Molecular structure in peripheral dog breeds: Portuguese native breeds as a case study

Pires, Ana Elisabete; Amorim, Isabel R.; Ginja, Catarina; Gomes, Manuel; Godinho, I.; Simões, Fernanda; Oom, María do Mar; Petrucci-Fonseca, Francisco; Matos, José; Bruford, Michael William

doi:10.1111/j.1365-2052.2009.01849.x

Cited by 15 publications

(23 citation statements)

References 48 publications

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“…As a result of these mating practices, we found that 28% of the dogs with American pedigree were genetically classified as Europeans. Our findings are in agreement with recent studies, which support up to 30% of genetic variation in pure breeds [22]. This genetic variation can cause important genetic differences even among different countries as Quingnon and colleagues point out [23].…”

Section: Discussionsupporting

confidence: 94%

Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line

Noris¹,

Santana²,

Herrán-Aguirre³

et al. 2014

OJGen

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The American Kennel Club and the Fédération Cynologique Internationale recognize two phenotypic variants of Great Dane breed denominated American and European varieties. Historically, these varieties have been segregated according to morphological characteristics. In an attempt to obtain a better characterization, breeders have been interested in the genetic parameters that could evaluate the within-breed diversity. In this document, we studied the genetic structure of Great Dane breed with 10 STR markers in 88 dogs using capillary electrophoresis. Cluster analysis, population differentiation and phylogenetic analyses revealed that American and European varieties are genetically independent. Nevertheless, within the American variety a genetic stratification was found. Additionally, a high misclassification (28%) was detected, which could be due to wrong registration or false paternity. Our results support the importance to deem genetic markers as useful tools in breeding control. Similarly, these studies serve as an accurate reference to establish standards by dog breeding associations and for choosing among dogs for inter-breeding. Nevertheless, genetic tools are only a complement of morphological methods, since both are reshuffling * These authors equally contributed to this work. # Corresponding author. G. Noris et al. 79the control over the breeding line. Notwithstanding, this database provides an overall and scape concerning the impact of genetic diversity within-breed. Unmistakably, more databases are needed to increase the quality of the breeding line as well as the number of STR in order to study, with more detail, the genetic structure in the Great Dane race.

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Section: Discussionsupporting

confidence: 94%

Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line

Noris¹,

Santana²,

Herrán-Aguirre³

et al. 2014

OJGen

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“…Microsatellite and AFLP analyses of modern dog suggest that the genetic variability of dog breeds is structured according to breeds and geography (Pires et al, 2009) and that Portuguese dogs have multiple mtDNA haplotypes in agreement with the mtDNA diversity found in other European dogs (Pires et al, 2006).…”

supporting

confidence: 60%

“…In the Iberian Peninsula, both dog and wolf subspecies have been the focus of several studies where molecular markers were used to describe genetic composition, population structure, and phylogeny. Microsatellite and AFLP analyses of modern dog suggest that the genetic variability of dog breeds is structured according to breeds and geography (Pires et al., ) and that Portuguese dogs have multiple mtDNA haplotypes in agreement with the mtDNA diversity found in other European dogs (Pires et al., ).…”

Section: Introductionmentioning

confidence: 72%

New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula

Pires

Amorim

Borges

et al. 2017

Ecology and Evolution

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This study investigates the gene pool of Portuguese autochthonous dog breeds and their wild counterpart, the Iberian wolf subspecies (Canis lupus signatus), using standard molecular markers. A combination of paternal and maternal molecular markers was used to investigate the genetic composition, genetic differentiation and genetic rela- variants for this endemism. Our research also discloses new molecular markers for wolf and dog subspecies assignment, which might become particularly relevant in the case of forensic or noninvasive genetic studies. The Iberian wolf represents a relic of the once widespread wolf population in Europe and our study reveals that it is a reservoir of unique genetic diversity of the grey wolf, Canis lupus. These results stress the need for conservation plans that will guarantee the sustainability of this threatened top predator in Iberia.

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“…The Bayesian Clustering Algorithms can identify further substructure by re‐analysing the identified clusters following an approach used in Pires et al. (2009) and Bray et al.…”

Section: Methodsmentioning

confidence: 99%

Bayesian clustering techniques and progressive partitioning to identify population structuring within a recovering otter population in the UK

Hobbs

Chadwick

Bruford³

et al. 2011

Journal of Applied Ecology

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Summary1. After a major decline, the UK otter Lutra lutra population is now recovering in its known strongholds (northern England, Wales and Borders and southwest England) and also in central England where the population had become small, fragmented and was reinforced with captive bred individuals. Bayesian clustering and GIS are used here to identify the genetic structure of the UK otter population and to assess expansion from strongholds and the contribution of introduced otters. Large carnivores have recently started to recolonize landscapes where they were formerly absent, especially in developed countries and understanding the expansion of these populations is essential for informing conservation management, linking fragmented populations and re-establishing gene flow. 2. Three Bayesian clustering techniques were used (structure, geneland spatial and baps4 spatial) to estimate the number of otter populations (K). A novel progressive partitioning approach was tested to identify genetic substructuring at various hierarchical levels using successive partitions at K = 2. 3. Four regional populations were identified that reflect known population history. Isolated populations in southwest England and in Wales and its borders showed the lowest levels of genetic diversity. Higher diversity and private alleles in northern and central England reflect the proximity to genetically diverse Scottish populations and the positive effect of reintroductions. 4. Progressive partitioning was used to produce a more detailed analysis, by allowing comparison and combination of clusters identified by different techniques and by avoiding the subjective estimation and choice of K. 5. Synthesis and applications. Although the otter population is increasing, our data show little sign of population expansion from the stronghold regions into central England, instead reflecting the success of population reinforcement in this area. Our progressive partitioning approach allows the identification of fine-scale substructure (11 groups) that enables the prioritization of management effort including identifying barriers to dispersal within and between populations and monitoring of introduced individuals.

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Molecular structure in peripheral dog breeds: Portuguese native breeds as a case study

Cited by 15 publications

References 48 publications

Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line

Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line

New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula

Bayesian clustering techniques and progressive partitioning to identify population structuring within a recovering otter population in the UK

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