Molecular Studies on the Effect of some Auxins and Cytokinins on Vinca Rosae Grown by Plant Tissue Culture

Abstract

Vinca Rosea (Apocynacea) is an important medicinal plant. In this study, tissue culture propagation of vinca using explants from stems and leaves was investigated. Single and combined treatments of different phytohormones were added to Murashige and Skoog (MS) media. The genetic variation between cell lines derived from these explants was evaluated by using protein electrophoresis and determination of nucleic acids concentrations. The analysis of protein patterns showed that the number of protein bands in control was 16 bands, while in all treatments that number ranged between 4-33bands, regardless of explant origin. The polymorphism ratio was high in stem explants treatments through incubation period in comparison to the control, while that ratio decreased in leaves explants treatments. The treatment with Naphthalene acetic acid (NAA) hormone was the closest to the control sample. It was noticed that protein concentrations resulting from stems explants culture were higher than those recorded in leaves explants. Measurements of nucleic acids concentrations showed that the highest significant concentration for DNA and RNA resulted from stems explants treatments. In conclusion, it is recommended to use stem explants and NAA hormone to achieve a generation of plants similar to the origin when using tissue culture technique. ‫و‬ ‫الصانع‬ ‫إبراهيم‬ ‫سمية‬ ‫األحمدي‬ ‫منى‬ ‫و‬ ‫بحيري‬ ‫الدين‬ ‫محي‬ ( . 2020 .) ‫النسيجية‬ ‫الزراعة‬ ‫بواسطة‬ ‫النامية‬ ‫الونكة‬ ‫نبات‬ ‫على‬ ‫النباتية‬ ‫الهرمونات‬ ‫بعض‬ ‫لتأثير‬ ‫جزيئية‬ ‫دراسات‬ . ‫والطبيعية،‬ ‫األساسية‬ ‫العلوم‬ ‫فرع‬ ‫فيصل،‬ ‫امللك‬ ‫لجامعة‬ ‫العلمية‬ ‫املجلة‬ ‫الطباعة)‬ ‫(بانتظار‬ ‫العدد‬ ‫الطباعة)،‬ ‫(بانتظار‬ ‫املجلد‬ ‫و‬ ‫الصانع‬ ‫إبراهيم‬ ‫سمية‬ ‫األحمدي‬ ‫منى‬ ‫و‬ ‫بحيري‬ ‫الدين‬ ‫محي‬ ( . 2020 .) ‫النسيجية‬ ‫الزراعة‬ ‫بواسطة‬ ‫النامية‬ ‫الونكة‬ ‫نبات‬ ‫على‬ ‫النباتية‬ ‫الهرمونات‬ ‫بعض‬ ‫لتأثير‬ ‫جزيئية‬ ‫دراسات‬ . ‫والطبيعية،‬ ‫األساسية‬ ‫العلوم‬ ‫فرع‬ ‫فيصل،‬ ‫امللك‬ ‫لجامعة‬ ‫العلمية‬ ‫املجلة‬ ‫الطباعة)‬ ‫(بانتظار‬ ‫العدد‬ ‫الطباعة)،‬ ‫(بانتظار‬ ‫املجلد‬ ‫و‬ ‫الصانع‬ ‫إبراهيم‬ ‫سمية‬ ‫األحمدي‬ ‫منى‬ ‫و‬ ‫بحيري‬ ‫الدين‬ ‫محي‬ ( . 2020 .) ‫النسيجية‬ ‫الزراعة‬ ‫بواسطة‬ ‫النامية‬ ‫الونكة‬ ‫نبات‬ ‫على‬ ‫النباتية‬ ‫الهرمونات‬ ‫بعض‬ ‫لتأثير‬ ‫جزيئية‬ ‫دراسات‬ . ‫والطبيعية،‬ ‫األساسية‬ ‫العلوم‬ ‫فرع‬ ‫فيصل،‬ ‫امللك‬ ‫لجامعة‬ ‫العلمية‬ ‫املجلة‬ ‫الطباعة)‬ ‫(بانتظار‬ ‫العدد‬ ‫الطباعة)،‬ ‫(بانتظار‬ ‫املجلد‬ ‫و‬ ‫الصانع‬ ‫إبراهيم‬ ‫سمية‬ ‫األحمدي‬ ‫منى‬ ‫و‬ ‫بحيري‬ ‫الدين‬ ‫محي‬ ( . 2020 .) ‫النسيجية‬ ‫الزراعة‬ ‫بواسطة‬ ‫النامية‬ ‫الونكة‬ ‫نبات‬ ‫على‬ ‫النباتية‬ ‫الهرمونات‬ ‫بعض‬ ‫لتأثير‬ ‫جزيئية‬ ‫دراسات‬ . ‫والطبيعية،‬ ‫األساسية‬ ‫العلوم‬ ‫فرع‬ ‫فيصل،‬ ‫امللك‬ ‫لجامعة‬ ‫العلمية‬ ‫املجلة‬ ‫الطباعة)‬ ‫(بانتظار‬ ‫العدد‬ ‫الطباعة)،‬ ‫(بانتظار‬ ‫املجلد‬

Effect of Growth Regulators and Nacl Stress on Callus Formation and Alkaloids Production on Vinca Rosea Plant (Catharanthus Roseus L)

“…Different research and studies for new vinca alkaloid applications will be carried out in this regard. So, we conclude from this research that the different concentrations of growth hormones affect the differentiation and formation of callus where it was found that the use of hormonal BA and 2, 4-D is what gives the highest efficiency and vitality of the resulting callus [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22].…”