Molecular studies on genetic integrity of open-pollinating species rye (Secale cereale L.) after long-term genebank maintenance

Chebotar, S. V.; Röder, Marion S.; Korzun, Viktor; Saal, Bernhard; Weber, Wilhelm; Börner, Andreas

doi:10.1007/s00122-003-1366-1

Cited by 69 publications

(54 citation statements)

References 36 publications

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“…In addition to gene tagging they can be used to study genetic diversity, analysing high numbers of genotypes, as has been demonstrated for wheat by Huang et al (2002) and Röder et al (2002), who analysed about 1,000 genebank accessions and 500 European wheat varieties, respectively. The utilisation of rye microsatellites for studying the genetic integrity of genebank accessions of rye was demonstrated by Chebotar et al (2003).…”

Section: Discussionmentioning

confidence: 99%

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

et al. 2004

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The genetic map of rye contains predominantly restriction fragment length polymorphism (RFLP) markers but also a limited number of microsatellite markers, which are known to be more reliable and easier to apply. We report here the saturation of the genomic map of rye with additional microsatellite-derived markers that we obtained from the rye expressed sequence tag (EST) databases and the Gatersleben collection of wheat microsatellite markers (WMS). A total of 99 loci (39 EST and 60 WMS) were mapped into the RFLP frameworks of four rye mapping populations consisting of 139, 64, 58 and 60 RFLPs, respectively. For another ten WMS loci, which amplified PCR products not polymorphic in any of the mapping populations, chromosome and chromosome arm locations were determined using wheat-rye addition lines. Chromosomes 1R, 2R, 3R, 4R, 5R, 6R and 7R were enriched with 9, 19, 9, 13, 27, 16 and 16 microsatellite loci, respectively. The microsatellite loci mapped were evenly distributed along the chromosomes, which is important for the further application of these markers for gene mapping or diversity studies in rye. Forty-four of the WMS loci mapped in rye were found to be homologous to those mapped in bread wheat ( Triticum aestivum L.).

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Section: Discussionmentioning

confidence: 99%

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

et al. 2004

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“…In ex situ conservation procedures, accessions often need to be multiplied before being stored in germplasm banks and periodically regenerated in order to increase the amount of seed and rejuvenate it. During multiplication or regeneration, a certain level of genetic erosion can be observed and the genetic integrity and structure of an accession can be modified, due to occasional crossing with other accessions, genetic drift and/or selective effects related to the multiplication environment (Spagnoletti Zeuli et al 1995;Parzies et al 2000;Börner et al 2000;Chebotar et al 2003;Rice et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of in situ and ex situ conservation of crop diversity. What a Phaseolus vulgaris L. landrace case study can tell us

Negri

Tiranti

2010

Genetica

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The effectiveness of in situ (on-farm) and ex situ conservation strategies to maintain total genetic diversity was assessed in a threatened Phaseolus vulgaris L. landrace. Farmer seed lots (subpopulations) were sampled initially and then after in situ and ex situ multiplication (two locations). The number of plants used in the ex situ multiplications (120) was much larger than that normally used in germplasm bank procedures and the farmer seed lots were kept separate. In situ, the landrace was multiplied by each farmer with the usual population size. Eighty plants from the initial population, the in situ and the two ex situ multiplications were individually tested using 26 microsatellite markers. Most of the genetic parameters showed a consistent decline in the ex situ populations compared with the in situ population, with a notable loss of less frequent alleles. The differentiation among the farmer subpopulations increased when the multiplication took place outside of the adaptation area. Although 120 plants were multiplied in each ex situ cycle, a bottleneck effect was present. In addition, tests for neutrality detected three loci that are involved in pathogen response and are potentially under selective effects. The diversity conservation and the management practices of autogamous landrace crops are discussed.

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“…Studies made in other species showed that changes in allelic frequencies after regeneration were quite substantial in rye (Chebotar et al 2003), and barley (Parzies et al 2000), but smaller in maize and wheat (Reedy et al 1995;Börner et al 2000).…”

Section: Resultsmentioning

confidence: 96%

“…Microsatellite markers have been useful method to characterize, and study the diversity present in Brassica collections (Hasan et al 2005;Soengas et al 2006) and to study the genetic integrity of other outcrossing species (Chebotar et al 2003). The objective of this work is to study the effect of regeneration on the genetic integrity of B.…”

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confidence: 99%

Effect of regeneration procedures on the genetic integrity of Brassica oleracea accessions

et al. 2008

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A Brassica oleracea collection of landraces collected in the northwest of Spain is kept at the Gene Bank placed at 'Misión Biológica de Galicia'. Landraces of the collection are regenerated from time to time to restore the viability of the seed and to carry on field trials. The objective of this work is to study the effect of regeneration on the genetic integrity of three accessions of this collection, and to investigate the possible causes of the genetic changes observed. After characterizing the original populations and their following regenerated populations by 25 SSRs we concluded that there were significant changes in the population structure and the allelic frequency of individual loci due to the action of genetic drift, directional selection and probably assortative mating. Protocols to store and regenerate the accessions should be improved in order to avoid the effect of these forces in the genetic integrity of the collection.

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Molecular studies on genetic integrity of open-pollinating species rye (Secale cereale L.) after long-term genebank maintenance

Cited by 69 publications

References 36 publications

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

Effectiveness of in situ and ex situ conservation of crop diversity. What a Phaseolus vulgaris L. landrace case study can tell us

Effect of regeneration procedures on the genetic integrity of Brassica oleracea accessions

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