Molecular studies of meningococcal and pneumococcal meningitis patients in Ethiopia

Mihret, Wude; Brusletto, Berit; Øvstebø, Reidun; Trøseid, Anne-Marie Siebke; Norheim, Gunnstein; Merid, Yared; Kassu, Afework; Abebe, Workeabeba; Ayele, Samuel; Asres, Mezgebu Silamsaw; Yamuah, Lawrence; Aseffa, Abraham; Petros, Beyene; Caugant, Dominique A.; Brandtzæg, Petter

doi:10.1177/1753425918806363

Cited by 8 publications

(9 citation statements)

References 44 publications

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“…The overall incidence of meningitis has declined in recent decades due to the increasing use of meningococcal vaccines,[ 5 ] but the incidence of meningitis from bacterial strains not covered by vaccines has increased, and bacterial meningitis remains a significant public health problem. About 200,000 patients worldwide die from bacterial meningitis every year, and the mortality can be up to 60% in parts of sub-Saharan Africa and in poor and developing countrie s[ 6 , 7 ]. Patients who receive treatment have a mortality rate of about 10%, but survivors often experience serious sequelae, including limb amputation, neurological deficits, and other serious disabilitie s[ 8 ].…”

Section: Introductionmentioning

confidence: 99%

A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis

Fan

Tan

et al. 2020

BMC Infect Dis

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Background Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with a high mortality rate. Rapid and reliable diagnosis of bacterial meningitis is critical in clinical practice. However, this disease often occurs in economically depressed areas, so an inexpensive, easy to use, and accurate technology is needed. We performed a pooled-analysis to assess the potential of the recently developed loop-mediated isothermal amplification (LAMP) assay for detection of meningococcus. Methods Pubmed, Embase, and Web of Science were searched to identify original studies that used the LAMP assay to detect meningococcus. After pooling of data, the sensitivity and specificity were calculated, a summary receiver operating characteristic (SROC) curve was determined, and the area under the SROC curve was computed to determine diagnostic accuracy. Publication bias was assessed using Deek’s funnel plot. Results We examined 14 studies within 6 publications. The LAMP assay had high sensitivity (94%) and specificity (100%) in the detection of meningococcus in all studies. The area under the SROC curve (0.980) indicated high overall accuracy of the LAMP assay. There was no evidence of publication bias. Discussion The LAMP assay has accuracy comparable to bacterial culture and PCR for detection of meningococcus, but is less expensive and easier to use. We suggest the adoption of the LAMP assay to detect meningococcus, especially in economically depressed areas.

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Section: Introductionmentioning

confidence: 99%

A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis

Fan

Tan

et al. 2020

BMC Infect Dis

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“…Accordingly, 144 (43%) women and 188 (57%) men were included though the distribution varied between the different age groups (Table 1). The median ages of patients and healthy controls were 35 (IQR: 25-45) years and 25 (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32) years, respectively. The age and gender distribution of patients included in the present study is shown in Table 1.…”

Section: Sociodemographic and Clinical Characteristicsmentioning

confidence: 99%

CARD8 polymorphisms among bacterial meningitis patients in North-West Ethiopia

Belayneh,

Mengesha,

Idosa

et al. 2024

Preprint

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Background The severity of infectious disease outcomes is believed to depend on the virulence factors of the pathogen in combination with individuals’ unique immune response. CARD8 has been suggested to act as a regulator of the NLRP3 inflammasome, a major regulator of the innate immune proinflammatory response, and has been suggested to modulate the host response to common inflammatory diseases. In the present study, the C10X genetic polymorphism in the CARD8 gene was investigated in relation to bacterial meningitis. Methods A total of 400 clinically suspected meningitis patients hospitalized at the University of Gondar Comprehensive specialized Hospital were enrolled in the study. Cerebrospinal fluid (CSF) and blood samples were collected from all enrolled patients for laboratory investigations. The collected CSF was cultured, and all the results obtained from the culture were confirmed using direct RT‒PCR. Genotyping of whole-blood samples was performed for CARD8 gene using a TaqMan assay. The results were compared with apparently healthy controls and with PCR-negative meningitis suspected patients. Results Of the included patients, 57% were men and the most common clinical signs and symptoms were fever (81%), headache (80%), neck stiffness (76%), nausea (68%), and vomiting (67%). Microbiology culture identified seven patients with bacterial meningitis caused by Neisseria meningitidis (n = 4) and Streptococcus pneumoniae (n = 3). The RT-PCR revealed 39 positive samples for N. meningitidis (n = 10) and S. pneumoniae (n = 29). A total of 332 whole-blood samples were genotyped, with the following results: 151 (45.5%) C10X heterozygotes, 59 (17.7%) C10X homozygotes and 122 (36.7%) wild-type genotypes. The presence of the C10X polymorphism in the CARD8 gene was more prevalent in suspected meningitis patients than in healthy controls (OR 1.2; 1.00-1.5). Homozygote C10X polymorphic gene carriers were more susceptible to infectious disease. The presence of viable or active bacterial infection was found to be associated with the presence of heterozygous C10X carriers. Conclusions A greater proportion of C10X in the CARD8 gene in confirmed bacterial meningitis patients and clinically diagnosed meningitis patients than in healthy controls. Homozygote C10X polymorphic gene carriers were more susceptible to infectious disease than were heterozygote gene carriers and healthy controls.

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“…Recently, studies had reported that a variety of in ammatory factors were highly expressed in the cerebrospinal uid of patients with S. pneumoniae meningitis, including TNF-α, IL-6, IL-4 and IL-1β [4]. Yau found that TNF-α is an early in ammatory factor and can destroy the blood-brain barrier, then the plasma proteins could enter the brain tissue to aggravate brain damage [1].…”

Section: Introductionmentioning

confidence: 99%

The relationship between miR-340 and NOD2 may play an important role in Streptococcus pneumoniae-induced meningitis

Wang

Liu

et al. 2022

Preprint

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Bacterial meningitis is a serious infection of the central nervous system in children, with a high mortality rate of 12 % - 35 %. Two-thirds of bacterial meningitis cases in Europe and the United States are caused by Streptococcus pneumoniae. In this study, the overexpression of miR-340 and the knockdown of NOD2 could attenuate the microglial inflammation induced by Streptococcus pneumoniae and improve the nerve function in Streptococcus pneumoniae-induced meningitis mice. In contrast, inhibited miR-340 and overexpression of NOD2 had the opposite effects. Dual-luciferase reporter assay and recovery experiments showed that miR-340 could reduce the microglial inflammation induced by Streptococcus pneumoniae and improve the neurological damage caused by Streptococcus pneumoniae with regulating the expression of NOD2. MiR-340 and NOD2 may become the site of targeted therapy for Streptococcus pneumoniae meningitis.

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Molecular studies of meningococcal and pneumococcal meningitis patients in Ethiopia

Cited by 8 publications

References 44 publications

A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis

A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis

CARD8 polymorphisms among bacterial meningitis patients in North-West Ethiopia

The relationship between miR-340 and NOD2 may play an important role in Streptococcus pneumoniae-induced meningitis

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