Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe

Criado–Fornelio, A.; Martı́nez-Marcos, Alino; Buling-Saraña, A.; Barba-Carretero, J.C.

doi:10.1016/s0304-4017(03)00078-5

Cited by 233 publications

(112 citation statements)

References 29 publications

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“…Nowadays, other small piroplasms are known to infect dogs including Theileria annae (ZAHLER et al, 2000), B. conradae (CONRAD et al, 1991;CONRAD, 2006), T. annulata (CRIADO et al, 2006), T. equi (CRIADO-FORNELIO et al, 2003) and Theileria sp. (MATJILA et al, 2008).…”

Section: Detection and Molecular Characterization Of Piroplasms Specimentioning

confidence: 99%

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Lemos

Cerqueira

Toma

et al. 2012

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.Keywords: Babesia vogeli, Rangelia vitalii, piroplasms, dogs, PCR. ResumoRangelia vitalii é um protozoário que infecta cães e foi descrito nas regiões Sul e Sudeste do Brasil. R. vitalii é filogeneticamente próxima à Babesia spp., mas dados deste misterioso parasito ainda são escassos. O objetivo deste trabalho foi detectar a presença de piroplasmas em cães naturalmente infectados no estado do Rio de Janeiro, através da amplificação do gene 18S rRNA pela PCR, clivagem com enzimas de restrição (RFLP) e caracterização genética através do sequenciamento. De 103 cães, sete (6,8%) foram positivos para Babesia spp. pela PCR. Os produtos amplificados foram digeridos por enzimas de restrição para a diferenciação das espécies de Babesia e uma amostra foi identificada como Babesia vogeli. O padrão de amplificação observado nas outras seis amostras não correspondeu ao padrão descrito para babesias que infectam cães. O sequenciamento das seis amostras confirmou ser uma espécie geneticamente idêntica a R. vitalii apresentando grande homologia (99-100%) com a sequência do sul do Brasil. Este estudo confirma a presença de Babesia vogeli e Rangelia vitalii infectando cães em Teresópolis, Rio de Janeiro, Brasil.

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Section: Detection and Molecular Characterization Of Piroplasms Specimentioning

confidence: 99%

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Lemos

Cerqueira

Toma

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…T. annulata was detected from an asymptomatic dog (Criado et al 2006), where as T. equi was detected from three asymptomatic dogs and one symptomatic dog (Criado-Fornelio et al 2003b). Beck et al (2009) on the basis of PCR and sequence analysis of a fragment of the ssrRNA gene found 3.42% prevalence of Babesia species in asymptomatic dogs.…”

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confidence: 99%

“…Other Theileria species that have been reported from dogs are Theileria annulata (Criado et al 2006) and Theileria equi (Criado-Fornelio et al 2003b). T. annulata was detected from an asymptomatic dog (Criado et al 2006), where as T. equi was detected from three asymptomatic dogs and one symptomatic dog (Criado-Fornelio et al 2003b).…”

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confidence: 99%

Evidence of new pathogenic Theileria species in dogs

Dixit

Dixit²,

Varshney³

2010

J Parasit Dis

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Babesiosis, caused by tick-borne haematozoan organisms of the genus Babesia, is a parasitic disease of domestic and wild mammals. Canine Babesidae have historically been classified as ''large Babesia'' (Babesia canis) and ''small Babesia'' (Babesia gibsoni) based on the size of their intraerythrocytic forms. Recent publications, however, suggest that the diversity of piroplasm species infecting dogs might be greater than previously appreciated. Sequencing and phylogenetic analysis of the ssrRNA gene has revealed that canine piroplasms are found in three clades, 'true' Babesia sp. (B. canis and B. gibsoni), Theileria annae and Theileria-like group (B. conradae). This newly recognised piroplasm T. annae appears to be hyperendemic in northwest Spain. The vector for this emergent canine infection remains undescribed, although the Ixodes hexagonus is suspected based on their presence upon the dogs in NW Spain and the relative absence of others. These observations have introduced a major change in the approach to the epidemiology of babesiosis in dogs. More clinical samples and data will need to be collected and analyzed to understand the importance of Theileria species in dogs.

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“…There may have been an error of detecting these parasites, especially in scanty infestations, considering the laboratory techniques that were used. Recently, more reliable molecular techniques for the diagnosis of parasitic infestations have been described (6,7,19,24,26).…”

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confidence: 99%