Molecular study of human astrovirus in Egyptian children with acute gastroenteritis

Zaki, Maysaa El Sayed; Mashaly, Ghada El-Saeed; Alsayed, Mona Abdel Latif; Nomir, Manal

doi:10.18683/germs.2020.1202

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…Among the 100 studied children with AGE, norovirus and astrovirus were detected in 27% and 12%, respectively. These prevalence rates were similar to previous studies from Egypt [ 32 ], the Republic of Congo (10.3%) [ 33 ], and India (12.5%) [ 34 ]. Nevertheless, the rates were higher than reported in a previous study from Kenya (6.3%) [ 35 ].…”

Section: Discussionsupporting

confidence: 91%

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Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus

et al. 2022

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Background and aim Human parechovirus (HPeV) has emerged as a pathogen associated with acute gastroenteritis (AGE). Aim To detect the presence of HPeV in the stool samples from Egyptian children with AGE seeking care and the possibility of its co-infection with other enteric viruses. Methodology One hundred stool samples were collected from children attending Mansoura University Children's Hospital with AGE. HPeV and astrovirus were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). At the same time, detection of rotavirus antigen and norovirus was achieved by enzyme-linked immunosorbent assay and rapid immunochromatographic method, respectively. Results The most frequently detected virus was rotavirus (39%), followed by norovirus (27%), HPeV (19%), and astrovirus (12%). Interestingly, the single infection with HPeV was 5%. Among the 19 HPeV positive samples, the co-infection of HPeV with other enteric viruses was detected in 9(43.9%) for rotavirus, 7(36.8%) for norovirus, 2(10.5%) for astrovirus, in 3(15.8%) for rotavirus and norovirus and 1(5.3%) for norovirus and astrovirus. Regarding the clinical presentation, there was no significant difference between children infected with HPeV alone and those infected with viruses other than HPeV alone; fever (p = 0.3), vomiting (p = 0.12), abdominal pain (p = 0.12), and grades of severity (P = 0.82). HPeV alone infected children were of mild severity (60%), and their main presenting symptom was fever (60%). Conclusions Detection of HPeV as a single viral pathogen in the stool of some children with AGE showed that this virus could be a causative agent of AGE in Egyptian children. Therefore, HPeV could be included as one of the viruses screened for AGE diagnosis in children in Egypt.

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Section: Discussionsupporting

confidence: 91%

“…The prevalence of these viruses was lower than others obtained in Egypt (28%) and Nigeria (40.4%) [ 36 , 37 ]. The variation in the prevalence rates can be attributed to the difference between geographical regions and the difference in socioeconomic factors [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus

et al. 2022

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“…The astrovirus rate in this study was 11.7%. The presence of astrovirus was investigated by El Sayed Zaki et al 23 by RT-PCR in 100 children with AGE in Egypt, and it was found at a rate of 11.1% consistent with this study. In the study of Lekana-Douki et al investigating the molecular epidemiology of gastroenteritis viruses in children under 5 years old in Gabon, astrovirus was detected to be 6.3%.…”

Section: Discussionsupporting

confidence: 87%

Investigation of Children with Acute Gastroenteritis by Multiplex PCR Method in Central Part of Turkey

