Molecular Study of Cryptosporidium spp. in Dogs from Southwest of Iran

Tavalla, Mehdi; Kord, Elham; Abdizadeh, Rahman; Asgarian, Fatemeh

doi:10.5812/jjm.43412

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Furthermore, the prevalence of Cryptosporidium spp. obtained in China (3.8%), Iran (8%), and Zambia (5.9%) is as low as those in developed countries [7,19,30]. The difference in the prevalence may be attributed to geographic location and standard of living, which determines the health care given to the pets.…”

Section: Discussionmentioning

confidence: 96%

Prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs in Enugu State, Nigeria

et al. 2019

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Aims: Cryptosporidiosis is an important zoonotic disease of major public and veterinary concern. The disease affects humans and a variety of animal species including the domestic dog. This study aimed to determine the prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs from different homes and those presented at veterinary hospitals and clinics in Enugu State, Nigeria. Materials and Methods: A total of 203 fresh fecal samples were collected from domestic dogs in six local government areas in Enugu State from February 2015 to August 2015. All the samples were examined using the formol-ether sedimentation method. Fecal smears were then stained by the modified Ziehl-Neelsen technique and examined under direct light microscopy. Results: A total of 74 (36.5%) dogs were infected with Cryptosporidium spp. oocysts. There was a strong association (p<0.05) between the presence of Cryptosporidium spp. oocysts and management practices. However, there was no statistically significant association (p>0.05) between the presence of Cryptosporidium spp. oocysts and age, sex, and fecal consistency. Conclusion: The findings of this work suggest that domestic dogs in Enugu State harbor and shed Cryptosporidium spp. oocysts in the environment, especially those managed semi-intensively. Such fecal shedding is particularly so and of greater zoonotic and epidemiological importance in animals that do not show clinical signs and therefore not treated. They, therefore, pose a greater public health risk, especially to immune-compromised humans and animals. Public education on the zoonotic implication of this protozoan infection is of paramount importance in Enugu State, in particular, and Nigeria, in general, considering the closeness of dogs and man.

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Section: Discussionmentioning

confidence: 96%

Prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs in Enugu State, Nigeria

et al. 2019

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“… Microscopic Arzamani [ 62 ] 2016 Khorasan Dog 32 1 3.1 Cryptosporidium spp. Microscopic Tavalla [ 63 ] 2017 Khuzestan Dog 350 43 12.3 Cryptosporidium spp. PCR Mohaghegh [ 64 ] 2017 Kermanshah Dog 301 72 23.9 Cryptosporidium spp.…”

Section: Resultsmentioning

confidence: 99%

Cryptosporidium animal species in Iran: a systematic review and meta-analysis

et al. 2020

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Background Cryptosporidiosis is an acute and short-term infection which can lead to severe diarrhea (intestinal cryptosporidiosis) associated with a persistent cough in the host with immune system defect. This systematic review and meta-analysis was conducted to estimate the prevalence of animal Cryptosporidium species and the corresponding epidemiological aspects in Iran. Methods In this study, all original research articles relating to the animal cryptosporidiosis in Iran were collected from reliable databases using keywords. A meta-analysis was conducted separately for each subgroup, and heterogeneity among the studies was performed using the Q and I2 tests. Furthermore, it should be noticed that the significance level in the statistical analysis with the Comprehensive Meta-analysis software was considered to be less than 0.05. Finally, meta-analysis results were shown in forest plot with a 95% CI. Results In total, 4795 studies were included in the initial screening. Duplicated or non-original studies and the ones which did not meet our considered criteria were excluded from the list. Out of the 100 articles included in our first list for the meta-analysis, 40, 16, 13, 10, 9, 7, and 5 were done on cattle and calves, birds, dogs, sheep, rodents, camels, and horses, respectively. The prevalence rate of cryptosporidiosis among the birds, horses, rodents, camels, dogs, cattle, and sheep in Iran was estimated to be 7.5%, 19.5%, 20.8%, 8.4%, 4.9%, 14.4%, and 9.1%, respectively. Conclusion The different Cryptosporidium species have been found in different regions of Iran. Geographical region, climate, and domestic animals are considered as factors responsible for animal cryptosporidiosis prevalence in the area. Moreover, this parasite is zoonotic which causes disease in animals as well as humans which can result in economic loss.

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Molecular Identification ofCryptosporidiumspp. in Iranian Dogs Using Seminested PCR: A First Report

Ranjbar

Mirhendi

Izadi

et al. 2018

Vector-Borne and Zoonotic Diseases

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Cryptosporidium is a parasitic protozoon with a wide range of vertebrate hosts. Cryptosporidiosis has been reported from numerous countries, including Iran. Molecular identification can be applied to characterize Cryptosporidium, of which there are over 30 species and 50 genotypes. Herein, we report the genetic diversity of Cryptosporidium spp. in Iranian dogs for the first time based on 18S ribosomal RNA gene sequencing. One hundred forty fecal samples of herd dogs were collected from Isfahan, central Iran. The samples were concentrated using sucrose flotation and subjected to Kinyoun staining. DNA extraction of positive samples was performed, and molecular diagnosis was carried out using highly specific seminested PCR for the characterization of Cryptosporidium species. Finally, sequencing and DNA analysis were performed to identify Cryptosporidium species. A total of 2.14% of herd dogs were positive for cryptosporidiosis in both microscopy and molecular methods. In all cases, the causative agent was identified as Cryptosporidium parvum. Dogs associated with positive samples had been in close relationship with livestock. Cryptosporidiosis in the herd dogs in Isfahan could be due to their close contact with animals, particularly cattle and sheep. Given that dogs with cryptosporidiosis lack clinical symptoms, they are a potential source of zoonotic transmission of this disease as they are companion animals for humans. Dogs with cryptosporidiosis are a potential source of the zoonotic transmission of this disease.

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Molecular Study of Cryptosporidium spp. in Dogs from Southwest of Iran

Cited by 5 publications

References 37 publications

Prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs in Enugu State, Nigeria

Prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs in Enugu State, Nigeria

Cryptosporidium animal species in Iran: a systematic review and meta-analysis

Molecular Identification ofCryptosporidiumspp. in Iranian Dogs Using Seminested PCR: A First Report

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