Molecular, structural, and phylogenetic characterization of two chlorophyllase isoforms in Pachira macrocarpa

Chen, Miles Chih-Ming; Yang, Jay-How; Liu, Chiao-Hui; Lin, Kuan‐Hung; Yang, Chi-Ming

doi:10.1007/s00606-013-0908-5

Cited by 16 publications

(16 citation statements)

References 44 publications

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“…Meanwhile, expression of CLHs, regardless of involvement in Chl degradation, also affect leaf Chl contents and color (Takamiya et al 2000;Eckhardt et al 2004;Hörtensteiner 2006;Harpaz-Saad et al 2007;Liao et al 2007;Chen et al 2014). As shown in the present study, CLHs (ApCLH1 and ApCLH2) each had one expression peak at 11 Apr.…”

Section: Gene Expression In a Palmatumsupporting

confidence: 69%

“…Some advanced technologies, such as whole genome sequencing and single molecule real-time sequencing for transcriptome, will be helpful to better understand the molecular regulatory mechanism of Chl biosynthesis in this species. Of course, the capacity for Chl breakdown is also an important factor affecting leaf color (Takamiya et al 2000;Eckhardt et al 2004;Hörtensteiner 2006;Harpaz-Saad et al 2007;Liao et al 2007;Shalygo et al 2009;Chen et al 2014;Lin et al 2014). In our experiment, two CLH genes (ApCLH1 and ApCLH2) were highly expressed in the yellow leaves of YM, accompanying the sharp decline in Chl content (Figs.…”

Section: Coloring Mechanism Of Ym Leavesmentioning

confidence: 54%

“…Mutations in structural genes in Chl biosynthesis or degradation can produce mutants with reduced or increased Chl contents (Tanaka and Tanaka 2005;Harpaz-Saad et al 2007;Liao et al 2007;Pattanayak et al 2007;Shalygo et al 2009;Paddock et al 2010Paddock et al , 2012Lin et al 2014). Additionally, appropriate agronomic practices can regulate leaf color by affecting expression of these genes (Harper et al 2004;Tanaka and Tanaka 2005;Nakagawara et al 2007;Pattanayak et al 2007;Chen et al 2014;Li et al 2015b).…”

Section: Coloring Mechanism Of Ym Leavesmentioning

confidence: 99%

“…Walp. (Chen et al 2014). Two AtCLHs exhibited differential expression patterns and the contents of Chlides a and b in the leaves of their RNA interference lines were substantially reduced (Liao et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, chlorophyllase (Chlase) was known as the first and main enzyme for Chl degradation, which removes hydrophobic phytol chains from Chl and forms Chlide (Takamiya et al 2000;Eckhardt et al 2004;Hörtensteiner 2006;Harpaz-Saad et al 2007;Barry 2009). Several studies have confirmed that two isoforms (CLH1 and CLH2) of the Chlase gene exist in many plants (Tsuchiya et al 1997;Okazawa et al 2006;Schenk et al 2007;Chen et al 2014). Despite the low homology of whole amino acid sequences among plant Chlases (Takamiya et al 2000), their encoding genes play a distinctive role in Chl catabolism in vivo (Liao et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum leaves

Tang

et al. 2017

Can. J. Plant Sci.

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Exterior leaf color is an important trait for ornamental foliage plants but the molecular mechanism underlying the variation in color between yellow-and green-leaf Acer palmatum Thunb. ex Murray is poorly understood. Indeed, chlorophyll (Chl) contents and gene transcriptions were very different between the yellow mutant (YM) and green wild-type (GW) leaves of this species. Here, annual variations of leaf color and pigment contents in two varieties were investigated. Then, transcript levels of six genes involved in Chl metabolism were quantitatively measured using real-time polymerase chain reaction in YM and GW leaves. Compared with GW, the color changes of YM leaves were classified into four typical periods. During the yellow leaf stage, YM showed greatly reduced Chl synthesis and a higher ratio of carotenoid (Car) to Chl than GW. These phenomena may be the main cause of yellow leaves in YM. In addition, expression levels of Chl synthesis genes (ApPORA, ApPORB, ApCAO, and ApCHLG) in YM were very low during this period. Concomitantly, the amount of transcripts of genes for Chl degradation (ApCLH1 and ApCLH2) rose significantly compared with GW. Once YM leaves turned red, the above differences between the two genotypes lessened. Overall, our results indicated that changes in Chl synthesis and degradation may play important roles leading to the yellow leaves of YM A. palmatum.

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Section: Gene Expression In a Palmatumsupporting

confidence: 69%

Section: Coloring Mechanism Of Ym Leavesmentioning

confidence: 54%

Section: Coloring Mechanism Of Ym Leavesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum leaves

Tang

et al. 2017

Can. J. Plant Sci.

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Chlorophyllase‐Catalyzed Chlorophyll Removal from Vegetable Oils Using Recombinant Eukaryotic and Prokaryotic Enzymes

Sharafi

Dehestani

Farmani

et al. 2021

J Americ Oil Chem Soc

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Chlorophyllase converts chlorophyll and pheophytin into their colorless derivatives (chlorophyllide/ pheophorbide and phytol). This activity can be used in chlorophyll removal from vegetable oils. Chlorophyllase genes from Oscillatoria acuminata (OscChlase) and Citrus aurantium (CitChlase) were isolated, cloned, and expressed in E. coli. Bioinformatics analysis showed that both chlorophyllases shared a conserved GHSXG lipase motif responsible for their catalytic activity. SDS-PAGE and immunoblot assays revealed that both enzymes had a molecular weight of 35 kDa. The purified chlorophyllases were stable at a broad range of temperatures and showed the highest activity at 40 C. OscChlase and CitChlase exhibited the highest activity at pH 6.0 and 7.0, respectively. Enzyme kinetics analysis revealed that OscChlase was able to hydrolyze bacteriochlorophyll-a more efficiently than the recombinant CitChlase (Vmax/Km of 0.38 for OscChlase vs. 0.01 min −1 mg protein for CitChlase). Instead, CitChlase hydrolyzed chlorophyll-b more efficiently than OscChlase. Both enzymes were able to reduce the chlorophyll content of olive (from 623.1 to as low as 87.2 mg per kg oil) and canola oil (from 537.2 to as low as 101.1 mg per kg oil). The ratio of oil to the aqueous reaction media affected chlorophyll hydrolysis (P < 0.05). The lower the oil ratio was (10%), the higher the chlorophyll removal was (75-86%). The efficiency of CitChlase in chlorophyll removal was higher than that of OscChlase at oil ratios of 10 and 20, but lower at 30% ratio (P < 0.05). This is the first report on the application of recombinant OscChlase and CitChlase in chlorophyll removal (up to 86%) from vegetable oils.

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In Search of Engineered Prokaryotic Chlorophyllases: A Bioinformatics Approach

Sharafi

Farmani

Parizi

et al. 2018

Biotechnol Bioproc E

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Molecular, structural, and phylogenetic characterization of two chlorophyllase isoforms in Pachira macrocarpa

Cited by 16 publications

References 44 publications

Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum leaves

Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum leaves

Chlorophyllase‐Catalyzed Chlorophyll Removal from Vegetable Oils Using Recombinant Eukaryotic and Prokaryotic Enzymes

In Search of Engineered Prokaryotic Chlorophyllases: A Bioinformatics Approach

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