plant pathogen A. flavus and A. parasiticus that produces aflatoxins known to be carcinogenic substances. The analysis based on restriction-site polymorphisms of genes coding for 11 proteins and sequences of five of those genes suggested that A. oryzae is a species derived from A. flavus through human handling.…”

Section: Introductionmentioning

confidence: 99%

Draft Genome Sequencing and Comparative Analysis of Aspergillus sojae NBRC4239

et al. 2011

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We conducted genome sequencing of the filamentous fungus Aspergillus sojae NBRC4239 isolated from the koji used to prepare Japanese soy sauce. We used the 454 pyrosequencing technology and investigated the genome with respect to enzymes and secondary metabolites in comparison with other Aspergilli sequenced. Assembly of 454 reads generated a non-redundant sequence of 39.5-Mb possessing 13 033 putative genes and 65 scaffolds composed of 557 contigs. Of the 2847 open reading frames with Pfam domain scores of >150 found in A. sojae NBRC4239, 81.7% had a high degree of similarity with the genes of A. oryzae. Comparative analysis identified serine carboxypeptidase and aspartic protease genes unique to A. sojae NBRC4239. While A. oryzae possessed three copies of α-amyalse gene, A. sojae NBRC4239 possessed only a single copy. Comparison of 56 gene clusters for secondary metabolites between A. sojae NBRC4239 and A. oryzae revealed that 24 clusters were conserved, whereas 32 clusters differed between them that included a deletion of 18 508 bp containing mfs1, mao1, dmaT, and pks-nrps for the cyclopiazonic acid (CPA) biosynthesis, explaining the no productivity of CPA in A. sojae. The A. sojae NBRC4239 genome data will be useful to characterize functional features of the koji moulds used in Japanese industries.

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“…It should be pointed out that the identification of new species and restriction of the taxon in new groups are the result of a polyphasic taxonomic approach (Samson, Hong, & Frisvad, 2006;Klich, 2007;Godet & Munaut, 2010).…”

Section: Aflatoxins In Brazil Nutsmentioning

confidence: 99%