Molecular study of carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenems and determining their clonal relationship using pulsed-field gel electrophoresis

Shahcheraghi, Fereshteh; Aslani, Mohammad Mehdi; Mahmoudi, Hassan; Karimitabar, Zahra; Solgi, Hamid; Bahador, Abbas; Alikhani, Mohammad Yousef

doi:10.1099/jmm.0.000467

Cited by 19 publications

(14 citation statements)

References 17 publications

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“…Epidemiological investigation such as PFGE is used for the bacteria isolates outbreak and also isolates transmission among patients, and within hospital wards. 23 In this study, PFGE analysis showed that K. pneumoniae was divided into 11 clusters and 3 singletons. The largest clusters had 4 similar isolates, and the other clusters had 2 or 3 identical isolates (Figure 1).…”

Section: Discussionmentioning

confidence: 67%

“…The rate of resistance to imipenem and meropenem in our strains is in line with other studies in Tehran, Egypt, and China. [23][24][25] Carbapenemase such as bla NDM is a plasmid-borne gene; 25 so, it may have readily been transmitted to other susceptible bacteria and make them resistant to carbapenemase, which might lead to failure in controlling spread of these bacteria in the hospital environment. This study investigated the prevalence of carbapenemase genes (bla NDM and bla OXA-48 were 45% and 10%, respectively); these genes were isolated from different wards, especially the ICU.…”

Section: Discussionmentioning

confidence: 99%

“…Transmission of microbial infections and resistant genes to carbapenems was reported in hospital wards. 23,26,27 The role of infection control and careful surveillance is very important in hospitals in order to reduce infections and antibiotic resistance. We can use this strategy to prevent and manage antimicrobial resistance in nosocomial settings and reduce the outbreaks.…”

Section: Discussionmentioning

confidence: 99%

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Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran

Hashemizadeh¹,

Hosseinzadeh²,

Azimzadeh³

et al. 2020

IDR

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Background: Klebsiella pneumoniae is an important cause of healthcare-associated infection. Carbapenemases have increasingly been reported in Enterobacteriaceae, especially in K. pneumoniae. Propose: The objective of this study was to determine antibiotic resistance patterns, and the molecular epidemiology of multidrug resistant K. pneumoniae isolates, obtained from hospitalized patients in Shiraz, Iran. Methods: In this study, 60 K. pneumoniaeisolates were collected from Nemazee and Faghihi referral hospitals. Antibiotic susceptibility testing and MIC were performed by disk diffusion test and Epsilometer (E)-test strips, respectively. Carbapenemase genes were identified by polymerase chain reaction and sequencing. Then, clonal relationships were analyzed, using PFGE. Results: Thirty-three out of 60 K. pneumoniae isolates were resistant to carbapenems. Among the isolates, 86.6% were multidrug resistant (MDR). Polymyxin B (18.3%) and tigecycline (23.3%) were shown to be the most active agents against K. pneumoniae isolates. In our study, the high prevalence of bla NDM (45%) and bla OXA-48 (10%) was detected. Conclusion: The results of this study revealed the widespread carbapenemase gene between different wards in hospitals as a risk factor for treatment options. PFGE analysis showed 11 clusters and 3 singletons based on an 80% similarity level. Also, PFGE analysis showed that there were similar genetic patterns among K. pneumoniae isolates and these patterns were responsible for the distribution of infection in hospitals.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran

Hashemizadeh¹,

Hosseinzadeh²,

Azimzadeh³

et al. 2020

IDR

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“…The supernatant was used as a template for polymerase chain reaction (PCR) assay. PCR assays were carried out for detection of KPC, NDM, and OXA-48 genes using a set of specific primers as previously described (Table 1) (2,(18)(19)(20)(21). Standard strains for blaOXA-48, blaNDM, and blaKPC genes were provided by the Pasteur Institute of Iran.…”

Section: Molecular Detection Of Genes Encoding Carbapenemasesmentioning

confidence: 99%

“…Nosocomial infections, a major public healthcare problem, are more prevalent in developed countries because of the related mortality and socioeconomic costs (2). Enterobacteriaceae is one of the major causative agents of nosocomial infections (3,4).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City

Jalalvand¹,

Shayanfar²,

Shahcheraghi³

et al. 2020

Iran J Pathol

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Tracking Multidrug-Resistant Klebsiella pneumoniae from an Italian Hospital: Molecular Epidemiology and Surveillance by PFGE, RAPD and PCR-Based Resistance Genes Prevalence

et al. 2018

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Antimicrobial-resistant Klebsiella pneumoniae represent a global public health concern. K. pneumoniae strains isolated during 2010 and 2014-2016 within a single hospital of Molise Region, Central Italy, were analyzed testing antimicrobial susceptibility, clonality by pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD)-PCR, and prevalence of carbapenem resistance genes by PCR. Forty isolates (23 wild-type in 2010 and 17 non-wild-type in 2014-2016) were collected from hospitalized patients (65.2 ± 18.1 years old, 75% male, 80% from intensive care unit-ICU). K. pneumoniae showed multidrug-resistant profiles and 15 resistotypes were identified (discriminatory power D = 0.88). The 69.6 and 17.4% of isolates in 2010 resulted intermediate and resistant to imipenem, respectively, and 91.3% was sensitive to meropenem, while 88.2% of isolates of 2014-2016 were resistant to both antibiotics. PFGE identified 16 clusters versus 23 by RAPD, 26 pulsotypes versus 33 RAPD patterns (D ≥ 0.97). PFGE separated strains according to isolation period and identified an outbreak occurred in the ICU during December 2014 and January 2015. No strains harbored bla, bla, bla, and bla genes, as well as AmpC plasmid-mediated beta-lactamases genes. Only K. pneumoniae isolated during 2014-2016 were bla positive. Prevalence of bla was 87 and 76.5% during 2010 and 2014-2016, respectively. No strains colistin-resistant harbored mcr-1 plasmid-mediated resistance gene. The study findings underline an increased circulation of multidrug-resistant K. pneumoniae within the hospital, and the acquisition of carbapenem resistance mechanism. The implementation of surveillance and molecular characterization of isolates are needed to identify outbreaks, reduce the spread of resistance, and guide empirical therapy.

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Molecular study of carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenems and determining their clonal relationship using pulsed-field gel electrophoresis

Cited by 19 publications

References 17 publications

<p>Dissemination Pattern of Multidrug Resistant Carbapenemase Producing <em>Klebsiella pneumoniae</em> Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran</p>

<p>Dissemination Pattern of Multidrug Resistant Carbapenemase Producing <em>Klebsiella pneumoniae</em> Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran</p>

Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City

Tracking Multidrug-Resistant Klebsiella pneumoniae from an Italian Hospital: Molecular Epidemiology and Surveillance by PFGE, RAPD and PCR-Based Resistance Genes Prevalence

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