Molecular Studies of the Flavinogenic Fungus Ashbya gossypii and the Flavinogenic Yeast Candida famata

Lyzak, Oleksii; Ledesma‐Amaro, Rodrigo; Dmytruk, Kostyantyn V.; Sibirny, Andriy А.; Revuelta, José Luis

doi:10.1007/978-3-319-58829-2_9

Cited by 3 publications

(3 citation statements)

References 61 publications

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“…Yeasts are metabolically versatile, low maintenance, easy to cultivate, safe, and have a high specific growth rate and short duplication time [ 11 , 12 ]. Some of the fluorochromes produced by yeasts are as follows: (1) ergosterol, a precursor of vitamin D 2 , is produced by Cystofilobasidium capitatum , Rhodotorula glutinis , and Sporobolomyces roseus ; (2) β-carotene, a precursor of vitamin A, is produced by the same yeast species mentioned previously ( C. capitatum , R. glutinis , and S. roseus ) [ 9 , 10 , 13 , 14 , 15 ], and (3) riboflavin, an essential vitamin for human nutrition, is produced by some yeast species such as Candida famata and Meyerozyma guilliermoindii (formerly known as Candida or Pichia guilliermondii ) [ 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

Jiménez-Nava,

Zepeda-Vallejo,

Santoyo-Tepole

et al. 2023

Biomolecules

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The yeast Hyphopichia wangnamkhiaoensis excretes a brilliant yellow fluorescent compound into its growth culture. In this study, we isolated and identified this compound using reverse-phase high-performance liquid chromatography-diode array detector (RP-HPLC-DAD) as well as 1H NMR and UV–Vis spectroscopy. Two of the three RP-HPLC-DAD methods used successfully separated the fluorescent compound and involved (1) a double separation step with isocratic flow elution, first on a C18 column and later on a cyano column, and (2) a separation with a linear gradient elution on a phenyl column. The wavelengths of maximum absorption of the fluorescent compound-containing HPLC fractions (~224, 268, 372, and 446 nm) are in good agreement with those exhibited by flavins. The 1H NMR spectra revealed methyl (δ 2.30 and 2.40) and aromatic proton (δ 7.79 and 7.77) signals of riboflavin. The 1H NMR spectra of the samples spiked with riboflavin confirmed that the brilliant yellow fluorescent compound is riboflavin. The maximum excitation and emission wavelengths of the fluorescent compound were 448 and 528 nm, respectively, which are identical to those of riboflavin.

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Section: Introductionmentioning

confidence: 99%

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

Jiménez-Nava,

Zepeda-Vallejo,

Santoyo-Tepole

et al. 2023

Biomolecules

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“…The advantage of using this marker is the change in colour of ade2 mutants, which greatly facilitates the selection of recombinant strains. However, currently, the efficiency of CRISPR-cas9 system in C. famata for auxotroph isolation still is quite low as compared with other yeast species (Lyzak, Ledesma-Amaro, Dmytruk, Sibirny, & Revuelta, 2017). The gene ble from Staphylococcus aureus conferring resistance to antibiotic phleomycin was the first dominant selectable marker used for transformation of this yeast species.…”

Section: Introductionmentioning

confidence: 99%

“…The gene ble from Staphylococcus aureus conferring resistance to antibiotic phleomycin was the first dominant selectable marker used for transformation of this yeast species. The gene SAT1 , encoding nourseothricin acetyl transferase, provided a selection of C. famata transformants with low concentration of antibiotic nourseothricin (2–4 mg/L; Lyzak et al, 2017). Moreover, Debaryomyces hansenii IMH3 gene conferring resistance to mycophenolic acid (15 mg/L) was reported as selectable marker for C. famata (Dmytruk, Yatsyshyn, Sybirna, Fedorovych, & Sibirny, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of new dominant selectable markers for the nonconventional yeasts Ogataea polymorpha and Candida famata

Bratiichuk

Kurylenko

Vasylyshyn

et al. 2020

Yeast

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Nonconventional yeast Candida famata and Ogataea polymorpha are interesting organisms for basic and applied studies. O. polymorpha is methylotrophic thermotolerant yeast capable of xylose alcoholic fermentation whereas C. famata is capable of riboflavin overproduction. Still, the new tools for molecular research of these species are needed. The aim of this study was to develop the new dominant selective markers for C. famata and O. polymorpha usable in metabolic engineering experiments. In this work, the BSD gene from Aspergillus terreus coding for blasticidin S deaminase, O. polymorpha AUR1 gene required for sphingolipid synthesis and IMH3 gene, which encodes IMP dehydrogenase, were tested as the new dominant selective marker genes. Our results showed that AUR1 and IMH3 genes could be used as dominant selective markers for O. polymorpha with frequencies of transformation of 40 and 20 transformants per microgram of DNA, respectively. The IMH3 gene was successfully used as the marker for construction of O. polymorpha strains with increased ethanol production from xylose due to overexpression of TAL1, TKL1 and AOX1 genes. The BSD gene from A. terreus, conferring resistance to blasticidin, was found to be efficient for selection of C. famata transformants.

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Revitalising Riboflavin: Unveiling Its Timeless Significance in Human Physiology and Health

Aragão,

Pires,

Santos-Buelga

et al. 2024

Foods

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Since the early twentieth century, research on vitamins has revealed their therapeutic potential beyond their role as essential micronutrients. Riboflavin, known as vitamin B2, stands out for its unique characteristics. Despite numerous studies, riboflavin remains vital, with implications for human health. Abundantly present in various foods, riboflavin acts as a coenzyme in numerous enzymatic reactions crucial for human metabolism. Its role in energy production, erythrocyte synthesis, and vitamin metabolism underscores its importance in maintaining homeostasis. The impact of riboflavin extends to neurological function, skin health, and cardiovascular well-being, with adequate levels linked to reduced risks of various ailments. However, inadequate intake or physiological stress can lead to deficiency, a condition that poses serious health risks, including severe complications. This underscores the importance of maintaining sufficient levels of riboflavin for general wellness. The essential role of riboflavin in immune function further emphasises its significance for human health and vitality. This paper examines the diverse effects of riboflavin on health and stresses the importance of maintaining sufficient levels for overall well-being.

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Molecular Studies of the Flavinogenic Fungus Ashbya gossypii and the Flavinogenic Yeast Candida famata

Cited by 3 publications

References 61 publications

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

RP-HPLC Separation and 1H NMR Identification of a Yellow Fluorescent Compound—Riboflavin (Vitamin B2)—Produced by the Yeast Hyphopichia wangnamkhiaoensis

Development of new dominant selectable markers for the nonconventional yeasts Ogataea polymorpha and Candida famata

Revitalising Riboflavin: Unveiling Its Timeless Significance in Human Physiology and Health

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