Molecular study of colistin resistant clinical isolates of Enterobacteriaceae species

Zaki, Maysaa El Sayed; ElKheir, Nermen Abou; Mofreh, Mohamed

doi:10.15761/jcmm.1000103

Cited by 13 publications

(18 citation statements)

References 27 publications

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“…This result indicates that many significant Gram-negative isolates pose mechanism for colistin resistance. Various other authors have also reported colistin resistance among a variety of Gram-negative isolates including Acinetobacter and Pseudomonas [17], E. coli, K. oxytoca, K. pneumoniae, Acinetobacter iwoffii, E. agglomerans, Enterobacter cloacae, P. putida, and Pseudomonas aeruginosa [18], Klebsiella, E. coli, Enterobacter, and Acinetobacter [19], and Klebsiella [20]. Many colistin-resistant clinical isolates were resistant to a wide variety of antimicrobial agents, including penicillins, cephalosporins, monobactams, carbapenems, aminoglycosides, quinolones, nitrofurans, etc.…”

Section: Discussionmentioning

confidence: 88%

Colistin resistance among Gram-negative isolates in Nepal: A review

Devkota

Paudel²

2020

J. Biomed. Sci.

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Background: Colistin resistance among Gram-negative isolates is a tremendous public health problem, and there are very few studies in Nepal about these pathogens. Hence, this review provides comprehensive data on colistin resistance among Gram-negative isolates from various samples in Nepal. Methods: Articles reporting colistin resistance among various Gram-negative isolates from Nepal before July 2019 were selected; analyzed and relevant data was collected. Results: Colistin resistance was low among clinical isolates (less than 6%) in comparison to food and animal isolates (up to 69%). A wide variety of clinical isolates were colistin-resistant in comparison to food and animal isolates. Many of these isolates were highly drug-resistant and also harbored various drug-resistant determinants. Conclusion: Increased colistin resistance among Gram-negative pathogens is a serious concern. Screening of these isolates in clinical settings, animal farms, and food industries, as well as cautious use of colistin in both clinical and animal farms, is imminent.

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Section: Discussionmentioning

confidence: 88%

Colistin resistance among Gram-negative isolates in Nepal: A review

Devkota

Paudel²

2020

J. Biomed. Sci.

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“…Studies from North Africa also reported humans as reservoir for COL-resistant organisms [ 28 , 60 , 76 , 77 , 89 – 97 ]. In Egypt, 20 strains (six E .…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

“…coli , nine Pseudomonas aeruginosa , three K . pneumoniae , and two Acinetobacter baumannii ) carrying mcr -1 and one tigecyline-resistant Enterobacter hormaechei (isolated during 2015) carrying mcr -9 on IncHI2 plasmid were detected among 285 clinical MDR isolates (7%) from individuals [ 89 – 95 ], suggesting low circulation of mcr genes among Enterobacteriaceae and non- Enterobacteriaceae causing difficult-to-treat diseases in Egypt, and that organisms producing MCR-9 had been present in North Africa, since at least half a decade. It also suggested that organisms in the ESKAPE ( Enterococcus , Staphylococcus , K .…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

“…baumannii ) in Africa portends a substantial challenge to public health (due to increased disease burden and cost of hospital stay/treatment) because various intrinsic and acquired traits enable these organisms to exhibit resistance to many antibiotics, including the critically important ones, and they represent the most causes of difficult-to-treat nosocomial infections worldwide [ 98 , 99 ]. Some of the mcr -1-positive strains were recovered from individuals without a travel history [ 91 ], persons with healthcare-associated infections [ 89 ], and those who took antibiotics one month before hospitalization [ 95 ]. Thus, suggesting that antibiotic consumption is a putative risk for the development of carbapenem-resistant and COL-resistant bacteria, and that these organisms are disseminated from the community to the hospitals and vice versa in Africa.…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

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Prevalence and Traits of Mobile Colistin Resistance Gene Harbouring Isolates from Different Ecosystems in Africa