Yılmaz

Kaya

Özdemir

2022

J Pediatr Infect Dis

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Objective Gastroenteritis is a disease that affects all age groups, especially children, and causes high mortality and morbidity in all countries. The most common agents of acute gastroenteritis are viral agents. As a result, millions of diarrhea attacks and hospital admissions occur worldwide every year due to viral gastroenteritis. This study uses the multiplex polymerase chain reaction (PCR) method to investigate the viruses that are the causative agents of viral gastroenteritis in the pediatric patient group in Konya, Turkey. Methods Stool samples of 94 patients aged 0 to 18 years sent from Emergency clinics and Pediatric outpatient clinics, Meram Medical Faculty Hospital Pediatric clinics, Konya Necmettin Erbakan University to Medical Microbiology Laboratory with a diagnosis of gastroenteritis between February and December 2018 were included in the study. Stool samples were stored at –80°C until the time of the analysis. Deoxyribonucleic acid/ribonucleic acid isolation from stool samples was performed with EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany) using an automatic extraction system (BioRobot EZ1 system, Qiagen). The presence of astrovirus, rotavirus, adenovirus, norovirus (GI, GII), and sapovirus agents was investigated by the multiplex PCR method (Fast Track Diagnostics, Luxembourg) viral gastroenteritis kit. Results Viral gastroenteritis agents were detected in 56.3% of the patients. One viral agent was detected in 47 (50%) of these patients and at least two viral agents in 6 (6.3%) of them. Norovirus GII was detected in 20 (21.2%) of the children included in the study, adenovirus in 13 (13.8%), rotavirus in 11 (12.8%), astrovirus in 11 (11.7%), sapovirus in 4 (4.2%), and norovirus GI in 1 (1.06%). When the distribution of viral agents was examined by months, the most number of agents were observed (21; 35%) in May, followed by April and June (12; 20%). Considering the distribution of the prevalence of the agents by age, it was seen to be mainly between 0 and 12 months (42%). Conclusion Considering that the most common viral agent in our region is norovirus GII, it will be useful to investigate the norovirus that is not routinely examined in children who are admitted to clinics with the complaint of gastroenteritis. It will be appropriate to examine routinely adenovirus, rotavirus, and norovirus in the laboratory, especially in children with diarrhea and vomiting in the winter and spring months.

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“…The dry conditions in rural areas can lead to increased water storage which then increases the risk of water contamination, as well as less water accessible for personal hygiene, which increases the risk of potential diseases [ 2 , 26 ]. Virus detection may have been influenced by seasonality; Sapovirus and Rotavirus are more prevalent in cold months [ 72 , 73 ], while Astrovirus infections are more likely to occur in spring and summer [ 74 ]. In the current study, Norovirus was more prevalent in specimens collected from patients presenting with diarrhoea during the rainy season, and this is consistent with previous studies [ 75 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

High Burden of Co-Infection with Multiple Enteric Pathogens in Children Suffering with Diarrhoea from Rural and Peri-Urban Communities in South Africa

et al. 2023

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Infectious diarrhoea contributes to high morbidity and mortality in young children from sub-Saharan Africa. The aim of this study was to assess the prevalence of single and multiple diarrhoeal-causing pathogen combinations in children suffering from diarrhoea from rural and peri-urban communities in South Africa. A total of 275 diarrhoea stool specimens were collected between 2014 and 2016 from Hospitals and Primary Health Care clinics. The BioFire® FilmArray® Gastrointestinal panel was used to simultaneously detect 22 diarrhoea pathogens (viruses, bacteria, parasites) known to cause diarrhoea. A total of 82% (226/275) enteric pathogens were detected in the stool specimens. The two most detected bacterial, viral and parasitic pathogens each included: EAEC (42%), EPEC (32%), Adenovirus F40/41 (19%), Norovirus (15%), Giardia (8%) and Cryptosporidium (6%), respectively. Single enteric pathogen infections were recorded in 24% (65/275) specimens with EAEC, and Norovirus was found in 26% (17/65) and 14% (9/65) of the specimens, respectively. Multiple enteric pathogen combinations were recorded in 59% (161/275) of the stool specimens with 53% (85/161) containing two pathogens, 22% (35/161) containing three pathogens and 25% (41/161) containing four or more pathogens. The results from this study demonstrated the complex nature of pathogen co-infections in diarrhoeal episodes which could have an impact on treatment effectiveness.

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Molecular study of human astrovirus in Egyptian children with acute gastroenteritis

Cited by 8 publications

References 27 publications

Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus

Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus

Investigation of Children with Acute Gastroenteritis by Multiplex PCR Method in Central Part of Turkey

High Burden of Co-Infection with Multiple Enteric Pathogens in Children Suffering with Diarrhoea from Rural and Peri-Urban Communities in South Africa

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