Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii

Hassani, M. E.; Gianibelli, M.C.; Shariflou, M. R.; Sharp, P. J.

doi:10.1007/s10681-005-6982-1

Cited by 15 publications

(7 citation statements)

References 37 publications

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“…Insertion in the central repetitive domain could directly affect the functional properties [47], suggesting that the extra 102 amino acid residue insertion in the central repetitive domain of 1S l ×2.3* subunit may play a positive role in dough visco-elastic properties. D'Ovidio and Anderson [48] suggested that y-type HMW-GS are among the main components responsible for differences in the technological characteristics of flour.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of HMW Glutenin Subunits from 1Sl Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality

Wang

Cao

et al. 2013

PLoS ONE

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A wheat cultivar “Chinese Spring” chromosome substitution line CS-1Sl(1B), in which the 1B chromosome was substituted by 1Sl from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1Sl×2.3* and 1Sly16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1Sl, making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat.

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of HMW Glutenin Subunits from 1Sl Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality

Wang

Cao

et al. 2013

PLoS ONE

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“…It is reasonable to hypothesize after duplication, these three alleles differentiated through unequal crossover or slippage to insert or delete blocks of repeats. Such mechanisms have been reported in the generation of several novel HMW-GS genes (Hassani et al 2005;Liu et al 2007). Considering the large number and extensive variations of Glu-3 alleles compared to Glu-1 loci, we can speculate that the unequal crossing over is an important pathway for enriching both copy numbers and variations of Glu-3 alleles.…”

Section: Discussionmentioning

confidence: 79%

Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution

et al. 2014

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Four low-molecular-weight-isoleucine (LMW-i)-type and one novel chimeric (between LMW-i and LMW-methionine (m) types) low-molecular-weight glutenin subunit (LMW-GS) genes were characterized from wild emmer wheat (Triticum dicoccoides), designated as emmer-1 to emmer-5. All five LMW-GS genes possessed the same primary structure shared by other published LMW-GSs. The three genes emmer-1, emmer-3, and emmer-5 are similar, with the exception that emmer-3 and emmer-5 lost a few repeat motifs compared to emmer-1. Gene duplication and insertions/deletions of repeat motifs mediated through unequal crossing over may be responsible for the generation of these three Glu-3 alleles. Although the first residue of mature peptide of emmer-4 is isoleucine, it is not typical LMW-i-type LMW-GS. Phylogenetic analysis indicated that emmer-4 is located in the LMW-m subgroup, suggesting a closer relationship with LMW-m-type gene Y14104 of T. durum. Sequence alignment indicated that the emmer-4 is likely a chimeric gene generated by illegitimate recombination between LMW-i and LMW-m type. Unequal crossing over and illegitimate recombination are effective mechanisms for enriching both copy numbers and variations of LMW-GSs.

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“…In general, longer and more regular repeating units have a positive effect on the elasticity and viscosity of the dough through intermolecular interactions (Masci et al 2000). The insertion of the intermediate repeat region fragments directly affects the function of glutenins (Hassani et al 2005). Compared with other HMW-GS, 1S l 2x, 1S l 16x, and 1S l 25x have 84 and 20 aa insertions at positions 669-752 and 778-797, respectively (Table 3), and both 1S l 6y 1 and 1S l 23y have one 15 aa insertion at the position 454-468 (Table 4), significantly increasing the length of the repeat region.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of special HMW glutenin subunit genes from Aegilops longissima L. and their potential for wheat quality improvement

Wang

Deng

et al. 2019

3 Biotech

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Identification and cloning of new glutenin genes from wheat-related species can provide candidate gene resources for quality improvement of wheat. In this study, ten special high-molecular-weight glutenin subunits (HMW-GS), including five x-type (1S l 2x, 1S l 16x, 1S l 17x, 1S l 23x, and 1S l 25x) and five y-type (1S l 2y, 1S l 6y 1 , 1S l 16y, 1S l 17y, and 1S l 23y) from Aegilops longissima L. (S l S l , 2n = 2x = 14) were identified, and their complete encoding genes were cloned by allelic-specific polymerase chain reaction (AS-PCR). The deduced amino acid (aa) residues of the x-type subunit genes ranged from 821 aa (2469 bp) to 941 aa (2829 bp), while those of the y-type subunit genes varied from 749 aa (2250 bp) to 771 aa (2361 bp). These special HMW-GS had a longer central repetitive domain with more glutamine repeats and glutamine residues compared to the previously characterized HMW-GS in common wheat, which provided a structural basis for superior gluten quality formation. The authenticity of the four cloned genes were verified by matrix-assisted laser desorption ionization time-of-flight/ time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Abundant single-nucleotide polymorphism (SNP) and insertion/ deletion (InDel) variations among these genes were identified, which would benefit for developing specific molecular markers used for wheat gluten quality improvement. Phylogenetic analysis revealed that the 1S l-encoded HMW-GS had close relationships with those from bread wheat, which were divergent from Triticum species at 2.10-10.00 million years ago. Our results indicate that the 1S l genome contains superior candidate glutenin genes that have potential application values for the improvement of wheat bread making quality.

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Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii

Cited by 15 publications

References 37 publications

Molecular Mechanisms of HMW Glutenin Subunits from 1Sl Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality

Molecular Mechanisms of HMW Glutenin Subunits from 1Sl Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality

Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution

Cloning and characterization of special HMW glutenin subunit genes from Aegilops longissima L. and their potential for wheat quality improvement

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