Anyanwu

Okpala

Chah

et al. 2021

BioMed Research International

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The mobile colistin resistance (mcr) gene threatens the efficacy of colistin (COL), a last-line antibiotic used in treating deadly infections. For more than six decades, COL is used in livestock around the globe, including Africa. The use of critically important antimicrobial agents, like COL, is largely unregulated in Africa, and many other factors militate against effective antimicrobial stewardship in the continent. Currently, ten mcr genes (mcr-1 to mcr-10) have been described. In Africa, mcr-1, mcr-2, mcr-3, mcr-5, mcr-8, and mcr-9 have been detected in isolates from humans, animals, foods of animal origin, and the environment. These genes are harboured by Escherichia coli, Klebsiella, Salmonella, Citrobacter, Enterobacter, Pseudomonas, Aeromonas, Alcaligenes, and Acinetobacter baumannii isolates. Different conjugative and nonconjugative plasmids form the backbone for mcr in these isolates; however, mcr-1 and mcr-3 have also been integrated into the chromosome of some African strains. Insertion sequences (ISs) (especially ISApl1), either located upstream or downstream of mcr, class 1 integrons, and transposons, are drivers of mcr in Africa. Genes coding multi/extensive drug resistance and virulence are colocated with mcr on plasmids in African strains. Transmission of mcr to/among African strains is nonclonal. Contact with mcr-habouring reservoirs, the consumption of contaminated foods of animal/plant origin or fluid, animal-/plant-based food trade and travel serve as exportation, importation, and transmission routes of mcr gene-containing bacteria in Africa. Herein, the current status of plasmid-mediated COL resistance in humans, food-producing animals, foods of animal origin, and environment in Africa is discussed.

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“…Colistin-resistant Pseudomonas aeruginosa (CRPA) strains have been detected in many infections, including wound and bloodstream. (Lee and Ko 2014;El Sayed Zaki et al 2018;Osei Sekyere 2019). Resistance to polymyxins most frequently results from the modification of the LPS caused by the addition of a 4-amino-4-deoxy-l-arabinose moiety in the lipid A structure and the underlying mutations are frequently tracked to the PmrAB or PhoPQ twocomponent regulators, which in turn lead to the activation of the pmrACADTEF operon (Osei Sekyere et al 2016;Chalmers et al 2018).…”

Section: Introductionmentioning

confidence: 99%

New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay

Tahmasebi

Dehbashi

Arabestani

2020

Lett Appl Microbiol

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Colistin‐resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high‐resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known genomic DNA (gDNA) and plasmid DNA (pDNA) concentrations, extracted from P. aeruginosa NCTC13715 and P. aeruginosa NCTC10728 were prepared and tested by melting curve analysis and HRM assay. Data analysis was performed using the Step‐One Plus Software v2.3 and hrm Software v3.0.1. The results of the melt curve analysis and HRM showed a very similar melt peak for all positive controls with a melt temperature that ranged from 88·1°C to 88·6°C for the 16srRNA, 90·0°C to 90·05°C for the mcr‐1 and 91·2°C to 91·7°C for the pmrA gene. Furthermore, the data indicated that the HRM approach has the sensitivity to detect 100 CFU per ml for the 16srRNA gene, 101 CFU per ml for the pmrA gene, and 103 CFU per ml for the mcr‐1 gene. According to our findings, it was concluded that the HRM method could detect 100 to 103 CFU per ml of P. aeruginosa gDNA and pDNA. Therefore, CRPA strains can be identified with high sensitivity and specificity by HRM assay. Significance and Impact of the Study The highlight of our research is about the detection of bacteria resistance genes to antibiotics by advanced molecular methods, which means high‐resolution melting curve (HRM) analysis. We determined that the HRM method in identifying colistin‐resistant P. aeruginosa has high accuracy and speed, and with high sensitivity and specificity.

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Molecular study of colistin resistant clinical isolates of Enterobacteriaceae species

Abstract: Background: There is recent concern about the development of colistin resistance that may disturb the antibiotics therapy used for extended beta lactamase producing Enterobacteriaceae species.

Cited by 13 publications

References 27 publications

Colistin resistance among Gram-negative isolates in Nepal: A review

Colistin resistance among Gram-negative isolates in Nepal: A review

Prevalence and Traits of Mobile Colistin Resistance Gene Harbouring Isolates from Different Ecosystems in Africa

New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay

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